| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgG1 |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Bacterial Transglutaminase |
| available sizes | 100 µg |
mouse anti-Bacterial Transglutaminase monoclonal antibody (3C7) 3023
$520.00
Antibody summary
- Mouse monoclonal to Bacterial Transglutaminase
- Suitable for: WB,ELISA
- Isotype: IgG1
- 100 µg
mouse anti-Bacterial Transglutaminase monoclonal antibody (3C7) 3023
| antibody |
|---|
| Tested applications WB,ELISA |
| Recommended dilutions Immunoblotting: use at 1-10ug/mL. A band of ~38kDa is detected ELISA: use at 1-5ug/mL with Streptomyces Tgase on the solid phase. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. |
| Immunogen Recombinant Streptomyces mobaraensis transglutaminase. |
| Size and concentration 100µg and |
| Form lyophilized |
| Storage Instructions This product is stable for at least one (1) year at -20°C to -70°C. Reconstituted product should be stored in appropriate aliquots to avoid repeated freeze-thaw cycles. |
| Storage buffer Lyophilized, 0.1M Tris, 0.1M glycine, 2% sucros |
| Purity protein affinity purification |
| Clonality monoclonal |
| Isotype IgG1 |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgG1 - Isotype Control |
| target relevance |
|---|
| Streptomyces mobaraensis TGAS Protein-glutamine gamma-glutamyltransferase |
| Protein names Protein-glutamine gamma-glutamyltransferase |
| Alternative names MTG, Transglutaminase |
| Protein family Belongs to the bacterial TGase family |
| Function Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins |
| Catalytic activity L-glutaminyl-[protein] + L-lysyl-[protein] = [protein]-L-lysyl-N(6)-5-L-glutamyl-[protein] + NH4(+) |
| Keywords 3D-structure, Acyltransferase, Direct protein sequencing, Signal, Transferase, Zymogen |
| Sequence MRIRRRALVFATMSAVLCTAGFMPSAGEAAADNGAGEETKSYAETYRLTADDVANINALN ESAPAASSAGPSFRAPDSDDRVTPPAEPLDRMPDPYRPSYGRAETVVNNYIRKWQQVYSH RDGRKQQMTEEQREWLSYGCVGVTWVNSGQYPTNRLAFASFDEDRFKNELKNGRPRSGET RAEFEGRVAKESFDEEKGFQRAREVASVMNRALENAHDESAYLDNLKKELANGNDALRNE DARSPFYSALRNTPSFKERNGGNHDPSRMKAVIYSKHFWSGQDRSSSADKRKYGDPDAFR PAPGTGLVDMSRDRNIPRSPTSPGEGFVNFDYGWFGAQTEADADKTVWTHGNHYHAPNGS LGAMHVYESKFRNWSEGYSDFDRGAYVITFIPKSWNTAPDKVKQGWP |
| UniProt accession: P81453 |
Data
 |
| Immunoblotting: use at 1-10ug/ml. A band of ~38kDa is detected. |
FAQ & Publications
Frequently Asked Questions
What is the recommended dilution range for using this mouse anti-Bacterial Transglutaminase monoclonal antibody in Western blotting?
For Western blot applications, it is recommended to use the antibody at a concentration of 1 to 10 µg/mL. At this range, a band of approximately 38 kDa corresponding to bacterial transglutaminase is detected.
How should the mouse anti-Bacterial Transglutaminase monoclonal antibody (3C7) be stored to maintain its stability?
The antibody is stable for at least one year when stored lyophilized at temperatures between -20°C and -70°C. After reconstitution, it should be aliquoted appropriately to avoid repeated freeze-thaw cycles, which can reduce stability.
What is the host species and isotype of the monoclonal antibody against bacterial transglutaminase?
This monoclonal antibody is produced in mouse and is of the IgG1 isotype.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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