mouse anti-Calreticulin monoclonal antibody (6C6) 1726

$100.00 – $2,600.00

Antibody summary

Mouse monoclonal to Calreticulin
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG1
100 µg, 25 µg, 1 mg

SKU: 1726parent Categories: antibody, cell marker Tag: Calreticulin

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

mouse anti-Calreticulin monoclonal antibody (6C6) 1726

antibody
Database link: human P27797 mouse P14211 rat P18418
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:1000-1:2000 IF/ICC and IHC: 1:1000
Immunogen Synthetic peptides VESGSLEDDWDFLPPKKI corresponding to amino acids 191-208 of human calreticulin, including the LC3 interacting region or LIR.
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monocolonal IgG1 - Isotype Control

target relevance
Calreticulin, an abundant protein found in the endoplasmic reticulum (ER), serves both as an essential organelle marker and a cell marker. Within the ER, calreticulin plays a critical role in calcium homeostasis and protein folding, ensuring proper maturation and quality control of newly synthesized proteins. Its presence and function in the ER make calreticulin a valuable organelle marker, aiding in the identification and localization of this dynamic and essential cellular compartment. Additionally, calreticulin has emerged as a prominent cell marker due to its distinct expression patterns in various cell types and physiological conditions. By detecting and measuring the levels of calreticulin on the cell surface, researchers can identify and isolate specific cell populations, such as stem cells or cancer cells. The ability to use calreticulin as both an organelle and cell marker offers valuable insights into cellular processes and opens up new avenues for studying cell biology, disease mechanisms, and developing targeted therapies. Click for more on: cell markers and Calreticulin
Protein names Calreticulin (CRP55) (Calregulin) (Endoplasmic reticulum resident protein 60) (ERp60) (HACBP) (grp60)
Gene names CALR,CALR CRTC
Protein family Calreticulin family
Mass 48142Da
Function Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity).
Subellular location Endoplasmic reticulum lumen Cytoplasm, cytosol Secreted, extracellular space, extracellular matrix Cell surface Sarcoplasmic reticulum lumen Cytoplasmic vesicle, secretory vesicle, Cortical granule Cytolytic granule Note=Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). During oocyte maturation and after parthenogenetic activation accumulates in cortical granules. In pronuclear and early cleaved embryos localizes weakly to cytoplasm around nucleus and more strongly in the region near the cortex (By similarity). In cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation (By similarity).
Structure Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 and SPACA9 (By similarity). Interacts with TRIM21 (PubMed:8666824). Interacts with NR3C1 (PubMed:11149926). Interacts with PPIB (PubMed:20801878). Interacts (via P-domain) with PDIA5 (PubMed:23614004). Interacts with GABARAP (PubMed:19154346). Interacts with HLA-E-B2M and HLA-G-B2M complexes (PubMed:9427624, PubMed:9640257). Interacts with HLA-F (PubMed:10605026). Interacts with CLCC1 (PubMed:30157172).
Involvement in disease Note=CALR somatic mutations are frequently found in myeloproliferative neoplasms lacking JAK2 or MPL mutations. Myeloproliferative neoplasms are chronic myeloid cancers characterized by overproduction of mature blood cells, and may evolve into acute myeloid leukemia. In addition to chronic myeloid leukemia with the BCR-ABL fusion gene, the three most common myeloproliferative neoplasms are essential thrombocythemia, polycythemia vera, and myelofibrosis.
Target Relevance information above includes information from UniProt accession: P27797
The UniProt Consortium

Data

Chromogenic immunostaining of a formalin fixed paraffin embedded human brain cortex section with mouse mAb to calreticulin, 1726, dilution 1:500, detected with DAB (brown) using the Vector Labs ImmPRESS method and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In this image, 1726 intensely labels the cytoplasm of pyramidal neurons. This antibody performs well in testing with both 4% PFA and standard NBF fixed tissues from human and rat.

Western blot analysis of lysates of different cell lines using mouse mAb to calreticulin, 1726, dilution 1:2,000: [1] protein standard, [2] NIH-3T3, [3] HEK293, [4] HeLa, [5] SH-SY5Y, [6] C6, [7] COS-1 cells. A strong single band at about 50kDa corresponds to the calreticulin protein.

Immunofluorescent analysis of of SH-SY5Y cells stained with mouse mAb to calreticulin, MCA-6C6, dilution 1:500 in green and costained with chicken pAb to lamin A/C, CPCA-LaminAC, dilution 1:2,000 in red. The blue is DAPI staining of nuclear DNA. The MCA-6C6 antibody reveals granular staining of cytoplasm, while the lamin A/C antibody stains the nuclear lamina and membrane.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 12 publications in our database for this antibody or clone. Here are the latest 5, for more click below.

pmid	title	authors	citation
35752171	Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1	Killackey SA, Bi Y, Soares F, Hammi I, Winsor NJ, Abdul-Sater AA, Philpott DJ, Arnoult D, Girardin SE.	Mol Cell. 2022 Aug 4;82(15):2815-2831.e5. doi: 10.1016/j.molcel.2022.06.004. Epub 2022 Jun 24.
30429217	The ER-localized Ca(2+)-binding protein calreticulin couples ER stress to autophagy by associating with microtubule-associated protein 1A/1B light chain 3	Yang Y, Ma F, Liu Z, Su Q, Liu Y, Liu Z, Li Y.	J Biol Chem. 2019 Jan 18;294(3):772-782. doi: 10.1074/jbc.RA118.005166. Epub 2018 Nov 14.
29175313	Relevance of the chaperone-like protein calreticulin for the biological behavior and clinical outcome of cancer	Fucikova J, Kasikova L, Truxova I, Laco J, Skapa P, Ryska A, Spisek R.	Immunol Lett. 2018 Jan;193:25-34. doi: 10.1016/j.imlet.2017.11.006. Epub 2017 Nov 23.
24070471	The Atg8 family: multifunctional ubiquitin-like key regulators of autophagy	Slobodkin MR, Elazar Z.	Essays Biochem. 2013;55:51-64. doi: 10.1042/bse0550051.
23908376	The LIR motif - crucial for selective autophagy	Birgisdottir ÅB, Lamark T, Johansen T.	J Cell Sci. 2013 Aug 1;126(Pt 15):3237-47. doi: 10.1242/jcs.126128.