| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG2a |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Osteocalcin |
| available sizes | 100 µg |
mouse anti-Bovine Osteocalcin monoclonal antibody (OC4-30) 1625
$509.00
Antibody summary
- Mouse monoclonal to Bovine Osteocalcin
- Suitable for: WB,ELISA,IHC
- Isotype: IgG2a
- 100 µg
mouse anti-Bovine Osteocalcin monoclonal antibody (OC4-30) 1625
| antibody |
|---|
| Tested applications WB,IHC,IHC,ELISA |
| Recommended dilutions Sandwich ELISA: 1-10ug/ml with immobilized antigen, pair with catalog no. 50120. Immunoblotting: 1-10ug/ml, reducing or non-reducing conditions. Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval. Endusers should |
| Immunogen Bovine osteocalcin |
| Size and concentration 100µg and lot specific |
| Form liquid |
| Storage Instructions Store stock solution (2. 0 mg/ml) in aliquots at -20 for 1 year, or at 4°C for 6 months after adding 0.1% sodium azide. Avoid repeated freeze-thaw cycles. Diluted antibody should not be stor |
| Storage buffer PBS, pH7.4, containing 1.0% bovine serum albumi |
| Purity immunogen affinty purifcation |
| Clonality monoclonal |
| Isotype IgG2a |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG2a - Isotype Control |
| target relevance |
|---|
| Protein names Osteocalcin (Bone Gla protein) (BGP) (Gamma-carboxyglutamic acid-containing protein) |
| Gene names BGLAP,BGLAP |
| Protein family Osteocalcin/matrix Gla protein family |
| Mass 10963Da |
| Function FUNCTION: Bone protein that constitutes 1-2% of the total bone protein, and which acts as a negative regulator of bone formation (PubMed:3019668, PubMed:6967872). Functions to limit bone formation without impairing bone resorption or mineralization (By similarity). It binds strongly to apatite and calcium (PubMed:6967872). {ECO:0000250|UniProtKB:P86546, ECO:0000269|PubMed:3019668, ECO:0000269|PubMed:6967872}.; FUNCTION: The uncarboxylated form acts as a hormone secreted by osteoblasts, which regulates different cellular processes, such as energy metabolism, male fertility and brain development. Regulates of energy metabolism by acting as a hormone favoring pancreatic beta-cell proliferation, insulin secretion and sensitivity and energy expenditure. Uncarboxylated osteocalcin hormone also promotes testosterone production in the testes: acts as a ligand for G protein-coupled receptor GPRC6A at the surface of Leydig cells, initiating a signaling response that promotes the expression of enzymes required for testosterone synthesis in a CREB-dependent manner. Also acts as a regulator of brain development: osteocalcin hormone crosses the blood-brain barrier and acts as a ligand for GPR158 on neurons, initiating a signaling response that prevents neuronal apoptosis in the hippocampus, favors the synthesis of all monoamine neurotransmitters and inhibits that of gamma-aminobutyric acid (GABA). Osteocalcin also crosses the placenta during pregnancy and maternal osteocalcin is required for fetal brain development. {ECO:0000250|UniProtKB:P86546}. |
| Subellular location SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:6967872}. |
| Post-translational modification PTM: Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation by GGCX. These residues are essential for the binding of calcium (By similarity) (PubMed:6967872). Decarboxylation promotes the hormone activity (By similarity). {ECO:0000250|UniProtKB:P86546, ECO:0000255|PROSITE-ProRule:PRU00463, ECO:0000269|PubMed:6967872}. |
| Target Relevance information above includes information from UniProt accession : P02818 |
| The UniProt Consortium |
Data
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| Immunohistochemistry: 1-10ug/ml, frozen or paraffin-embedded tissue after proteinase K (0.4m/ml) antigen retrieval. Chondroma: Human bone tumor. Synovial Sarcoma: human osteosarcoma. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.| pmid | title | authors | citation |
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Protocols
| relevant to this product |
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| Western blot IHC |
Documents
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