| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | DNA Ligase I |
| available sizes | 100 µg |
mouse anti-DNA Ligase I monoclonal antibody (10H5) 2446
$503.00
Antibody summary
- Mouse monoclonal to DNA Ligase I
- Suitable for: WB,ICC/IF,IHC-P,IP
- Isotype: IgG1
- 100 µg
mouse anti-DNA Ligase I monoclonal antibody (10H5) 2446
| antibody |
|---|
| Tested applications WB,IHC,IHC,ICC/IF |
| Recommended dilutions Immunoblotting: use at 1-5 ug/mL. Immunoprecipitation: use at 1-5 ug/mL. Positive controls: MCF-7 cells or recombinant protein. |
| Immunogen Recombinant human DNA Ligase I (aa 1-919) purified from baculovirus infected Sf9 cells. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze- thaw cycles. |
| Storage buffer PBS, pH 7.4 |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names DNA ligase 1 (EC 6.5.1.1) (DNA ligase I) (Polydeoxyribonucleotide synthase [ATP] 1) |
| Gene names LIG1,LIG1 |
| Protein family ATP-dependent DNA ligase family |
| Mass 101736Da |
| Function FUNCTION: DNA ligase that seals nicks in double-stranded during DNA repair (PubMed:30395541). Also involved in DNA replication and DNA recombination. {ECO:0000269|PubMed:30395541}. |
| Catalytic activity CATALYTIC ACTIVITY: Reaction=ATP + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho-(deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + diphosphate.; EC=6.5.1.1; Evidence={ECO:0000255|PROSITE-ProRule:PRU10135}; |
| Subellular location SUBCELLULAR LOCATION: Nucleus. |
| Structure SUBUNIT: Interacts with PCNA (PubMed:24911150). Interacts with POLB (PubMed:19336415). {ECO:0000269|PubMed:19336415, ECO:0000269|PubMed:24911150}. |
| Target Relevance information above includes information from UniProt accession : P18858 |
| The UniProt Consortium |
Data
 |
| Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA ligase I antibody [10H5] (2446) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. |
 |
| DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DNA ligase I stained by DNA ligase I antibody [10H5] (2446) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 um. |
 |
| DNA ligase I antibody [10H5] detects DNA ligase I protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.DNA ligase I stained by DNA ligase I antibody [10H5] (2446) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC ICC |
Documents
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