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mouse anti-Bovine Osteocalcin monoclonal antibody (OCG4) 5454

$509.00

Antibody summary

  • Mouse monoclonal to Bovine Osteocalcin
  • Suitable for: WB,ELISA,IHC
  • Isotype: IgG1
  • 100 µg
SKU: 5454parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgG1

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Osteocalcin

available sizes

100 µg

Available product – mouse anti-Bovine Osteocalcin monoclonal antibody (OCG4) 5454

antibody
Tested applications
WB,IHC,IHC,ELISA
Recommended dilutions
Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50116.

Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions.

Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval.

Endusers should
Immunogen
Bovine osteocalcin
Size and concentration
100µg and
Form
lyophilized
Storage Instructions
Store stock solution (2. 0 mg/ml) in aliquots at -20 for 1 year, or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stor
Storage buffer
PBS, pH7.4, containing 1.0% bovine serum albumin
Purity
immunogen affinty purifcation
Clonality
monoclonal
Isotype
IgG1
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgG1 - Isotype Control
target relevance
Protein names
Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein)
Gene names
BGLAP,BGLAP
Protein family
Osteocalcin/matrix Gla protein family
Mass
10963Da
Function
FUNCTION: Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation (PubMed:3019668, PubMed:6967872). Functions to limit bone formation without impairing bone resorption or mineralization (By similarity). It binds strongly to apatite and calcium (PubMed:6967872). {ECO:0000250|UniProtKB:P86546, ECO:0000269|PubMed:3019668, ECO:0000269|PubMed:6967872}.; FUNCTION: The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development. {ECO:0000250|UniProtKB:P86546}.
Subellular location
SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:6967872}.
Post-translational modification
PTM: Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation by GGCX. These residues are essential for the binding of calcium (By similarity) (PubMed:6967872). Decarboxylation promotes the hormone activity (By similarity). {ECO:0000250|UniProtKB:P86546, ECO:0000255|PROSITE-ProRule:PRU00463, ECO:0000269|PubMed:6967872}.
Target Relevance information above includes information from UniProt accession : P02818
The UniProt Consortium

Data

benchmark-antibodies_anti-osteocalcin_antibody_5454_1.jpg
Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval. Chondroma: Human bone tumor. Synovial Sarcoma: human osteosarcoma.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC

Documents

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