Human Parvovirus B19 IgM Lateral flow dipstick kit 4473
kit
Assay type Sandwich assay, lateral flow (dipstick)
Research area Infectious Disease
Sample type Serum, plasma, whole blood
Components
10X Wash Buffer
100 mL
TMB Substrate
40 mL
Milk Powder
5 g
Instructions for Use
1 Each
Evaluation Form
1 Each
Test Strips
2 kits of [2 Vials x 10 Each]
Anti-Human IgM Conjugate
500 µL
Positive Control
140 µL
Negative Control
140 µL
Storage Store at 2-8°C.
Additional information Mikrogen recomLine Parvovirus B19 Test
The Mikrogen recomLine Parvovirus B19 tests allow an indication of the time of infection due to the reactivity patterns of the recombinant antigens and the possibility to detect avidity determination of the IgG antibodies.
The determination of antibodies against the NS-1 antigen can be helpful in clarifying persistent parvovirus B19 infections. By using VP-2 particles, a presentation of conformational epitopes to the otherwise presented linear epitopes is achieved.
Advantages
Separate detection of IgG and IgM antibodies with antibody class control on the test strip.
Band pattern and avidity determination allow conclusions on infection status.
Parvovirus B19 infections during the last 4 weeks can be reliably excluded.
Excellent diagnostic quality due to internal controls and the use of VP2 particles.
Easy and clear interpretation, featuring easy-to-read banding patterns.
Partial and full automation, software-based evaluation (recomScan), and integration with the laboratory information system are possible.
Highest sensitivity and specificity due to the use of recombinant antigens.
Bands
Antigen
Abbreviation
Size (kDa)
Main capsid antigen (conformational epitopes)
VP-2p
65
N-terminal half of the structural proteins VP-1 and VP-2
VP-N
60
VP-1 specific segment (differentiation from VP-2)
VP-1S
31
Main capsid antigen (linear epitopes)
VP-2r
56
C-terminal half of the structural proteins VP-1 and VP-2
VP-C
42
Non-structural protein NS-1
NS-1
75
target relevance
human antibody anti-Parvovirus
Parvovirus
Description Group of viruses that cause gastrointestinal, respiratory, and systemic infections in humans and animals
Structure Parvoviruses are small, non-enveloped viruses containing a linear single-stranded DNA genome and belonging to the family Parvoviridae. The viral capsid possesses an icosahedral structure and lacks a lipid envelope, contributing to exceptional environmental stability and resistance to many common disinfectants. Members of the Parvoviridae family infect a wide range of vertebrate and invertebrate hosts and include important human and veterinary pathogens. Human Parvovirus B19 is the best-known human pathogen of the group and is the causative agent of erythema infectiosum (fifth disease). Other parvoviruses, such as canine parvovirus and feline panleukopenia virus, cause severe disease in animals. Parvoviruses preferentially infect rapidly dividing cells because they rely on host cellular machinery for viral DNA replication.
Diagnosis The diagnosis of parvovirus infection depends on the specific virus involved and may include serological, molecular, and antigen detection methods. Human Parvovirus B19 infection is commonly diagnosed through the detection of virus-specific IgM and IgG antibodies or by polymerase chain reaction (PCR) assays that identify viral DNA in blood or tissue samples. In veterinary medicine, infections such as canine parvovirus are frequently diagnosed using ELISA-based antigen detection tests on fecal samples, while PCR provides highly sensitive confirmation and strain identification. Laboratory findings and clinical presentation are often used in conjunction with specific diagnostic tests to establish the diagnosis.
Symptoms Parvovirus infections produce a broad spectrum of clinical manifestations depending on the host species and the specific virus involved. Human Parvovirus B19 infection commonly causes erythema infectiosum, characterized by a distinctive 'slapped-cheek' rash, mild fever, headache, and malaise. In adults, joint pain and arthritis-like symptoms may occur. In immunocompromised individuals or patients with underlying hematological disorders, severe anemia may develop due to infection of erythroid precursor cells. During pregnancy, fetal infection may result in hydrops fetalis or fetal loss. Animal parvoviruses, particularly canine parvovirus, commonly cause severe gastrointestinal disease characterized by vomiting, hemorrhagic diarrhea, dehydration, lethargy, and potentially fatal secondary infections.
Data
FAQ & Publications
Frequently Asked Questions
What sample types are compatible with the Human Parvovirus B19 IgM Lateral Flow Dipstick Kit 4473?
The kit is compatible with serum, plasma, and whole blood samples for the detection of Human Parvovirus B19 IgM antibodies.
How should the Human Parvovirus B19 IgM Lateral Flow Dipstick Kit 4473 be stored to maintain its stability?
The kit components should be stored at 2-8°C to ensure proper stability and performance.
Publications
pmid
title
authors
citation
We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU). 4473 protocol
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