| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Measles Nucleoprotein |
| available sizes | 100 µg |
mouse anti-Measles Nucleoprotein monoclonal antibody (2F3) 5089
$520.00
Antibody summary
- Mouse monoclonal to Measles Nucleoprotein
- Suitable for: WB,ICC,ELISA
- Isotype: IgG1
- 100 µg
mouse anti-Measles Nucleoprotein monoclonal antibody (2F3) 5089
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions ELISA: use at 0.1-1.0ug/ml Immunoblotting: User should use at 1-5ug/ml. A band of ~60kDa is detected Immunocytochemistry: use at 2- 10ug/ml on virus-infected cells. These are recommended concentrations. End user should determine optimal concentrations for the |
| Immunogen Recombinant Measles virus nucleoprotein. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names Nucleoprotein (Nucleocapsid protein) (NP) (Protein N) |
| Gene names N,N NP |
| Protein family Paramyxoviruses nucleocapsid family |
| Mass 58131Da |
| Function Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter (By similarity). The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P (By similarity). Participates, together with P, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place (By similarity). N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response (PubMed:15914856). Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest (By similarity). |
| Subellular location Virion. Host cytoplasm . |
| Structure Homomultimer; forms the nucleocapsid (By similarity). Binds to viral genomic RNA (By similarity). N0 interacts (via Ncore) with the phosphoprotein (via N-terminus); this interaction allows P to chaperon N0 to avoid N polymerization before encapsidation (PubMed:26719278). Interacts as N-RNA template with the phosphoprotein (via C-terminus); this interaction positions the polymerase on the template (By similarity). Interacts with the phosphoprotein; this interaction leads to the formation of membraneless organelles that function as viral replication factories (By similarity). Interacts with human FCGR2B protein (PubMed:15914856). Interacts with human PPIA/CYPA and PPIB/CYPB (By similarity). |
| Target Relevance information above includes information from UniProt accession: Q89933 |
| The UniProt Consortium |
Data
| No results found |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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