| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Calnexin |
| available sizes | 100 µg |
mouse anti-Calnexin monoclonal antibody (C2A1) 9154
$520.00
Antibody summary
- Mouse monoclonal to Calnexin
- Suitable for: WB,IHC
- Isotype: IgG1
- 100 µg
mouse anti-Calnexin monoclonal antibody (C2A1) 9154
| antibody |
|---|
| Tested applications WB,IHC,IHC |
| Recommended dilutions Immunoblotting: use at 1-10ug/ml. Immunohistochemistry: use at 1-10ug/ml. These are recommended concentrations. End user should determine optimal concentrations for their applications. |
| Immunogen Dictyostelium discoideum AX2 recombinant fragment. Accession no. P27824. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucrose |
| Purity protein affinty purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names Calnexin (IP90) (Major histocompatibility complex class I antigen-binding protein p88) (p90) |
| Gene names CANX,CANX |
| Protein family Calreticulin family |
| Mass 67568Da |
| Function FUNCTION: Calcium-binding protein that interacts with newly synthesized monoglucosylated glycoproteins in the endoplasmic reticulum. It may act in assisting protein assembly and/or in the retention within the ER of unassembled protein subunits. It seems to play a major role in the quality control apparatus of the ER by the retention of incorrectly folded proteins. Associated with partial T-cell antigen receptor complexes that escape the ER of immature thymocytes, it may function as a signaling complex regulating thymocyte maturation. Additionally it may play a role in receptor-mediated endocytosis at the synapse. |
| Subellular location SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000269|PubMed:22314232}; Single-pass type I membrane protein {ECO:0000255}. Mitochondrion membrane {ECO:0000250|UniProtKB:P24643}; Single-pass type I membrane protein {ECO:0000255}. Melanosome membrane {ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065}; Single-pass type I membrane protein {ECO:0000255}. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545, PubMed:17081065). The palmitoylated form preferentially localizes to the perinuclear rough ER (PubMed:22314232). Localizes to endoplasmic reticulum mitochondria-associated membrane (MAMs) that connect the endoplasmic reticulum and the mitochondria (By similarity). {ECO:0000250|UniProtKB:P24643, ECO:0000269|PubMed:12643545, ECO:0000269|PubMed:17081065, ECO:0000269|PubMed:22314232}. |
| Structure SUBUNIT: Interacts with MAPK3/ERK1 (By similarity). Interacts with KCNH2 (PubMed:16361248). Associates with ribosomes (By similarity). Interacts with SGIP1; involved in negative regulation of endocytosis (By similarity). The palmitoylated form interacts with the ribosome-translocon complex component SSR1, promoting efficient folding of glycoproteins (PubMed:22314232). Interacts with SERPINA2P/SERPINA2 and with the S and Z variants of SERPINA1 (PubMed:23826168). Interacts with PPIB (PubMed:20801878). Interacts with ZNRF4 (PubMed:21205830). Interacts with SMIM22 (PubMed:29765154). Interacts with TMX2 (PubMed:31735293). Interacts with TMEM35A/NACHO (By similarity). Interacts with CHRNA7 (PubMed:32783947). Interacts with reticulophagy regulators RETREG2 and RETREG3 (PubMed:34338405). Interacts with DNM1L; may form part of a larger protein complex at the ER-mitochondrial interface during mitochondrial fission (PubMed:24196833). Interacts with ADAM7 (By similarity). {ECO:0000250|UniProtKB:P35564, ECO:0000250|UniProtKB:P35565, ECO:0000269|PubMed:16361248, ECO:0000269|PubMed:20801878, ECO:0000269|PubMed:21205830, ECO:0000269|PubMed:22314232, ECO:0000269|PubMed:23826168, ECO:0000269|PubMed:29765154, ECO:0000269|PubMed:31735293, ECO:0000269|PubMed:32783947, ECO:0000269|PubMed:34338405}.; SUBUNIT: (Microbial infection) Interacts with HBV large envelope protein, isoform L. {ECO:0000269|PubMed:9188622}.; SUBUNIT: (Microbial infection) Interacts with HBV large envelope protein, isoform M; this association may be essential for isoform M proper secretion. {ECO:0000269|PubMed:9188622}. |
| Post-translational modification PTM: Phosphorylated at Ser-564 by MAPK3/ERK1. Phosphorylation by MAPK3/ERK1 increases its association with ribosomes (By similarity). {ECO:0000250}.; PTM: Palmitoylation by DHHC6 leads to the preferential localization to the perinuclear rough ER. It mediates the association of calnexin with the ribosome-translocon complex (RTC) which is required for efficient folding of glycosylated proteins. {ECO:0000269|PubMed:22314232}.; PTM: Ubiquitinated, leading to proteasomal degradation. Probably ubiquitinated by ZNRF4. {ECO:0000269|PubMed:21205830}. |
| Domain TOPO_DOM 2 |
| Target Relevance information above includes information from UniProt accession : P27824 |
| The UniProt Consortium |
Data
| No results found |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC |
Documents
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