Description Determined by a competitive inhibition ELISA with nucleoside variants. The %B/B0 is plotted as a function of the concentration (nM) of the indicated competitor. 100% B/B0 indicates no inhibition by the indicated competitor.

Description Selectivity and Specificity of 6E4 in DNA: Binds 8-oxoA in single and double stranded DNA Recognizes 8-oxoA in all DNA base mispairs

Description Selectivity and Specificity of 6E4 in RNA: Binds 8-oxoA in single and double stranded RNA Recognizes 8-oxoA in RNA base mispairs with standard geometry

mouse anti-8-oxo Adenosine DNA/RNA damage monoclonal antibody (6E4) 1002

$220.00 – $450.00

Antibody summary

Mouse monoclonal to 8-oxo-adenosine
Suitable for: ELISA
Reacts with: All
Isotype: IgG1
100 µg, 500 µg

available sizes

Clear

SKU: 1002parent Categories: antibody, oxidative stress Tag: DNA/RNA damage

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	1 mg/mL
applications	ELISA
reactivity	all, human
available sizes	100 µg, 500 µg

mouse anti-8-oxo Adenosine DNA/RNA damage monoclonal antibody (6E4) 1002

antibody

Tested applications ELISA
Recommended dilutions Dilute in PBS or medium that is identical to that used in the assay system. Optimal concentrations must be derived for specific applications.
Immunogen 8-oxo-adenosine
Size and concentration 100, 500µg and 1 mg/mL
Form liquid
Storage Instructions Store antibody at -20C to -80C. Avoid multiple freeze-thaw cycles.
Storage buffer PBS, pH 7.4
Purity protein G affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names 8-oxoadenosine, 8-oxoA, 8-OH-A, 8-hydroxyadenosine

Data

Determined by a competitive inhibition ELISA with nucleoside variants. The %B/B0 is plotted as a function of the concentration (nM) of the indicated competitor. 100% B/B0 indicates no inhibition by the indicated competitor

Selectivity and Specificity of 6E4 in RNA: Binds 8-oxoA in single and double stranded RNA Recognizes 8-oxoA in RNA base mis-pairs with standard geometry

Selectivity and Specificity of 6E4 in DNA: Binds 8-oxoA in single and double stranded DNA Recognizes 8-oxoA in all DNA base mispairs

Publications

pmid	title	authors	citation
36989571	Discovery and properties of a monoclonal antibody targeting 8-oxoA, an oxidized adenine lesion in DNA and RNA.	Daniel J Tew, Jakob M Hebert, Brad J Schmier	Redox Biol 62:102658

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.