| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Collagen Vα-1 Chain N-propeptide-NC2 domain |
| available sizes | 100 µg |
mouse anti-Collagen V α-1 Chain (NC2 domain) monoclonal antibody (13F6) 7366
$520.00
Antibody summary
- Mouse monoclonal to Collagen V α-1 Chain (NC2 domain)
- Suitable for: WB,IHC
- Isotype: IgG1
- 100 µg
mouse anti-Collagen V α-1 Chain (NC2 domain) monoclonal antibody (13F6) 7366
| antibody |
|---|
| Tested applications WB,IHC,IHC |
| Recommended dilutions Immunoblotting: use at 1-5ug/mL. Immunohistochemistry: Formalin-fixed, paraffin-embedded tissue, heat-induced antigen retrieval. Use at 1-10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
| Immunogen Recombinant human collagen V alpha-1 chain N fragment. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names Collagen alpha-1(V) chain |
| Gene names COL5A1,COL5A1 |
| Protein family Fibrillar collagen family |
| Mass 183560Da |
| Function FUNCTION: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. |
| Subellular location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}. |
| Structure SUBUNIT: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4. {ECO:0000269|PubMed:9099729}. |
| Post-translational modification PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. {ECO:0000269|PubMed:2496661}.; PTM: Sulfated on 40% of tyrosines. |
| Target Relevance information above includes information from UniProt accession : P20908 |
| The UniProt Consortium |
Data
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| Immunohistochemistry: Formalin-fixed, paraffin-embedded tissue, heat-induced antigen retrieval. Use at 1-10ug/ml. Staining of human skin with #7366 |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC |
Documents
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