| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | CA-125/MUC16 |
| available sizes | 100 µg |
mouse anti-CA-125 monoclonal antibody (325) 4754
$295.00
Antibody summary
- Mouse monoclonal to CA-125
- Suitable for: WB,ELISA,IHC
- Isotype: IgG1
- 100 µg
mouse anti-CA-125 monoclonal antibody (325) 4754
| antibody |
|---|
| Tested applications WB,IHC,IHC,ELISA |
| Recommended dilutions These antibodies may be used in ELISA and Western blotting to detect CA-125. Positive control: OVCAR3 ovarian adenocarcinoma cells.Suggested pairs for sandwich ELISA: |
| Immunogen CA-125 purified from ovarian carcinoma cells. |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions These antibodies are stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles. |
| Storage buffer 10mM Tris buffer, pH 7.5, 0.15M NaCL, 0.05% NaN |
| Purity ion exchange |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Protein names Mucin-16 (MUC-16) (Ovarian cancer-related tumor marker CA125) (CA-125) (Ovarian carcinoma antigen CA125) |
| Gene names MUC16,MUC16 CA125 |
| Mass 1519175Da |
| Function Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. |
| Subellular location Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. Note=May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain. |
| Tissues Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas. |
| Structure Binds to MSLN. Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN. |
| Post-translational modification Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.; Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.; May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.; May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts. |
| Domain TOPO_DOM 1 |
| Target Relevance information above includes information from UniProt accession: Q8WXI7 |
| The UniProt Consortium |
Data
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| Immunohistochemistry on paraffin-embedded samples, left to right, lung adenocarcinoma, serosal ovarian adenocarcinoma, bladder adenocarcinoma. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
|---|
| Western blot IHC |
Documents
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| Please enter your product and batch number here to retrieve - product datasheet, SDS, and QC information. | |||
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