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Human Bordetalla Pertussis IgA Lateral flow dipstick kit 5773

Human Bordetalla Pertussis IgA Lateral flow dipstick kit 5773

$487.00

Summary

  • Mikrogen diagnostik lateral flow device (dipstick) for research use (RUO)
  • Human Bordetalla Pertussis IgA Lateral flow dipstick kit 5773
  • Suitable for IgA detection
  • Ready-to-use
  • 20 tests
SKU: 5773 Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
target

Human Bordetalla Pertussis IgA

species reactivity

Bordetella pertussis, Bordetella parapertussis

applications

Lateral flow (dipstick)

assay type

Indirect & qualitative

available sizes

2x 20 test kits

Human Bordetalla Pertussis IgA Lateral flow dipstick kit 5773

kit
Assay type
Sandwich assay, lateral flow (dipstick)
Research area
Infectious Disease
Sample type
Serum, plasma, whole blood
Components
10X Wash Buffer100 mL
TMB Substrate40 mL
Milk Powder5 g
Instructions for Use1 Each
Evaluation Form1 Each
Test Strips2 kits of [2 Vials x 10 Each]
Anti-Human IgA Conjugate500 μL
Positive Control140 μL
Negative Control140 μL
Storage
Store at 2-8°C.
Additional information

The Mikrogen recomLine Bordetella pertussis tests contain the two antigens pertussis toxin (PT or PT-100), specific for B. pertussis, and filamentous hemagglutinin (FHA), which all representatives of the Bordetella species possess.

After screening by ELISA, the recomLine Bordetella pertussis IgG, IgA can be used to clarify inconclusive or positive ELISA results to determine infection status.

Advantages
  • PT-100 antigen concentration calibrated by the WHO standard.
  • IgG antibody concentrations of ?100 IU/ml against pertussis toxin - indicating acute pertussis infection.
  • Antibodies against filamentous hemagglutinin antigen (FHA) indicate Bordetella species infection.
  • Separate detection of IgG and IgA antibodies.
  • Simple and clear interpretation due to easy-to-read banding.
  • Partial and full automation, software-based evaluation (recomScan), and integration with laboratory information system possible.
  • Highest sensitivity and specificity through use of recombinant antigens:
Bands
AntigenAbbreviationMolecular Weight [kDa]
Filamentous hemagglutininFHA35
Pertussis toxin*PT-100, PT28, 23, 22, 11.7, 9.3 (5 subunits)
target relevance
Organism
Bordetella pertussis
Structure and strains
Bordetella pertussis is a Gram-negative, aerobic, pathogenic, encapsulated coccobacillus of the genus Bordetella, and the causative agent of pertussis or whooping cough. Like B. bronchiseptica, B. pertussis can express a flagellum-like structure, even if it has been historically categorized as a nonmotile bacteria.[1] Its virulence factors include pertussis toxin, adenylate cyclase toxin, filamentous hemagglutinin, pertactin, fimbria, and tracheal cytotoxin.
Disease
Bordetella pertussis and Bordetella parapertussis are pathogens responsible for whooping cough, a worldwide spread infectious disease that is transmitted from person to person by droplet infection. Often young children up to four years old are affected. The mortality in infected infants is particularly high. Although young people and adults usually do not get seriously ill, they may act as a source of infection for non-protected and at risk patients such as infants and old people. Colonization of the respiratory tract and establishment of infection are facilitated by the synergistic action of several virulence factors.

Progression of a typical whooping cough can be divided into three stages. After an incubation period of one to two weeks symptoms begin with the catarrhal phase, usually accompanied by a cough, rhinitis and conjunctivitis. Subsequently, the convulsive phase follows characterized by paroxysmal cough attacks combined with vomiting of viscid mucus, laryngospasm and bronchospasm, leading to cyanosis in the child. After four to six weeks attacks diminish and slowly subside in the decrementi phase.
Detection and diagnosis
The ELISA technique is the most commonly chosen method for B. pertussis and B. parapertussis specific antibody determination in serodiagnosis and complements direct antigen detection. In over 90% of cases the IgG and IgA antibody responses are directed against the immunogens PT and FHA. Therefore, the SERION ELISA classic Bordetella pertussis IgG and IgA tests are based on antigen mixtures of PT and FHA. For the detection of IgM antibodies cellular bound lipopolysaccharid has outstanding diagnostic properties. Consequently, a whole-cell antigen preparation of B. pertussis is used in the SERION ELISA classic Bordetella pertussis IgM.

Publications

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Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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