| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Collagen Vα-1 Chain N-propeptide-NC2 domain |
| available sizes | 100 µg |
mouse anti-Collagen V α-1 Chain (NC2 domain) monoclonal antibody (13F6) 7366
$520.00
Antibody summary
- Mouse monoclonal to Collagen V α-1 Chain (NC2 domain)
- Suitable for: WB,IHC
- Isotype: IgG1
- 100 µg
mouse anti-Collagen V α-1 Chain (NC2 domain) monoclonal antibody (13F6) 7366
| antibody |
|---|
| Tested applications WB,IHC,IHC |
| Recommended dilutions Immunoblotting: use at 1-5ug/mL. Immunohistochemistry: Formalin-fixed, paraffin-embedded tissue, heat-induced antigen retrieval. Use at 1-10ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
| Immunogen Recombinant human collagen V alpha-1 chain N fragment. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose |
| Purity protein affinity purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names Collagen alpha-1(V) chain |
| Gene names COL5A1,COL5A1 |
| Protein family Fibrillar collagen family |
| Mass 183560Da |
| Function FUNCTION: Type V collagen is a member of group I collagen (fibrillar forming collagen). It is a minor connective tissue component of nearly ubiquitous distribution. Type V collagen binds to DNA, heparan sulfate, thrombospondin, heparin, and insulin. |
| Subellular location SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix {ECO:0000255|PROSITE-ProRule:PRU00793}. |
| Structure SUBUNIT: Trimers of two alpha 1(V) and one alpha 2(V) chains in most tissues and trimers of one alpha 1(V), one alpha 2(V), and one alpha 3(V) chains in placenta. Interacts with CSPG4. {ECO:0000269|PubMed:9099729}. |
| Post-translational modification PTM: Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains. {ECO:0000269|PubMed:2496661}.; PTM: Sulfated on 40% of tyrosines. |
| Domain DOMAIN: The C-terminal propeptide, also known as COLFI domain, have crucial roles in tissue growth and repair by controlling both the intracellular assembly of procollagen molecules and the extracellular assembly of collagen fibrils. It binds a calcium ion which is essential for its function (By similarity). {ECO:0000250}. |
| Involvement in disease DISEASE: Ehlers-Danlos syndrome, classic type, 1 (EDSCL1) [MIM:130000]: A form of Ehlers-Danlos syndrome, a group of connective tissue disorders characterized by skin hyperextensibility, articular hypermobility, and tissue fragility. The main features of classic Ehlers-Danlos syndrome are joint hypermobility and dislocation, and fragile, bruisable skin. EDSCL1 inheritance is autosomal dominant. {ECO:0000269|PubMed:10602121, ECO:0000269|PubMed:11992482, ECO:0000269|PubMed:15580559, ECO:0000269|PubMed:18972565, ECO:0000269|PubMed:9042913}. Note=The disease is caused by variants affecting the gene represented in this entry.; DISEASE: Fibromuscular dysplasia, multifocal (FMDMF) [MIM:619329]: An autosomal dominant vascular disorder with incomplete penetrance, characterized by fibrous tissue and webs developing in the artery wall and leading to multiple arterial stenoses. Patients with multifocal fibromuscular dysplasia can develop arterial tortuosity, macroaneurysms, and dissections. Arterial rupture may occur. {ECO:0000269|PubMed:32938213}. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: P20908 |
| The UniProt Consortium |
Data
 |
| Immunohistochemistry: Formalin-fixed, paraffin-embedded tissue, heat-induced antigen retrieval. Use at 1-10ug/ml. Staining of human skin with #7366 |
FAQ & Publications
Frequently Asked Questions
What applications has the mouse anti-Collagen V α-1 Chain (NC2 domain) monoclonal antibody (13F6) been validated for?
This antibody has been tested and is suitable for use in Western blotting (WB) and immunohistochemistry (IHC) applications.
How should the mouse anti-Collagen V α-1 Chain (NC2 domain) monoclonal antibody (13F6) be stored to maintain its stability?
The lyophilized antibody is stable for at least one year when stored at -20°C to -70°C. After reconstitution, it should be aliquoted and stored to avoid repeated freeze-thaw cycles.
What is the recommended dilution range for immunohistochemistry using this antibody on formalin-fixed, paraffin-embedded tissue?
For IHC with formalin-fixed, paraffin-embedded tissue and heat-induced antigen retrieval, the recommended dilution range is 1-10 µg/mL, although users should optimize concentrations for their specific applications.
What is the host species and isotype of this monoclonal antibody?
The antibody is a mouse monoclonal of the IgG1 isotype.
What is the immunogen used to generate the mouse anti-Collagen V α-1 Chain (NC2 domain) monoclonal antibody (13F6)?
The antibody was raised against a recombinant human collagen V alpha-1 chain N fragment.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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