| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Glutathione-S-Transferase (GST) |
| available sizes | 100 µg |
mouse anti-GST monoclonal antibody (3G10) 4677
$503.00
Antibody summary
- Mouse monoclonal to GST
- Suitable for: WB,IP
- Isotype: IgG1
- 100 µg
mouse anti-GST monoclonal antibody (3G10) 4677
| antibody |
|---|
| Tested applications WB,IP |
| Recommended dilutions Immunoblotting: use at 0.2-2ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their applications. |
| Immunogen GST protein of Schistosoma japonicum expressed in E. coli. |
| Size and concentration 100µg and 1 mg/mL |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20°C. |
| Storage buffer PBS, pH 7.4. |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names Glutathione S-transferase class-mu 28 kDa isozyme (GST 28) (EC 2.5.1.18) (Sj28 antigen) (Sj28GST) |
| Protein family GST superfamily, Mu family |
| Mass 23393Da |
| Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.; GST isoenzymes appear to play a central role in the parasite detoxification system. Other functions are also suspected including a role in increasing the solubility of haematin in the parasite gut. |
| Catalytic activity CATALYTIC ACTIVITY: Reaction=glutathione + RX = a halide anion + an S-substituted glutathione + H(+); Xref=Rhea:RHEA:16437, ChEBI:CHEBI:15378, ChEBI:CHEBI:16042, ChEBI:CHEBI:17792, ChEBI:CHEBI:57925, ChEBI:CHEBI:90779; EC=2.5.1.18; |
| Structure Homodimer. |
| Target Relevance information above includes information from UniProt accession: P26624 |
| The UniProt Consortium |
Data
 |
| GST tag antibody [3G10] detects GST tag protein by Western blot analysis. A. Non-induced cell lysate from E. coli expressing GST-tagged proteins B. IPTG-induced cell lysate from E. coli expressing GST-tagged proteins 10 % SDS-PAGE GST tag antibody [3G10] (4677) dilution: 1:10000 |
 |
| GST tag antibody [3G10] detects GST tag protein by Western blot analysis. A. 2 µg His-recombinant protein B. 2 µg GST-recombinant protein C. 1 µg GST-recombinant protein D. 0.5 µg GST-recombinant protein 10 % SDS-PAGE GST tag antibody [3G10] (4677) dilution: 1:5000 |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
|---|
Protocols
| relevant to this product |
|---|
| Western blot |
Documents
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| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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