mouse anti-Estrogen Receptor beta monoclonal antibody (6A12) 8974

$503.00

Antibody summary

Mouse monoclonal to Estrogen Receptor beta
Suitable for: WB,ICC/IF,ChIP,IHC
Isotype: IgG2b
100 µg

SKU: 8974parent Category: antibody Tags: Estrogen Receptor beta, monoclonal, mouse

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	Estrogen Receptor β
available sizes	100 µg

mouse anti-Estrogen Receptor beta monoclonal antibody (6A12) 8974

antibody

Tested applications WB,IHC,IHC,ICC/IF,IP,ChIP
Recommended dilutions Immunoblotting: use at 1-5 ug/mL. Positive controls: Native receptor from human thymus, spleen, ovary, and testis or recombinant protein.
Immunogen 6-His fusion protein containing the region encoding aa 1-153 of human estrogen receptor-b (ER-b) expressed in E. coli.
Size and concentration 100µg and lot specific
Form liquid
Storage Instructions This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Storage buffer PBS, pH 7.4
Purity protein affinty purification
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG2b - Isotype Control

target relevance
Protein names Estrogen receptor beta (ER-beta) (Nuclear receptor subfamily 3 group A member 2)
Gene names ESR2,ESR2 ESTRB NR3A2
Protein family Nuclear hormone receptor family, NR3 subfamily
Mass 59216Da
Function FUNCTION: Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560). {ECO:0000269\|PubMed:20074560, ECO:0000269\|PubMed:29261182, ECO:0000269\|PubMed:30113650, ECO:0000269\|PubMed:9325313}.; FUNCTION: [Isoform 2]: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. {ECO:0000269\|PubMed:9671811}.
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00407, ECO:0000269\|PubMed:19126643, ECO:0000269\|PubMed:20074560}.
Tissues TISSUE SPECIFICITY: [Isoform 1]: Expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors. {ECO:0000269\|PubMed:9636657}.; TISSUE SPECIFICITY: [Isoform 2]: Expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus. {ECO:0000269\|PubMed:9636657}.; TISSUE SPECIFICITY: [Isoform 4]: Expressed in the testis. {ECO:0000269\|PubMed:9636657}.; TISSUE SPECIFICITY: [Isoform 5]: Expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus. {ECO:0000269\|PubMed:9636657}.; TISSUE SPECIFICITY: [Isoform 6]: Expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. {ECO:0000269\|PubMed:9636657}.
Structure SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA1, NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with UBE1C. Interacts with AKAP13. Interacts with DNTTIP2. Interacts with isoform 4 of TXNRD1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes, including cyclin-D1/CCND1 AP-1 promoter. Interacts with DNAAF4 (By similarity). Interacts with PRMT2. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with RBM39, in the presence of estradiol (E2) (By similarity). {ECO:0000250\|UniProtKB:O08537, ECO:0000250\|UniProtKB:Q62986, ECO:0000269\|PubMed:10681503, ECO:0000269\|PubMed:11113208, ECO:0000269\|PubMed:11579095, ECO:0000269\|PubMed:12039952, ECO:0000269\|PubMed:15047147, ECO:0000269\|PubMed:15199063, ECO:0000269\|PubMed:19126643, ECO:0000269\|PubMed:20074560, ECO:0000269\|PubMed:9267036}.; SUBUNIT: [Isoform 2]: Preferentially forms a heterodimer with ESR1 rather than ESR2 isoform 1 and inhibits DNA-binding by ESR1. {ECO:0000269\|PubMed:9671811}.
Post-translational modification PTM: Phosphorylation at Ser-87 and Ser-105 recruits NCOA1. {ECO:0000269\|PubMed:15862947}.
Target Relevance information above includes information from UniProt accession : Q92731
The UniProt Consortium

Data

benchmark-antibodies_anti-estrogen_receptor_beta_antibody_8974_1.jpg

Non-transfected (-) and transfected (-) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12] (8974) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmid	title	authors	citation

Protocols

relevant to this product
Western blot IHC ICC

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