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mouse anti-CD73/5′-Nucleotidase monoclonal antibody (10f1) 2858

$520.00

Antibody summary

  • Mouse monoclonal to CD73/5′-Nucleotidase
  • Suitable for: WB,IHC,ELISA
  • Isotype: IgG1
  • 100 µg
SKU: 2858parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

CD73/5'Nucleotidase

available sizes

100 µg

Available product – mouse anti-CD73/5′-Nucleotidase monoclonal antibody (10f1) 2858

antibody
Tested applications
WB,IHC,IHC,ELISA
Recommended dilutions
ELISA: use at 1-10ug/ml

Immunoblotting:

User should use at 1-5ug/ml. A band of ~63kDa is detected.

Immunohistochemistry: use at 1-5ug/ml.

These are recommended concentrations.

End user should determine optimal concentrations for their applications.
Immunogen
Recombinant 5'-nucleotidase (5'-NT). Accession no. P21589.
Size and concentration
100µg and
Form
lyophilized
Storage Instructions
This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles.
Storage buffer
Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose
Purity
protein affinty purification
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
5'-nucleotidase (5'-NT) (EC 3.1.3.35) (EC 3.1.3.5) (EC 3.1.3.89) (EC 3.1.3.91) (EC 3.1.3.99) (5'-deoxynucleotidase) (Ecto-5'-nucleotidase) (IMP-specific 5'-nucleotidase) (Thymidylate 5'-phosphatase) (CD antigen CD73)
Gene names
NT5E,NT5E NT5 NTE
Protein family
5'-nucleotidase family
Mass
63368Da
Function
FUNCTION: Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). {ECO:0000269|PubMed:21933152, ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:23142347, ECO:0000269|PubMed:24887587, ECO:0000269|PubMed:34403084}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377, ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5; Evidence={ECO:0000269|PubMed:21933152, ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:23142347, ECO:0000269|PubMed:24887587, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate; Xref=Rhea:RHEA:36167, ChEBI:CHEBI:15377, ChEBI:CHEBI:18274, ChEBI:CHEBI:43474, ChEBI:CHEBI:65317; EC=3.1.3.89; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=dTMP + H2O = phosphate + thymidine; Xref=Rhea:RHEA:11080, ChEBI:CHEBI:15377, ChEBI:CHEBI:17748, ChEBI:CHEBI:43474, ChEBI:CHEBI:63528; EC=3.1.3.35; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=CMP + H2O = cytidine + phosphate; Xref=Rhea:RHEA:29367, ChEBI:CHEBI:15377, ChEBI:CHEBI:17562, ChEBI:CHEBI:43474, ChEBI:CHEBI:60377; EC=3.1.3.91; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=H2O + IMP = inosine + phosphate; Xref=Rhea:RHEA:27718, ChEBI:CHEBI:15377, ChEBI:CHEBI:17596, ChEBI:CHEBI:43474, ChEBI:CHEBI:58053; EC=3.1.3.99; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=AMP + H2O = adenosine + phosphate; Xref=Rhea:RHEA:29375, ChEBI:CHEBI:15377, ChEBI:CHEBI:16335, ChEBI:CHEBI:43474, ChEBI:CHEBI:456215; Evidence={ECO:0000269|PubMed:21933152, ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:23142347, ECO:0000269|PubMed:24887587, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=GMP + H2O = guanosine + phosphate; Xref=Rhea:RHEA:27714, ChEBI:CHEBI:15377, ChEBI:CHEBI:16750, ChEBI:CHEBI:43474, ChEBI:CHEBI:58115; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=H2O + UMP = phosphate + uridine; Xref=Rhea:RHEA:29359, ChEBI:CHEBI:15377, ChEBI:CHEBI:16704, ChEBI:CHEBI:43474, ChEBI:CHEBI:57865; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=dAMP + H2O = 2'-deoxyadenosine + phosphate; Xref=Rhea:RHEA:29371, ChEBI:CHEBI:15377, ChEBI:CHEBI:17256, ChEBI:CHEBI:43474, ChEBI:CHEBI:58245; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=dCMP + H2O = 2'-deoxycytidine + phosphate; Xref=Rhea:RHEA:29363, ChEBI:CHEBI:15377, ChEBI:CHEBI:15698, ChEBI:CHEBI:43474, ChEBI:CHEBI:57566; Evidence={ECO:0000269|PubMed:34403084};
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:2129526, ECO:0000269|PubMed:24887587}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:2129526}.
Structure
SUBUNIT: Homodimer. {ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:23142347}.
Target Relevance information above includes information from UniProt accession : P21589
The UniProt Consortium

Data

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Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

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Western blot
IHC

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