| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2b |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Estrogen Receptor β |
| available sizes | 100 µg |
mouse anti-Estrogen Receptor beta monoclonal antibody (6A12) 8974
$503.00
Antibody summary
- Mouse monoclonal to Estrogen Receptor beta
- Suitable for: WB,ICC/IF,ChIP,IHC
- Isotype: IgG2b
- 100 µg
mouse anti-Estrogen Receptor beta monoclonal antibody (6A12) 8974
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF,IP,ChIP |
| Recommended dilutions Immunoblotting: use at 1-5 ug/mL. Positive controls: Native receptor from human thymus, spleen, ovary, and testis or recombinant protein. |
| Immunogen 6-His fusion protein containing the region encoding aa 1-153 of human estrogen receptor-b (ER-b) expressed in E. coli. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. |
| Storage buffer PBS, pH 7.4 |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG2b |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG2b - Isotype Control |
| target relevance |
|---|
| Protein names Estrogen receptor beta (ER-beta) (Nuclear receptor subfamily 3 group A member 2) |
| Gene names ESR2,ESR2 ESTRB NR3A2 |
| Protein family Nuclear hormone receptor family, NR3 subfamily |
| Mass 59216Da |
| Function Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner (PubMed:20074560).; [Isoform 2]: Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. |
| Subellular location Nucleus . |
| Tissues [Isoform 1]: Expressed in testis and ovary, and at a lower level in heart, brain, placenta, liver, skeletal muscle, spleen, thymus, prostate, colon, bone marrow, mammary gland and uterus. Also found in uterine bone, breast, and ovarian tumor cell lines, but not in colon and liver tumors.; [Isoform 2]: Expressed in spleen, thymus, testis and ovary and at a lower level in skeletal muscle, prostate, colon, small intestine, leukocytes, bone marrow, mammary gland and uterus.; [Isoform 4]: Expressed in the testis.; [Isoform 5]: Expressed in testis, and at a lower level in spleen, thymus, ovary, mammary gland and uterus.; [Isoform 6]: Expressed in testis, placenta, skeletal muscle, spleen and leukocytes, and at a lower level in heart, lung, liver, kidney, pancreas, thymus, prostate, colon, small intestine, bone marrow, mammary gland and uterus. Not expressed in brain. |
| Structure Binds DNA as a homodimer. Can form a heterodimer with ESR1. Interacts with NCOA1, NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with UBE1C. Interacts with AKAP13. Interacts with DNTTIP2. Interacts with isoform 4 of TXNRD1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes, including cyclin-D1/CCND1 AP-1 promoter. Interacts with DNAAF4 (By similarity). Interacts with PRMT2. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with RBM39, in the presence of estradiol (E2) (By similarity).; [Isoform 2]: Preferentially forms a heterodimer with ESR1 rather than ESR2 isoform 1 and inhibits DNA-binding by ESR1. |
| Post-translational modification Phosphorylation at Ser-87 and Ser-105 recruits NCOA1. |
| Involvement in disease DISEASE: Ovarian dysgenesis 8 (ODG8) [MIM:618187]: An autosomal dominant form of ovarian dysgenesis, a disorder characterized by lack of spontaneous pubertal development, primary amenorrhea, uterine hypoplasia, and hypergonadotropic hypogonadism as a result of streak gonads. Note=The disease may be caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: Q92731 |
| The UniProt Consortium |
 |
| Non-transfected (-) and transfected (-) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor beta antibody [6A12] (8974) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. |
Publications
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| We haven't added any publications to our database yet. | |||
| relevant to this product |
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| Western blot IHC ICC |
| # | SDS | Certificate | |
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