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mouse anti-Estrogen Receptor α monoclonal antibody (1F3) 3616

$503.00

Antibody summary

  • Mouse monoclonal to Estrogen Receptor α
  • Suitable for: WB,IHC-Fr,FACS,IP
  • Isotype: IgG1
  • 100 µg
SKU: 3616parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Estrogen Receptorα

available sizes

100 µg

Available product – mouse anti-Estrogen Receptor α monoclonal antibody (1F3) 3616

antibody
Tested applications
WB,IHC,IHC,IP
Recommended dilutions
Immunoblotting: use at 1-5ug/mL.

Immunohistochemistry: frozen sections, use at 1-10ug/mL.FACS: use at 1-10ug/mL.

Immunoprecipitation: use at 1-5ug/mL.

Positive controls: MCF-7 cells and recombinant protein.

These are recommended concentrations.

Endusers shoul
Immunogen
6-His fusion protein containing the region encoding aa 1-190 of human estrogen receptor-a (ER-a) expressed in E. coli.
Size and concentration
100µg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.
Storage buffer
PBS, pH 7.4, 1mg/ml.
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
Estrogen receptor (ER) (ER-alpha) (Estradiol receptor) (Nuclear receptor subfamily 3 group A member 1)
Gene names
ESR1,ESR1 ESR NR3A1
Protein family
Nuclear hormone receptor family, NR3 subfamily
Mass
66216Da
Function
FUNCTION: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Ligand-dependent nuclear transactivation involves either direct homodimer binding to a palindromic estrogen response element (ERE) sequence or association with other DNA-binding transcription factors, such as AP-1/c-Jun, c-Fos, ATF-2, Sp1 and Sp3, to mediate ERE-independent signaling. Ligand binding induces a conformational change allowing subsequent or combinatorial association with multiprotein coactivator complexes through LXXLL motifs of their respective components. Mutual transrepression occurs between the estrogen receptor (ER) and NF-kappa-B in a cell-type specific manner. Decreases NF-kappa-B DNA-binding activity and inhibits NF-kappa-B-mediated transcription from the IL6 promoter and displace RELA/p65 and associated coregulators from the promoter. Recruited to the NF-kappa-B response element of the CCL2 and IL8 promoters and can displace CREBBP. Present with NF-kappa-B components RELA/p65 and NFKB1/p50 on ERE sequences. Can also act synergistically with NF-kappa-B to activate transcription involving respective recruitment adjacent response elements; the function involves CREBBP. Can activate the transcriptional activity of TFF1. Also mediates membrane-initiated estrogen signaling involving various kinase cascades. Essential for MTA1-mediated transcriptional regulation of BRCA1 and BCAS3 (PubMed:17922032). {ECO:0000269|PubMed:10681512, ECO:0000269|PubMed:10816575, ECO:0000269|PubMed:11477071, ECO:0000269|PubMed:11682626, ECO:0000269|PubMed:14764652, ECO:0000269|PubMed:15078875, ECO:0000269|PubMed:15891768, ECO:0000269|PubMed:16043358, ECO:0000269|PubMed:16617102, ECO:0000269|PubMed:16684779, ECO:0000269|PubMed:17922032, ECO:0000269|PubMed:17932106, ECO:0000269|PubMed:18247370, ECO:0000269|PubMed:19350539, ECO:0000269|PubMed:20074560, ECO:0000269|PubMed:20705611, ECO:0000269|PubMed:21330404, ECO:0000269|PubMed:22083956, ECO:0000269|PubMed:7651415, ECO:0000269|PubMed:9328340}.; FUNCTION: [Isoform 3]: Involved in activation of NOS3 and endothelial nitric oxide production (PubMed:21937726). Isoforms lacking one or several functional domains are thought to modulate transcriptional activity by competitive ligand or DNA binding and/or heterodimerization with the full-length receptor (PubMed:10970861). Binds to ERE and inhibits isoform 1 (PubMed:10970861). {ECO:0000269|PubMed:10970861, ECO:0000269|PubMed:21937726}.
Subellular location
SUBCELLULAR LOCATION: [Isoform 1]: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407, ECO:0000269|PubMed:12682286, ECO:0000269|PubMed:20074560}. Cytoplasm {ECO:0000269|PubMed:12682286, ECO:0000269|PubMed:24498420}. Cell membrane {ECO:0000269|PubMed:12682286}; Peripheral membrane protein {ECO:0000269|PubMed:12682286}; Cytoplasmic side {ECO:0000269|PubMed:12682286}. Note=A minor fraction is associated with the inner membrane.; SUBCELLULAR LOCATION: [Isoform 3]: Nucleus. Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Single-pass type I membrane protein. Note=Associated with the inner membrane via palmitoylation (Probable). At least a subset exists as a transmembrane protein with a N-terminal extracellular domain. {ECO:0000305}.; SUBCELLULAR LOCATION: Nucleus. Golgi apparatus. Cell membrane. Note=Colocalizes with ZDHHC7 and ZDHHC21 in the Golgi apparatus where most probably palmitoylation occurs. Associated with the plasma membrane when palmitoylated.
Tissues
TISSUE SPECIFICITY: Widely expressed (PubMed:10970861). Not expressed in the pituitary gland (PubMed:10970861). {ECO:0000269|PubMed:10970861}.; TISSUE SPECIFICITY: [Isoform 3]: Widely expressed, however not expressed in the pituitary gland. {ECO:0000269|PubMed:10970861}.
Structure
SUBUNIT: Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with FOXC2, MAP1S, SLC30A9, UBE1C and NCOA3 coactivator (By similarity). Interacts with PELP1, the interaction is enhanced by 17-beta-estradiol, the interaction increases ESR1 transcriptional activity (PubMed:11481323, PubMed:14963108). Interacts with EP300; the interaction is estrogen-dependent and enhanced by CITED1. Interacts with CITED1; the interaction is estrogen-dependent. Interacts with NCOA5 and NCOA6 coactivators. Interacts with NCOA7; the interaction is a ligand-inducible. Interacts with PHB2, and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with KDM5A. Interacts with SMARD1. Interacts with HEXIM1. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ESR1-mediated transcription. Interacts with DNTTIP2, FAM120B and UIMC1. Interacts with isoform 4 of TXNRD1. Interacts with KMT2D/MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol. Interacts with KIF18A and LDB1. Interacts with RLIM (via C-terminus). Interacts with MACROD1. Interacts with SH2D4A and PLCG. Interaction with SH2D4A blocks binding to PLCG and inhibits estrogen-induced cell proliferation. Interacts with DYNLL1. Interacts with CCDC62 in the presence of estradiol/E2; this interaction seems to enhance the transcription of target genes. Interacts with NR2C1; the interaction prevents homodimerization of ESR1 and suppresses its transcriptional activity and cell growth. Interacts with DNAAF4. Interacts with PRMT2. Interacts with RBFOX2. Interacts with STK3/MST2 only in the presence of SAV1 and vice-versa. Binds to CSNK1D. Interacts with NCOA2; NCOA2 can interact with ESR1 AF-1 and AF-2 domains simultaneously and mediate their transcriptional synergy. Interacts with DDX5 (PubMed:11682626). Interacts with NCOA1; the interaction seems to require a self-association of N-terminal and C-terminal regions. Interacts with ZNF366, DDX17, NFKB1, RELA, SP1 and SP3. Interacts with NRIP1 (By similarity). Interacts with GPER1; the interaction occurs in an estrogen-dependent manner. Interacts with CLOCK and the interaction is stimulated by estrogen. Interacts with BCAS3. Interacts with TRIP4 (ufmylated); estrogen dependent. Interacts with LMTK3; the interaction phosphorylates ESR1 (in vitro) and protects it against proteasomal degradation. Interacts with CCAR2 (via N-terminus) in a ligand-independent manner. Interacts with ZFHX3. Interacts with SFR1 in a ligand-dependent and -independent manner (PubMed:23874500). Interacts with DCAF13, LATS1 and DCAF1; regulates ESR1 ubiquitination and ubiquitin-mediated proteasomal degradation (PubMed:28068668). Interacts (via DNA-binding domain) with POU4F2 (C-terminus); this interaction increases the estrogen receptor ESR1 transcriptional activity in a DNA- and ligand 17-beta-estradiol-independent manner (By similarity). Interacts with ESRRB isoform 1 (PubMed:19755138). Interacts with UBE3A and WBP2 (PubMed:16772533). Interacts with GTF2B (PubMed:1517211). Interacts with RBM39 (By similarity). In the absence of hormonal ligand, interacts with TACC1 (PubMed:20078863). Interacts with PI3KR1 or PI3KR2 and PTK2/FAK1 (PubMed:18657504). Interacts with SRC (PubMed:14963108, PubMed:18657504). {ECO:0000250, ECO:0000250|UniProtKB:P19785, ECO:0000269|PubMed:10359819, ECO:0000269|PubMed:10567404, ECO:0000269|PubMed:10681512, ECO:0000269|PubMed:10816575, ECO:0000269|PubMed:10970861, ECO:0000269|PubMed:11113208, ECO:0000269|PubMed:11265755, ECO:0000269|PubMed:11358960, ECO:0000269|PubMed:11477071, ECO:0000269|PubMed:11481323, ECO:0000269|PubMed:11581164, ECO:0000269|PubMed:11682626, ECO:0000269|PubMed:11875103, ECO:0000269|PubMed:11971969, ECO:0000269|PubMed:12039952, ECO:0000269|PubMed:12093804, ECO:0000269|PubMed:12554772, ECO:0000269|PubMed:12917342, ECO:0000269|PubMed:14963108, ECO:0000269|PubMed:15047147, ECO:0000269|PubMed:1517211, ECO:0000269|PubMed:15199063, ECO:0000269|PubMed:15891768, ECO:0000269|PubMed:15940264, ECO:0000269|PubMed:16427018, ECO:0000269|PubMed:16603732, ECO:0000269|PubMed:16772533, ECO:0000269|PubMed:17006958, ECO:0000269|PubMed:17043237, ECO:0000269|PubMed:17085477, ECO:0000269|PubMed:17311814, ECO:0000269|PubMed:17347654, ECO:0000269|PubMed:17505058, ECO:0000269|PubMed:17587566, ECO:0000269|PubMed:17658481, ECO:0000269|PubMed:17914104, ECO:0000269|PubMed:17932106, ECO:0000269|PubMed:17998543, ECO:0000269|PubMed:18563714, ECO:0000269|PubMed:18657504, ECO:0000269|PubMed:19117995, ECO:0000269|PubMed:19339517, ECO:0000269|PubMed:19350539, ECO:0000269|PubMed:19383985, ECO:0000269|PubMed:19423554, ECO:0000269|PubMed:19636373, ECO:0000269|PubMed:19712589, ECO:0000269|PubMed:19718048, ECO:0000269|PubMed:19749156, ECO:0000269|PubMed:19755138, ECO:0000269|PubMed:20074560, ECO:0000269|PubMed:20078863, ECO:0000269|PubMed:20720010, ECO:0000269|PubMed:21104395, ECO:0000269|PubMed:21602804, ECO:0000269|PubMed:23160374, ECO:0000269|PubMed:23874500, ECO:0000269|PubMed:25219498, ECO:0000269|PubMed:28068668, ECO:0000269|PubMed:9328340, ECO:0000269|PubMed:9627117}.; SUBUNIT: [Isoform 3]: Probably homodimerizes or heterodimerizes with isoform 1 and ESR2. {ECO:0000269|PubMed:10970861}.
Post-translational modification
PTM: Phosphorylated by cyclin A/CDK2 and CK1. Phosphorylation probably enhances transcriptional activity. Self-association induces phosphorylation. Dephosphorylation at Ser-118 by PPP5C inhibits its transactivation activity. Phosphorylated by LMTK3 in vitro. {ECO:0000269|PubMed:10428798, ECO:0000269|PubMed:14764652, ECO:0000269|PubMed:19339517, ECO:0000269|PubMed:21602804, ECO:0000269|PubMed:7476978, ECO:0000269|PubMed:7539106, ECO:0000269|PubMed:7838153}.; PTM: Glycosylated; contains N-acetylglucosamine, probably O-linked. {ECO:0000269|PubMed:8999954}.; PTM: Ubiquitinated; regulated by LATS1 via DCAF1 it leads to ESR1 proteasomal degradation (PubMed:21602804, PubMed:28068668). Deubiquitinated by OTUB1 (PubMed:19383985). {ECO:0000269|PubMed:19383985, ECO:0000269|PubMed:21602804, ECO:0000269|PubMed:28068668}.; PTM: Dimethylated by PRMT1 at Arg-260. The methylation may favor cytoplasmic localization (PubMed:18657504, PubMed:24498420). Demethylated by JMJD6 at Arg-260 (PubMed:24498420). {ECO:0000269|PubMed:18657504, ECO:0000269|PubMed:24498420}.; PTM: Palmitoylated (isoform 3). Not biotinylated (isoform 3). {ECO:0000269|PubMed:22031296}.; PTM: Palmitoylated by ZDHHC7 and ZDHHC21. Palmitoylation is required for plasma membrane targeting and for rapid intracellular signaling via ERK and AKT kinases and cAMP generation, but not for signaling mediated by the nuclear hormone receptor. {ECO:0000269|PubMed:22031296}.
Target Relevance information above includes information from UniProt accession : P03372
The UniProt Consortium

Data

benchmark-antibodies_anti-estrogen_receptoralpha_antibody_3616_1.jpg
Whole cell extract (60 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody (3616) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
benchmark-antibodies_anti-estrogen_receptoralpha_antibody_3616_2.jpg
Non-transfected (?) and transfected (+) 293T whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (3616) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
benchmark-antibodies_anti-estrogen_receptoralpha_antibody_3616_3.jpg
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Estrogen Receptor alpha antibody [1F3] (3616) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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Protocols

relevant to this product
Western blot
IHC

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