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mouse anti-DNA Ligase III monoclonal antibody (1F3) 4859

$503.00

Antibody summary

  • Mouse monoclonal to DNA Ligase III
  • Suitable for: WB,ICC/IF,IP,Blocking,PLA
  • Isotype: IgG1
  • 100 µg
SKU: 4859parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

DNA Ligase III

available sizes

100 µg

Available product – mouse anti-DNA Ligase III monoclonal antibody (1F3) 4859

antibody
Tested applications
WB,ICC/IF,IP
Recommended dilutions
Immunoblotting: use at 0.5-2ug/mL. Predicted molecular weight ~100kDa.

These are recommended concentrations.

Endusers should determine optimal concentrations for their applications.
Immunogen
Full-length recombinant human DNA Ligase III-b.
Size and concentration
100µg and 1 mg/mL
Form
liquid
Storage Instructions
This antibody is stable for at least one year at -20°C. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Storage buffer
Hybridoma culture supernatant
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
DNA ligase 3 (EC 6.5.1.1) (DNA ligase III) (Polydeoxyribonucleotide synthase [ATP] 3)
Gene names
LIG3,LIG3
Protein family
ATP-dependent DNA ligase family
Mass
112907Da
Function
FUNCTION: Isoform 3 functions as a heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as a DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627). {ECO:0000269|PubMed:10207110, ECO:0000269|PubMed:24674627}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.; EC=6.5.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10135, ECO:0000269|PubMed:10207110, ECO:0000269|PubMed:20518483};
Subellular location
SUBCELLULAR LOCATION: [Isoform 1]: Mitochondrion {ECO:0000269|PubMed:10207110, ECO:0000269|PubMed:24674627}. Note=Contains an N-terminal mitochondrial transit peptide. {ECO:0000269|PubMed:10207110}.; SUBCELLULAR LOCATION: [Isoform 2]: Mitochondrion {ECO:0000305|PubMed:10207110}. Note=Contains an N-terminal mitochondrial transit peptide. {ECO:0000305|PubMed:10207110}.; SUBCELLULAR LOCATION: [Isoform 3]: Nucleus {ECO:0000269|PubMed:10207110}. Note=Lacks the N-terminal mitochondrial transit peptide. {ECO:0000269|PubMed:10207110}.; SUBCELLULAR LOCATION: [Isoform 4]: Nucleus {ECO:0000305|PubMed:10207110}. Note=Lacks the N-terminal mitochondrial transit peptide. {ECO:0000305|PubMed:10207110}.
Tissues
TISSUE SPECIFICITY: Testis, thymus, prostate and heart.
Structure
SUBUNIT: Isoform 3 interacts (via BRCT domain) with the nuclear DNA-repair protein XRCC1. Interacts with POLG (PubMed:33855352). Interacts with POLB (PubMed:19336415). {ECO:0000269|PubMed:19336415, ECO:0000269|PubMed:21652643, ECO:0000269|PubMed:33855352, ECO:0000269|PubMed:7760816}.
Target Relevance information above includes information from UniProt accession : P49916
The UniProt Consortium

Data

benchmark-antibodies_anti-dna_ligase_iii_antibody_4859_1.jpg
DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Whole cell extracts (30 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (4859) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
benchmark-antibodies_anti-dna_ligase_iii_antibody_4859_2.jpg
DNA ligase III detects DNA ligase III antibody [1F3] protein by western blot analysis.
A. 30 µg 293T whole cell lysate/extract
B. 30 µg A431 whole cell lysate/extract
C. 30 µg HeLa whole cell lysate/extract
D. 30 µg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
DNA ligase III (4859) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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