| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Measles Nucleoprotein |
| available sizes | 100 µg |
mouse anti-Measles Nucleoprotein monoclonal antibody (2F3) 5089
$520.00
Antibody summary
- Mouse monoclonal to Measles Nucleoprotein
- Suitable for: WB,ICC,ELISA
- Isotype: IgG1
- 100 µg
mouse anti-Measles Nucleoprotein monoclonal antibody (2F3) 5089
| antibody |
|---|
| Tested applications WB,ICC/IF,ELISA |
| Recommended dilutions ELISA: use at 0.1-1.0ug/ml Immunoblotting: User should use at 1-5ug/ml. A band of ~60kDa is detected Immunocytochemistry: use at 2- 10ug/ml on virus-infected cells. These are recommended concentrations. End user should determine optimal concentrations for the |
| Immunogen Recombinant Measles virus nucleoprotein. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names Nucleoprotein (Nucleocapsid protein) (NP) (Protein N) |
| Gene names N,N NP |
| Protein family Paramyxoviruses nucleocapsid family |
| Mass 58131Da |
| Function Forms the helical nucleocapsid (NC) in a ratio of 1 N per 6 ribonucleotides, protecting the genome from nucleases. The nucleocapsid (NC) has a helical structure with either 12.35 or 11.64 N per turn, approximately 20 nm in diameter, with a hollow central cavity approximately 5 nm in diameter (By similarity). The encapsidated genomic RNA serves as template for transcription and replication; encapsidation by N is coupled to RNA synthesis. Forms the encapsidation complex with the phosphoprotein protein P. Before encapsidation, the newly synthesized free N protein, so-called N0, is chaperoned by P (By similarity). Participates, together with P, in the formation of viral factories (viroplasms), which are large inclusions in the host cytoplasm where replication takes place (By similarity). N is released in the blood following lysis of measles infected cells, it interacts then with human FCGR2B on immune cells, inducing apoptosis and blocking inflammatory immune response (PubMed:15914856). Ntail binds to a protein on human thymic epithelial cells, termed Nucleoprotein Receptor (NR), inducing growth arrest (By similarity). |
| Subellular location Virion. Host cytoplasm . |
| Structure Homomultimer; forms the nucleocapsid (By similarity). Binds to viral genomic RNA (By similarity). N0 interacts (via Ncore) with the phosphoprotein (via N-terminus); this interaction allows P to chaperon N0 to avoid N polymerization before encapsidation (PubMed:26719278). Interacts as N-RNA template with the phosphoprotein (via C-terminus); this interaction positions the polymerase on the template (By similarity). Interacts with the phosphoprotein; this interaction leads to the formation of membraneless organelles that function as viral replication factories (By similarity). Interacts with human FCGR2B protein (PubMed:15914856). Interacts with human PPIA/CYPA and PPIB/CYPB (By similarity). |
| Target Relevance information above includes information from UniProt accession: Q89933 |
| The UniProt Consortium |
Data
| No results found |
Publications
Publications
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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