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mouse anti-DNA Ligase I monoclonal antibody (10H5) 2446

$503.00

Antibody summary

  • Mouse monoclonal to DNA Ligase I
  • Suitable for: WB,ICC/IF,IHC-P,IP
  • Isotype: IgG1
  • 100 µg
SKU: 2446parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

DNA Ligase I

available sizes

100 µg

Available product – mouse anti-DNA Ligase I monoclonal antibody (10H5) 2446

antibody
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
Immunoblotting: use at 1-5 ug/mL.

Immunoprecipitation: use at 1-5 ug/mL.

Positive controls: MCF-7 cells or recombinant protein.
Immunogen
Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells.
Size and concentration
100µg and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles.
Storage buffer
PBS, pH 7.4
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
DNA ligase 1 (EC 6.5.1.1) (DNA ligase I) (Polydeoxyribonucleotide synthase [ATP] 1)
Gene names
LIG1,LIG1
Protein family
ATP-dependent DNA ligase family
Mass
101736Da
Function
FUNCTION: DNA ligase that seals nicks in double-stranded during DNA repair (PubMed:30395541). Also involved in DNA replication and DNA recombination. {ECO:0000269|PubMed:30395541}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.; EC=6.5.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10135};
Subellular location
SUBCELLULAR LOCATION: Nucleus.
Structure
SUBUNIT: Interacts with PCNA (PubMed:24911150). Interacts with POLB (PubMed:19336415). {ECO:0000269|PubMed:19336415, ECO:0000269|PubMed:24911150}.
Target Relevance information above includes information from UniProt accession : P18858
The UniProt Consortium

Data

benchmark-antibodies_anti-dna_ligase_i_antibody_2446_1.jpg
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] (2446) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
benchmark-antibodies_anti-dna_ligase_i_antibody_2446_2.jpg
DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA ligase I stained by DNA ligase I antibody [10H5] (2446) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 um.
benchmark-antibodies_anti-dna_ligase_i_antibody_2446_3.jpg
DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
DNA ligase I stained by DNA ligase I antibody [10H5] (2446) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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