Human Helicobacter IgA Lateral flow dipstick kit 4775
kit
Assay type Sandwich assay, lateral flow (dipstick)
Research area Infectious Disease
Sample type Serum, plasma, whole blood
Components
10X Wash Buffer
100 mL
TMB Substrate
40 mL
Milk Powder
5 g
Instructions for Use
1 Each
Evaluation Form
1 Each
Test Strips
2 kits of [2 Vials x 10 Each]
Anti-Human IgA Conjugate
500 µL
Positive Control
140 µL
Negative Control
140 µL
Storage Store at 2-8°C.
Additional information
Mikrogen recomLine Helicobacter 2.0 tests are serological, qualitative in vitro line immunoassays for the detection of antibodies
against ten selected Helicobacter pylori antigens. The detection of antibodies against the two most important virulence
factors CagA and VacA are of high diagnostic importance. These two virulence factors significantly increase the risk for premalignant
changes, gastric carcinoma, MALT lymphoma, and ulcers. Humoral immune responses against the GroEL, HtrA, NapA, HP231, and CtkA
biomarkers are associated with more severe disease progression.
Advantages
Sensitivity: > 99% IgG positive results of gold standard positive patients.
Specificity: 100% IgG and IgA negative results of gold standard negative patients.
Excellent correlation with the gold standard of Helicobacter pylori diagnostics - culture and histology.
Reliable results at all times, even with low colonization density.
Identification of highly virulent Helicobacter pylori type I infections using CagA.
Detection of antibodies against ten relevant biomarkers and virulence factors associated with more severe disease progression.
Simple and clear interpretation through easy-to-read banding.
Partial and full automation, software-based evaluation (recomScan), and integration with laboratory information system possible.
Highest sensitivity and specificity through the use of recombinant antigens:
target relevance
human antibody anti-Helicobacter pylori
Helicobacter pylori
Description Bacterium that causes gastritis, peptic ulcer disease, and gastric cancer
Structure Helicobacter pylori is a Gram-negative, microaerophilic, spiral-shaped bacterium belonging to the family Helicobacteraceae. The organism is uniquely adapted to colonize the human stomach and is capable of surviving the highly acidic gastric environment through the production of urease, an enzyme that converts urea into ammonia and carbon dioxide, creating a protective alkaline microenvironment. H. pylori possesses multiple flagella that enable motility through the gastric mucus layer and adherence to gastric epithelial cells. The bacterium is one of the most common chronic bacterial infections worldwide and is recognized as a major cause of chronic gastritis, peptic ulcer disease, mucosa-associated lymphoid tissue (MALT) lymphoma, and gastric adenocarcinoma.
Diagnosis The diagnosis of Helicobacter pylori infection can be performed using invasive and non-invasive methods. Non-invasive tests include urea breath tests, stool antigen assays, and serological detection of H. pylori-specific antibodies. The urea breath test and stool antigen assays are highly sensitive and specific and are commonly used for both diagnosis and confirmation of eradication following treatment. Invasive diagnostic methods involve upper gastrointestinal endoscopy with gastric biopsy for histological examination, rapid urease testing, culture, or molecular detection by polymerase chain reaction (PCR). Serological assays, including ELISA-based methods, are useful for epidemiological studies and assessment of previous exposure but may not reliably distinguish active from past infection.
Symptoms Helicobacter pylori infection is often acquired during childhood and may persist for life if untreated. Many infected individuals remain asymptomatic; however, chronic infection can lead to inflammation of the gastric mucosa and the development of gastrointestinal disease. Common symptoms include upper abdominal pain or discomfort, nausea, bloating, early satiety, excessive belching, and indigestion. In patients with peptic ulcer disease, symptoms may include burning epigastric pain, gastrointestinal bleeding, vomiting, or anemia. Long-term infection increases the risk of gastric atrophy, intestinal metaplasia, gastric adenocarcinoma, and MALT lymphoma. Eradication of H. pylori significantly reduces the risk of ulcer recurrence and certain gastric malignancies.
Data
FAQ & Publications
Frequently Asked Questions
What sample types are compatible with the Human Helicobacter IgA Lateral Flow Dipstick Kit 4775?
The kit is compatible with serum, plasma, and whole blood samples for the detection of Human Helicobacter IgA antibodies.
How should the Human Helicobacter IgA Lateral Flow Dipstick Kit 4775 be stored to maintain stability?
The kit should be stored at 2-8°C to ensure optimal stability and performance.
Publications
pmid
title
authors
citation
We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
relevant to this product
This product has moved to a digital protocol. Please use the URL provided on the product packaging to access the electronic Instructions for Use (eIFU). 4775 protocol
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