| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | CD73/5'Nucleotidase |
| available sizes | 100 µg |
mouse anti-CD73/5′-Nucleotidase monoclonal antibody (10f1) 2858
$520.00
Antibody summary
- Mouse monoclonal to CD73/5′-Nucleotidase
- Suitable for: WB,IHC,ELISA
- Isotype: IgG1
- 100 µg
mouse anti-CD73/5′-Nucleotidase monoclonal antibody (10f1) 2858
| antibody |
|---|
| Tested applications WB,IHC,IHC,ELISA |
| Recommended dilutions ELISA: use at 1-10ug/ml Immunoblotting: User should use at 1-5ug/ml. A band of ~63kDa is detected. Immunohistochemistry: use at 1-5ug/ml. These are recommended concentrations. End user should determine optimal concentrations for their applications. |
| Immunogen Recombinant 5'-nucleotidase (5'-NT). Accession no. P21589. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names 5'-nucleotidase (5'-NT) (EC 3.1.3.35) (EC 3.1.3.5) (EC 3.1.3.89) (EC 3.1.3.91) (EC 3.1.3.99) (5'-deoxynucleotidase) (Ecto-5'-nucleotidase) (IMP-specific 5'-nucleotidase) (Thymidylate 5'-phosphatase) (CD antigen CD73) |
| Gene names NT5E,NT5E NT5 NTE |
| Protein family 5'-nucleotidase family |
| Mass 63368Da |
| Function FUNCTION: Catalyzes the hydrolysis of nucleotide monophosphates, releasing inorganic phosphate and the corresponding nucleoside, with AMP being the preferred substrate (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). Shows a preference for ribonucleotide monophosphates over their equivalent deoxyribose forms (PubMed:34403084). Other substrates include IMP, UMP, GMP, CMP, dAMP, dCMP, dTMP, NAD and NMN (PubMed:21933152, PubMed:34403084, PubMed:24887587, PubMed:22997138, PubMed:23142347). {ECO:0000269|PubMed:21933152, ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:23142347, ECO:0000269|PubMed:24887587, ECO:0000269|PubMed:34403084}. |
| Catalytic activity CATALYTIC ACTIVITY: Reaction=a ribonucleoside 5'-phosphate + H2O = a ribonucleoside + phosphate; Xref=Rhea:RHEA:12484, ChEBI:CHEBI:15377, ChEBI:CHEBI:18254, ChEBI:CHEBI:43474, ChEBI:CHEBI:58043; EC=3.1.3.5; Evidence={ECO:0000269|PubMed:21933152, ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:23142347, ECO:0000269|PubMed:24887587, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=a 2'-deoxyribonucleoside 5'-phosphate + H2O = a 2'-deoxyribonucleoside + phosphate; Xref=Rhea:RHEA:36167, ChEBI:CHEBI:15377, ChEBI:CHEBI:18274, ChEBI:CHEBI:43474, ChEBI:CHEBI:65317; EC=3.1.3.89; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=dTMP + H2O = phosphate + thymidine; Xref=Rhea:RHEA:11080, ChEBI:CHEBI:15377, ChEBI:CHEBI:17748, ChEBI:CHEBI:43474, ChEBI:CHEBI:63528; EC=3.1.3.35; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=CMP + H2O = cytidine + phosphate; Xref=Rhea:RHEA:29367, ChEBI:CHEBI:15377, ChEBI:CHEBI:17562, ChEBI:CHEBI:43474, ChEBI:CHEBI:60377; EC=3.1.3.91; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=H2O + IMP = inosine + phosphate; Xref=Rhea:RHEA:27718, ChEBI:CHEBI:15377, ChEBI:CHEBI:17596, ChEBI:CHEBI:43474, ChEBI:CHEBI:58053; EC=3.1.3.99; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=AMP + H2O = adenosine + phosphate; Xref=Rhea:RHEA:29375, ChEBI:CHEBI:15377, ChEBI:CHEBI:16335, ChEBI:CHEBI:43474, ChEBI:CHEBI:456215; Evidence={ECO:0000269|PubMed:21933152, ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:23142347, ECO:0000269|PubMed:24887587, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=GMP + H2O = guanosine + phosphate; Xref=Rhea:RHEA:27714, ChEBI:CHEBI:15377, ChEBI:CHEBI:16750, ChEBI:CHEBI:43474, ChEBI:CHEBI:58115; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=H2O + UMP = phosphate + uridine; Xref=Rhea:RHEA:29359, ChEBI:CHEBI:15377, ChEBI:CHEBI:16704, ChEBI:CHEBI:43474, ChEBI:CHEBI:57865; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=dAMP + H2O = 2'-deoxyadenosine + phosphate; Xref=Rhea:RHEA:29371, ChEBI:CHEBI:15377, ChEBI:CHEBI:17256, ChEBI:CHEBI:43474, ChEBI:CHEBI:58245; Evidence={ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:34403084}; CATALYTIC ACTIVITY: Reaction=dCMP + H2O = 2'-deoxycytidine + phosphate; Xref=Rhea:RHEA:29363, ChEBI:CHEBI:15377, ChEBI:CHEBI:15698, ChEBI:CHEBI:43474, ChEBI:CHEBI:57566; Evidence={ECO:0000269|PubMed:34403084}; |
| Subellular location SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:2129526, ECO:0000269|PubMed:24887587}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:2129526}. |
| Structure SUBUNIT: Homodimer. {ECO:0000269|PubMed:22997138, ECO:0000269|PubMed:23142347}. |
| Target Relevance information above includes information from UniProt accession : P21589 |
| The UniProt Consortium |
Data
| No results found |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
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| Western blot IHC |
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