Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human glioma stained with MGMT antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells

mouse anti-MGMT monoclonal antibody (ZM314) 6255

Price range: $160.00 through $528.00

Antibody summary

Mouse monoclonal to MGMT
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Colon carcinoma
Visualization: Nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

available sizes

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SKU: 6255parent Categories: antibody, Zeta IHC antibodies Tags: anatomy, MGMT, Neuropathology/Endocrine

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-MGMT monoclonal antibody ZM314 6255

antibody
Database link: human P16455
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Colon carcinoma
Immunogen Purified recombinant human MGMT protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Methylated-DNA--protein-cysteine methyltransferase (EC 2.1.1.63) (6-O-methylguanine-DNA methyltransferase) (MGMT) (O-6-methylguanine-DNA-alkyltransferase)
Gene names MGMT,MGMT
Protein family MGMT family
Mass 21646Da
Function FUNCTION: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
Catalytic activity CATALYTIC ACTIVITY: Reaction=a 6-O-methyl-2'-deoxyguanosine in DNA + L-cysteinyl-[protein] = S-methyl-L-cysteinyl-[protein] + a 2'-deoxyguanosine in DNA; Xref=Rhea:RHEA:24000, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132, Rhea:RHEA-COMP:11367, Rhea:RHEA-COMP:11368, ChEBI:CHEBI:29950, ChEBI:CHEBI:82612, ChEBI:CHEBI:85445, ChEBI:CHEBI:85448; EC=2.1.1.63; Evidence={ECO:0000255\|PROSITE-ProRule:PRU10017}; CATALYTIC ACTIVITY: Reaction=a 4-O-methyl-thymidine in DNA + L-cysteinyl-[protein] = a thymidine in DNA + S-methyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:53428, Rhea:RHEA-COMP:10131, Rhea:RHEA-COMP:10132, Rhea:RHEA-COMP:13555, Rhea:RHEA-COMP:13556, ChEBI:CHEBI:29950, ChEBI:CHEBI:82612, ChEBI:CHEBI:137386, ChEBI:CHEBI:137387; EC=2.1.1.63; Evidence={ECO:0000255\|PROSITE-ProRule:PRU10017};
Subellular location SUBCELLULAR LOCATION: Nucleus.
Target Relevance information above includes information from UniProt accession: P16455
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human glioma stained with MGMT antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear staining of tumor cells

FAQ & Publications

Frequently Asked Questions

What applications is the mouse anti-MGMT monoclonal antibody (ZM314) suitable for?

This antibody is suitable for Immunohistochemistry (IHC) applications, specifically for formalin-fixed, paraffin-embedded human tissues.

How should the mouse anti-MGMT monoclonal antibody be stored to maintain stability?

For short-term storage, keep the antibody at 2-8°C. For longer-term storage, it should be stored at -20°C while avoiding repeated freeze/thaw cycles.

What species does the mouse anti-MGMT monoclonal antibody (ZM314) react with?

This antibody specifically reacts with human MGMT protein.

What is the recommended dilution range for using the concentrated mouse anti-MGMT monoclonal antibody in IHC?

The recommended dilution for the concentrated antibody in Immunohistochemistry is between 1:100 and 1:200.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.