Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
host | mouse |
isotype | IgG1 |
clonality | monoclonal |
concentration | 1 mg/mL |
applications | ICC/IF, IHC, WB |
reactivity | human, mouse, rat |
available sizes | 1 mg, 100 µg, 25 µg |
mouse anti-Calreticulin monoclonal antibody (6C6) 1726
$100.00 – $2,600.00
Antibody summary
- Mouse monoclonal to Calreticulin
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgG1
- 100 µg, 25 µg, 1 mg
mouse anti-Calreticulin monoclonal antibody (6C6) 1726
antibody |
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Database link: human P27797 mouse P14211 rat P18418 |
Tested applications WB,IHC,IHC,ICC/IF |
Recommended dilutions WB: 1:1000-1:2000 IF/ICC and IHC: 1:1000 |
Immunogen Synthetic peptides VESGSLEDDWDFLPPKKI corresponding to amino acids 191-208 of human calreticulin, including the LC3 interacting region or LIR. |
Size and concentration 25, 100, 1000µg and 1 mg/mL |
Form liquid |
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
Storage buffer PBS, 50% glycerol, 0.04% NaN3 |
Purity affinity purified |
Clonality monoclonal |
Isotype IgG1 |
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
Isotype control Mouse monocolonal IgG1 - Isotype Control |
target relevance |
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Calreticulin, an abundant protein found in the endoplasmic reticulum (ER), serves both as an essential organelle marker and a cell marker. Within the ER, calreticulin plays a critical role in calcium homeostasis and protein folding, ensuring proper maturation and quality control of newly synthesized proteins. Its presence and function in the ER make calreticulin a valuable organelle marker, aiding in the identification and localization of this dynamic and essential cellular compartment. Additionally, calreticulin has emerged as a prominent cell marker due to its distinct expression patterns in various cell types and physiological conditions. By detecting and measuring the levels of calreticulin on the cell surface, researchers can identify and isolate specific cell populations, such as stem cells or cancer cells. The ability to use calreticulin as both an organelle and cell marker offers valuable insights into cellular processes and opens up new avenues for studying cell biology, disease mechanisms, and developing targeted therapies. Click for more on: cell markers and Calreticulin |
Protein names Calreticulin (CRP55) (Calregulin) (Endoplasmic reticulum resident protein 60) (ERp60) (HACBP) (grp60) |
Gene names CALR,CALR CRTC |
Protein family Calreticulin family |
Mass 48142Da |
Function FUNCTION: Calcium-binding chaperone that promotes folding, oligomeric assembly and quality control in the endoplasmic reticulum (ER) via the calreticulin/calnexin cycle. This lectin interacts transiently with almost all of the monoglucosylated glycoproteins that are synthesized in the ER (PubMed:7876246). Interacts with the DNA-binding domain of NR3C1 and mediates its nuclear export (PubMed:11149926). Involved in maternal gene expression regulation. May participate in oocyte maturation via the regulation of calcium homeostasis (By similarity). Present in the cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation and might participate in the block to polyspermy (By similarity). {ECO:0000250|UniProtKB:P28491, ECO:0000250|UniProtKB:Q8K3H7, ECO:0000269|PubMed:11149926, ECO:0000269|PubMed:7876246}. |
Subellular location SUBCELLULAR LOCATION: Endoplasmic reticulum lumen {ECO:0000269|PubMed:10358038, ECO:0000269|PubMed:11149926}. Cytoplasm, cytosol {ECO:0000269|PubMed:11149926}. Secreted, extracellular space, extracellular matrix {ECO:0000305}. Cell surface {ECO:0000269|PubMed:10358038}. Sarcoplasmic reticulum lumen {ECO:0000250|UniProtKB:P28491}. Cytoplasmic vesicle, secretory vesicle, Cortical granule {ECO:0000250|UniProtKB:Q8K3H7}. Cytolytic granule {ECO:0000269|PubMed:8418194}. Note=Also found in cell surface (T cells), cytosol and extracellular matrix (PubMed:10358038). During oocyte maturation and after parthenogenetic activation accumulates in cortical granules. In pronuclear and early cleaved embryos localizes weakly to cytoplasm around nucleus and more strongly in the region near the cortex (By similarity). In cortical granules of non-activated oocytes, is exocytosed during the cortical reaction in response to oocyte activation (By similarity). {ECO:0000250|UniProtKB:P28491, ECO:0000250|UniProtKB:Q8K3H7, ECO:0000269|PubMed:8418194}. |
Structure SUBUNIT: Monomer. Component of an EIF2 complex at least composed of CELF1/CUGBP1, CALR, CALR3, EIF2S1, EIF2S2, HSP90B1 and HSPA5. Interacts with PDIA3/ERp57 and SPACA9 (By similarity). Interacts with TRIM21 (PubMed:8666824). Interacts with NR3C1 (PubMed:11149926). Interacts with PPIB (PubMed:20801878). Interacts (via P-domain) with PDIA5 (PubMed:23614004). Interacts with GABARAP (PubMed:19154346). Interacts with HLA-E-B2M and HLA-G-B2M complexes (PubMed:9427624, PubMed:9640257). Interacts with HLA-F (PubMed:10605026). Interacts with CLCC1 (PubMed:30157172). {ECO:0000250|UniProtKB:P14211, ECO:0000250|UniProtKB:P18418, ECO:0000269|PubMed:10605026, ECO:0000269|PubMed:11149926, ECO:0000269|PubMed:19154346, ECO:0000269|PubMed:20801878, ECO:0000269|PubMed:23614004, ECO:0000269|PubMed:30157172, ECO:0000269|PubMed:8666824, ECO:0000269|PubMed:9427624, ECO:0000269|PubMed:9640257}. |
Domain DOMAIN: Can be divided into a N-terminal globular domain, a proline-rich P-domain forming an elongated arm-like structure and a C-terminal acidic domain. The P-domain binds one molecule of calcium with high affinity, whereas the acidic C-domain binds multiple calcium ions with low affinity.; DOMAIN: The interaction with glycans occurs through a binding site in the globular lectin domain.; DOMAIN: The zinc binding sites are localized to the N-domain.; DOMAIN: Associates with PDIA3 through the tip of the extended arm formed by the P-domain. |
Involvement in disease DISEASE: Note=CALR somatic mutations are frequently found in myeloproliferative neoplasms lacking JAK2 or MPL mutations. Myeloproliferative neoplasms are chronic myeloid cancers characterized by overproduction of mature blood cells, and may evolve into acute myeloid leukemia. In addition to chronic myeloid leukemia with the BCR-ABL fusion gene, the three most common myeloproliferative neoplasms are essential thrombocythemia, polycythemia vera, and myelofibrosis. {ECO:0000269|PubMed:24325356, ECO:0000269|PubMed:24325359}. |
Target Relevance information above includes information from UniProt accession: P27797 |
The UniProt Consortium |
Data
Publications
Publications
pmid | title | authors | citation |
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35752171 | Mitochondrial protein import stress regulates the LC3 lipidation step of mitophagy through NLRX1 and RRBP1. | Samuel A Killackey, Yuntian Bi, Fraser Soares, Ikram Hammi, Nathaniel J Winsor, Ali A Abdul-Sater, Dana J Philpott, Damien Arnoult, Stephen E Girardin | Mol Cell 82:2815-2831.e5 |
30429217 | The ER-localized Ca(2+)-binding protein calreticulin couples ER stress to autophagy by associating with microtubule-associated protein 1A/1B light chain 3. | Yunzhi Yang, Fengguang Ma, Zhengshuai Liu, Qian Su, Yuxiao Liu, Zhixue Liu, Yu Li | J Biol Chem 294:772-782 |
29175313 | Relevance of the chaperone-like protein calreticulin for the biological behavior and clinical outcome of cancer. | Jitka Fucikova, Lenka Kasikova, Iva Truxova, Jan Laco, Petr Skapa, Ales Ryska, Radek Spisek | Immunol Lett 193:25-34 |
24070471 | The Atg8 family: multifunctional ubiquitin-like key regulators of autophagy. | Moran Rawet Slobodkin, Zvulun Elazar | Essays Biochem 55:51-64 |
23908376 | The LIR motif - crucial for selective autophagy. | Åsa Birna Birgisdottir, Trond Lamark, Terje Johansen | J Cell Sci 126:3237-47 |
Protocols
relevant to this product |
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Western blot IHC ICC |
Documents
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