Skip to content

mouse anti-Heme Oxygenase-1 monoclonal antibody (GTS-1) 2852

$509.00

Antibody summary

  • Mouse monoclonal to Heme Oxygenase-1
  • Suitable for: ELISA
  • Isotype: IgG1
  • 100 µg
SKU: 2852parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Heme Oxygenase-1

available sizes

100 µg

Available product – mouse anti-Heme Oxygenase-1 monoclonal antibody (GTS-1) 2852

antibody
Tested applications
ELISA
Recommended dilutions
Immunoblotting: use at 5-10ug/mL. A band of ~33kDa is detected.

Immunohistochemistry: use at 5-10ug/mL on human frozen or paraffin-embedded tissue. Antigen retrieval with 0.4ug/mL Proteinase K.

These are recommended concentrations.

Endusers should determine optimal concentra
Immunogen
Microsomal fraction of transformed mouse T-cell transfected with rat heme oxygenase- 1 (WR19LrHO-1).
Size and concentration
100µg and
Form
lyophilized
Storage Instructions
Stock solution of 2mg/ml should be stored for one (1) year at -20°C in appropriate aliquots to avoid multiple freeze-thaw cycles or at 4°C for 6 months with the addition of 0.1% sodium a
Storage buffer
PBS, pH 7.4, 1% BSA., lyophilized.
Purity
immunogen affinty purifcation
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
Heme oxygenase 1 (HO-1) (EC 1.14.14.18) [Cleaved into: Heme oxygenase 1 soluble form]
Gene names
HMOX1,HMOX1 HO HO1
Protein family
Heme oxygenase family
Mass
32819Da
Function
FUNCTION: [Heme oxygenase 1]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron (PubMed:11121422, PubMed:19556236, PubMed:7703255). Affords protection against programmed cell death and this cytoprotective effect relies on its ability to catabolize free heme and prevent it from sensitizing cells to undergo apoptosis (PubMed:20055707). {ECO:0000269|PubMed:11121422, ECO:0000269|PubMed:19556236, ECO:0000269|PubMed:7703255, ECO:0000303|PubMed:20055707}.; FUNCTION: [Heme oxygenase 1]: (Microbial infection) During SARS-COV-2 infection, promotes SARS-CoV-2 ORF3A-mediated autophagy but is unlikely to be required for ORF3A-mediated induction of reticulophagy. {ECO:0000269|PubMed:35239449}.; FUNCTION: [Heme oxygenase 1 soluble form]: Catalyzes the oxidative cleavage of heme at the alpha-methene bridge carbon, released as carbon monoxide (CO), to generate biliverdin IXalpha, while releasing the central heme iron chelate as ferrous iron. {ECO:0000269|PubMed:7703255}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=heme b + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = biliverdin IXalpha + CO + Fe(2+) + H(+) + 3 H2O + 3 oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:21764, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17245, ChEBI:CHEBI:29033, ChEBI:CHEBI:57618, ChEBI:CHEBI:57991, ChEBI:CHEBI:58210, ChEBI:CHEBI:60344; EC=1.14.14.18; Evidence={ECO:0000269|PubMed:11121422, ECO:0000269|PubMed:19556236, ECO:0000269|PubMed:7703255}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:21765; Evidence={ECO:0000305|PubMed:7703255};
Subellular location
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:19556236, ECO:0000269|PubMed:22419571, ECO:0000269|PubMed:27184847}; Single-pass type IV membrane protein {ECO:0000255}; Cytoplasmic side {ECO:0000269|PubMed:22419571}.
Tissues
TISSUE SPECIFICITY: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). {ECO:0000269|PubMed:20855888}.
Structure
SUBUNIT: (Microbial infection) Interacts with SARS-CoV-2 ORF3A protein; the interaction promotes ORF3A-induced autophagy but is unlikely to be involved in ORF3A-mediated induction of reticulophagy. {ECO:0000269|PubMed:35239449}.; SUBUNIT: Homodimer and higher order homooligomer (PubMed:19556236). Oligomerization is crucial for its stability and function in the endoplasmic reticulum (PubMed:19556236). Interacts with FLVCR2; this interaction is potentiated in the presence of heme. {ECO:0000250|UniProtKB:P14901, ECO:0000269|PubMed:19556236}.
Post-translational modification
PTM: A soluble form arises by proteolytic removal of the membrane anchor. {ECO:0000305|PubMed:7703255}.
Domain
DOMAIN: Th
Target Relevance information above includes information from UniProt accession : P09601
The UniProt Consortium

Data

benchmark-antibodies_anti-heme_oxygenase-1_antibody_2852_1.jpg
Immunoblotting: use at 5-10ug/ml. A band of ~33kDa is detected. Detection of rat HO-1 with #2852 at 5ug/ml.
benchmark-antibodies_anti-heme_oxygenase-1_antibody_2852_2.jpg
Immunohistochemistry: use at 5-10ug/ml on human frozen or paraffin-embedded tissue. Antigen retrieval with 0.4ug/ml Proteinase K.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
ELISA

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.