mouse anti-Monkeypox virus A35R monoclonal antibody (101) 5800

$450.00

Antibody summary

Mouse monoclonal to Monkeypox A35R
Suitable for: WB,ELISA
Reacts with: A35R
Isotype: IgG2a
1 mg

SKU: 5800parent Category: antibody Tag: monkeypox virus

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2a
clonality	monoclonal
concentration	lyophilized
applications	ELISA, WB
reactivity	A35R, monkeypox
available sizes	1 mg

mouse anti-Monkeypox virus A35R monoclonal antibody (101) 5800

antibody
Database link: monkeypox A35R Q8V4U4/NP_536572.1
Tested applications WB, ELISA
Recommended dilutions to be determined by user
Immunogen Peripheral blood mononuclear cells sequenced
Size and concentration 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, pH 7.2
Purity affinity purified
Clonality monoclonal
Isotype IgG2a
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2a control

target relevance
Reactive to the EV membrane protein by ORF A35R of Monkey Pox Virus.This antibody is expressed in HEK293 cells and and was purified using a combination of Protein A/G chromatography. It is reactive against VACV antigen and lysate, CPXV lysate, MPXV lysate, and VARV antigen and lysate. This product is sold for research use only. Optimal working concentrations should be determined by each researcher.
Structure The monkeypox virus is a large, double-stranded DNA virus within the Orthopoxvirus genus, known for its complex structure and multiple subunits that contribute to its infectivity and immune evasion. The virus is brick-shaped, with dimensions of approximately 200-250 nanometers, and is enveloped by a lipid bilayer that contains various glycoproteins essential for host cell entry. Within this outer envelope lies the core, or nucleoid, which houses the viral genome. This core is flanked by two lateral bodies that carry enzymes and proteins vital for early viral replication and manipulation of the host's cellular environment. The viral genome itself is extensive, encoding over 190 proteins that are crucial for the virus's life cycle, from entry and replication to immune evasion and virulence. Key structural proteins encoded by the monkeypox virus genome include B6R, A35R, and M1R, among others. The B6R protein is an envelope glycoprotein involved in the formation of the extracellular enveloped virus (EEV), a form that enhances viral spread within the host by evading the immune system. The A35R protein plays a role in modulating the host immune response, particularly by interfering with the host's ability to mount an effective defense, thereby allowing the virus to establish a more persistent infection. The M1R protein, also known as the core protein, is crucial for maintaining the structural integrity of the viral core, ensuring that the viral DNA is protected and efficiently replicated within the host cell. Together, these and other viral proteins orchestrate a complex interplay of mechanisms that enable the monkeypox virus to infect host cells, replicate, and spread, making it a highly efficient pathogen.
Biotechnology Diagnosis of monkeypox involves a combination of clinical assessment, laboratory testing, and advanced molecular techniques. Clinically, healthcare providers assess symptoms such as fever, swollen lymph nodes, and a distinctive rash that progresses through stages like macules, papules, vesicles, and pustules. Due to the similarity of these symptoms to other conditions like chickenpox, laboratory confirmation is crucial. Polymerase chain reaction (PCR) testing is the most reliable method, detecting viral DNA in samples from skin lesions, blood, or other bodily fluids. Additionally, serological methods, including antibody enzyme-linked immunosorbent assay (ELISA), are used to detect specific antibodies against the monkeypox virus. These tests help determine if an individual has been previously exposed to the virus or is in the later stages of infection. ELISA and other serological assays are valuable for both acute and retrospective diagnosis, providing insights into the immune response and potential immunity. In some cases, electron microscopy or virus isolation in cell culture may be utilized, particularly in research settings, to confirm the presence of the virus and study its characteristics.
Involvement in disease Monkeypox is a viral zoonotic disease that presents with a range of symptoms, often beginning with flu-like signs such as fever, chills, headache, and muscle aches. This initial phase is followed by the development of swollen lymph nodes, which is a distinguishing feature of monkeypox compared to similar diseases. The hallmark of the disease is a characteristic rash that progresses through distinct stages: macules (flat spots), papules (raised bumps), vesicles (fluid-filled blisters), and pustules (pus-filled lesions). These skin lesions typically appear first on the face and then spread to other parts of the body, including the arms and legs. The disease can vary in severity, with some cases being mild and self-limiting, while others may be more severe and lead to complications such as secondary bacterial infections or, in rare instances, more serious outcomes like pneumonia or encephalitis. The duration of the illness is usually around 2 to 4 weeks, and while most individuals recover fully, severe cases, particularly in individuals with weakened immune systems, can be life-threatening.

Data

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Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.