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mouse anti-Collagen V α-1 Chain (NC2 domain) monoclonal antibody (13F6) 7366

$520.00

Antibody summary

  • Mouse monoclonal to Collagen V α-1 Chain (NC2 domain)
  • Suitable for: WB,IHC
  • Isotype: IgG1
  • 100 µg
SKU: 7366parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Collagen Vα-1 Chain N-propeptide-NC2 domain

available sizes

100 µg

Available product – mouse anti-Collagen V α-1 Chain (NC2 domain) monoclonal antibody (13F6) 7366

antibody
Tested applications
WB,IHC,IHC
Recommended dilutions
Immunoblotting: use at 1-5ug/mL.

Immunohistochemistry: Formalin-fixed, paraffin-embedded tissue, heat-induced antigen retrieval. Use at 1-10ug/mL.

These are recommended concentrations.

Enduser should determine optimal concentrations for their applications.
Immunogen
Recombinant human collagen V alpha-1 chain N fragment.
Size and concentration
100µg and
Form
lyophilized
Storage Instructions
This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Storage buffer
Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
Collagen alpha-1(V) chain
Gene names
COL5A1,COL5A1
Protein family
Fibrillar collagen family
Mass
183560Da
Function
FUNCTION: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin.
Subellular location
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}.
Structure
SUBUNIT: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4. {ECO:0000269|PubMed:9099729}.
Post-translational modification
PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. {ECO:0000269|PubMed:2496661}.; PTM: Sulfated on 40% of tyrosines.
Target Relevance information above includes information from UniProt accession : P20908
The UniProt Consortium

Data

benchmark-antibodies_anti-collagen_valpha-1_chain_n-propeptide-nc2_doma
Immunohistochemistry: Formalin-fixed, paraffin-embedded tissue, heat-induced antigen retrieval. Use at 1-10ug/ml. Staining of human skin with #7366

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC

Documents

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