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mouse anti-Fen-1 monoclonal antibody (4E7) 3657

$503.00

Antibody summary

  • Mouse monoclonal to Fen-1
  • Suitable for: WB,ICC/IF,IP
  • Isotype: IgG1
  • 100 µg
SKU: 3657parent Category: Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Fen-1

available sizes

100 µg

mouse anti-Fen-1 monoclonal antibody (4E7) 3657

antibody
Tested applications
WB,ICC/IF,IP
Recommended dilutions
Immunoblotting: use at 1-5 ug/mL.

Positive controls: MCF-7 cells and recombinant fusion protein.
Immunogen
Recombinant human FEN-1 protein (aa 1-380) purified from baculovirus-infected Sf-9 cells.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.
Storage buffer
10 mM PBS, pH 7.4.
Purity
immunogen affinty purifcation
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
Flap endonuclease 1 (FEN-1) (EC 3.1.-.-) (DNase IV) (Flap structure-specific endonuclease 1) (Maturation factor 1) (MF1) (hFEN-1)
Gene names
FEN1,FEN1 RAD2
Protein family
XPG/RAD2 endonuclease family, FEN1 subfamily
Mass
42593Da
Function
Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. It enters the flap from the 5'-end and then tracks to cleave the flap base, leaving a nick for ligation. Also involved in the long patch base excision repair (LP-BER) pathway, by cleaving within the apurinic/apyrimidinic (AP) site-terminated flap. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
Subellular location
[Isoform 1]: Nucleus, nucleolus. Nucleus, nucleoplasm. Note=Resides mostly in the nucleoli and relocalizes to the nucleoplasm upon DNA damage.; [Isoform FENMIT]: Mitochondrion .
Structure
Interacts with PCNA (PubMed:9305916, PubMed:11430825, PubMed:26760506, PubMed:15616578). Three molecules of FEN1 bind to one PCNA trimer with each molecule binding to one PCNA monomer (PubMed:15616578). PCNA stimulates the nuclease activity without altering cleavage specificity (PubMed:15616578). The C-terminal domain binds EP300; can bind simultaneously to both PCNA and EP300 (PubMed:11430825). Interacts with DDX11; this interaction is direct and increases flap endonuclease activity of FEN1 (PubMed:18499658). Interacts with WDR4; regulating its endonuclease activity (PubMed:26751069). Interacts with POLB (PubMed:19336415, PubMed:26760506).
Post-translational modification
Acetylated by EP300. Acetylation inhibits both endonuclease and exonuclease activity. Acetylation also reduces DNA-binding activity but does not affect interaction with PCNA or EP300.; Phosphorylation upon DNA damage induces relocalization to the nuclear plasma. Phosphorylation at Ser-187 by CDK2 occurs during late S-phase and results in dissociation from PCNA.; Methylation at Arg-192 by PRMT5 impedes Ser-187 phosphorylation and increases interaction with PCNA.
Target Relevance information above includes information from UniProt accession: P39748
The UniProt Consortium

benchmark-antibodies_anti-fen-1_antibody_3657_1.jpg
Whole cell extract (30 µg) was separated by 12% SDS-PAGE, and the membrane was blotted with FEN1 antibody (3657) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
benchmark-antibodies_anti-fen-1_antibody_3657_2.jpg
Immunofluorescence analysis of paraformaldehyde-fixed MCF7, using FEN1 [4E7](3657) antibody at 1:200 dilution.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

relevant to this product
Western blot
IHC
ICC
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