| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2a |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | HTLV-1 Tax |
| available sizes | 100 µg |
mouse anti-HTLV-1 Tax monoclonal antibody (1A3) 8868
$520.00
Antibody summary
- Mouse monoclonal to HTLV-1 Tax
- Suitable for: WB,IHC,ELISA
- Isotype: IgG2a
- 100 µg
mouse anti-HTLV-1 Tax monoclonal antibody (1A3) 8868
| antibody |
|---|
| Tested applications WB,IHC,IHC,ELISA |
| Recommended dilutions ELISA: use at 0.1-1.0ug/ml Immunoblotting: User should use at 2-10ug/ml. A band of ~42kDa is detected. Immunohistochemistry: use at 2- 20ug/ml. These are recommended concentrations. End user should determine optimal concentrations for their applications. |
| Immunogen Recombinant full-length HTLV- Tax protein. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG2a |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG2a - Isotype Control |
| target relevance |
|---|
| Protein names Protein Tax-1 (Protein X-LOR) (Protein PX) (Trans-activating transcriptional regulatory protein of HTLV-1) |
| Gene names Tax,Tax |
| Protein family Deltaretrovirus Tax protein family |
| Mass 39471Da |
| Function Transcriptional activator that governs the viral transcription from the 5'LTR via the recruitment of dimers of host phosphorylated CREB1. Together they bind cAMP response elements within the viral promoter and mediate high-level viral transcription (PubMed:8970957, PubMed:22789739). Increases host CREB1 O-GlcNAcylation to further increase 5'LTR transactivation (PubMed:28742148). Modulates also the expression of cellular genes leading to the deregulation of T-cell proliferation, perturbing the integrity of cell cycle checkpoints, the DNA damage response and apopototic pathways. Acts as an ubiquitin E3 ligase and stimulates host IKK complex by catalyzing the assembly of free mixed-linkage polyubiquitin chains, resulting in constitutive activation of the transcription factor NF-kappa-B (PubMed:28103322, PubMed:27082114). Inhibits the host nonsense-mediated mRNA decay (NMD), a cellular process that can actively degrade mRNAs by interacting with host UPF1 (PubMed:27082114). |
| Subellular location Host nucleus. Host cytoplasm. Note=Shuttles from the host nucleus to the cytoplasm. Found predominantly in the nucleus, where it is equally distributed between the nucleoplasm and the nuclear matrix. |
| Structure Homodimer. Interacts with host CREB1 (PubMed:8970957). Interacts with host DLG1, IKBKG, KDM4A, MAGII3 and TAX1BP1. Recruits the coactivators CREBBP, EP300 and PCAF (PubMed:10766811). Interaction with human CARM1 enhances Tax transactivation and promotes methylation of histone H3 (PubMed:17005681). Interacts with host SUV39H1 protein, leading to abrogate Tax transactivation of HTLV-1 LTR (PubMed:16409643). Interaction with human CREB coactivators, CRTC1/TORC1, CRTC2/TORC2 and CRTC3/TORC3 enhances Tax transactivation (PubMed:15466468, PubMed:16809310). Interacts with host UPF1; this interaction inhibits the host nonsense-mediated mRNA decay (NMD). Interacts (via N-terminus) with host PLSCR1; this interaction negatively regulates Tax transactivation activity by altering its subcellular distribution and homodimerization (PubMed:22789739). |
| Post-translational modification Phosphorylation at Thr-48 results in the loss of NF-kappa-B activation function. Phosphorylation at Thr-215 results in loss of CREB and NF-B responsive promoters activation. Phosphorylation at Thr-184 and Ser-336 have no effect on these Tax functions. Phosphorylation of either Ser-300 or Ser-301 is necessary for localization to nuclear bodies. Thr-48, Thr-184, Thr-215 and Ser-336 are highly phosphorylated, whereas Ser-300 or Ser-301 are only rarely phosphorylated.; Modified by host ubiquitin related modifier 1/URM1, resulting in a redistribution of Tax to the cytoplasm. |
| Target Relevance information above includes information from UniProt accession: P03409 |
| The UniProt Consortium |
Data
| No results found |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
|---|
| Western blot IHC |
Documents
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| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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