| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Digoxigenin |
| available sizes | 100 µg |
mouse anti-Digoxigenin monoclonal antibody (21H8) 6611
$520.00
Antibody summary
- Mouse monoclonal to Digoxigenin
- Suitable for: WB,IHC,ELISA
- Isotype: IgG1
- 100 µg
mouse anti-Digoxigenin monoclonal antibody (21H8) 6611
| antibody |
|---|
| Tested applications WB,IHC,IHC,ELISA |
| Recommended dilutions ELISA: use at 0.1-1.0ug/ml Immunoblotting: User should use at 2-5ug/ml. Immunohistochemistry: Use at 1-5ug/ml. These are recommended concentrations. End user should determine optimal concentrations for their applications. |
| Immunogen Digoxigenin conjugate. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose |
| Purity protein affinity purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Digoxigenin |
| Alternative names DIG, Digoxigenine |
| Function Digoxigenin itself has no known physiological role in mammals. In research and diagnostic applications it serves as an antigenic label that can be detected with highly specific anti-digoxigenin antibodies. |
| Description Digoxigenin is a steroid hapten derived from the foxglove plant Digitalis. It is widely used as a non-radioactive labeling molecule for nucleic acids, proteins, peptides, and other biological reagents. |
| Chemical description Steroid hapten C23H34O5 390.52 Da Digitalis species |
| Biological role Research label Non-radioactive detection tag Immunodetection hapten |
| Research relevance Digoxigenin is one of the most widely used non-radioactive labeling systems in molecular biology. DIG-labeled probes provide sensitive detection of nucleic acids while avoiding the hazards associated with radioactive isotopes. |
| Keywords Hapten, Probe labeling, In situ hybridization, Nucleic acid detection, Anti-DIG antibody |
| Structure Digoxigenin is a steroid molecule derived from Digitalis species and is commonly used as a hapten label for biological molecules. |
| Function DIG serves as an antigenic tag that can be detected with highly specific anti-digoxigenin antibodies. |
| Applications DIG labeling is widely used for detection of DNA, RNA, oligonucleotides, proteins, and probes in molecular biology and diagnostic assays. |
| Detection DIG-labeled molecules are commonly detected using anti-DIG antibodies conjugated to alkaline phosphatase, horseradish peroxidase, fluorescent dyes, or other reporter systems. |
| Research relevance The DIG system is one of the most established non-radioactive alternatives to isotopic labeling and is widely used in hybridization and immunodetection assays. |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What are the recommended dilutions for using the mouse anti-Digoxigenin monoclonal antibody (21H8) in ELISA and immunohistochemistry?
For ELISA, it is recommended to use the antibody at a concentration of 0.1 to 1.0 µg/mL. For immunohistochemistry (IHC), use the antibody at 1 to 5 µg/mL. These concentrations serve as guidelines, and end users should optimize the dilution for their specific applications.
How should the mouse anti-Digoxigenin monoclonal antibody (21H8) be stored to maintain its stability?
The lyophilized antibody is stable for at least one year when stored at -20°C to -70°C. After reconstitution, it should be aliquoted appropriately and stored to avoid repeated freeze-thaw cycles to preserve its activity.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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