ICC/IF image goat anti NeuN polyclonal antibody 7454

goat anti-NeuN polyclonal antibody 7454

$100.00 – $2,600.00

Antibody summary

Goat polyclonal to NeuN
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

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SKU: 7454parent Categories: antibody, cell marker Tag: NeuN

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	goat
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

goat anti-NeuN polyclonal antibody 7454

antibody
Database link: human A6NFN3 mouse Q8BIF2
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions Western blot: 1:1000-1:2000 IF/IHC 1:1000-1:5000
Immunogen N-terminal 100 amino acids of human Fox3 expressed in and purified from E. coli
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control Goat polyclonal - Isotype Control

target relevance
NeuN, also known as Fox-3 or RBFOX3, is a well-established neuronal marker widely used in neuroscience research. It is a nuclear protein that is selectively expressed in post-mitotic neurons, making it an essential tool for identifying and characterizing neuronal populations in various tissues and experimental models. NeuN immunohistochemistry, facilitated by specific antibodies targeting NeuN, allows researchers to visualize and quantify neuronal cell bodies, providing valuable insights into neuronal distribution, density, and morphology. This marker is particularly valuable for studying neurodevelopment, neurodegenerative diseases, and neuronal plasticity in response to different experimental conditions or treatments. Moreover, NeuN antibodies have proven instrumental in identifying and differentiating neurons from other cell types, such as glial cells, in complex neural tissues. Click for more on: cell markers and NeuN
Protein names RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen)
Gene names RBFOX3,RBFOX3
Mass 33873Da
Function FUNCTION: Pre-mRNA alternative splicing regulator. Regulates alternative splicing of RBFOX2 to enhance the production of mRNA species that are targeted for nonsense-mediated decay (NMD). {ECO:0000250\|UniProtKB:Q8BIF2}.
Subellular location SUBCELLULAR LOCATION: Nucleus {ECO:0000269\|PubMed:24215932}. Cytoplasm {ECO:0000269\|PubMed:24215932}. Note=Largely restricted to neuronal nuclei. However, significant cytoplasmic localization in neurons from brains from HIV-infected individuals with cognitive impairment. {ECO:0000269\|PubMed:24215932}.
Target Relevance information above includes information from UniProt accession: A6NFN3
The UniProt Consortium

Data

Immunofluorescent analysis of a section of adult rat hippocampus stained with goat pAb to Fox3/NeuN, 7454, dilution 1:2,000 in red, costained with mouse monoclonal antibdy to MAP2 dilution 1:2,000, in green. Nuclear DNA was revealed in blue using the DAPI stain. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The Fox3/NeuN antibody stains the nuclei of neurons in the hippocampus while the MAP2 antibody stains the dendritic processes of neurons.

Chromogenic immunostaining of a formalin fixed paraffin embedded rat hippocampus section with goat pAb to FOX3/NeuN, 7454, dilution 1:2,000, detected with DAB (brown) using the Vector Elite 8718C-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The FOX3/NeuN antibody labels the nuclei of neurons in the hippocampus.

Western blot analysis of whole brain lysates using goat pAb to FOX3/NeuN, 7454, dilution 1:1,000 in green: [1] protein standard (red), [2] mouse brain, [3] rat brain. Bands at 46k and 48 kDa correspond to protein isotypes of FOX3/NeuN.

Publications

pmid	title	authors	citation
19713214	Identification of neuronal nuclei (NeuN) as Fox-3, a new member of the Fox-1 gene family of splicing factors.	Kee K Kim, Robert S Adelstein, Sachiyo Kawamoto	J Biol Chem 284:31052-61
16260614	Homologues of the Caenorhabditis elegans Fox-1 protein are neuronal splicing regulators in mammals.	Jason G Underwood, Paul L Boutz, Joseph D Dougherty, Peter Stoilov, Douglas L Black	Mol Cell Biol 25:10005-16
7821230	Identification of a candidate primary sex determination locus, fox-1, on the X chromosome of Caenorhabditis elegans.	J Hodgkin, J D Zellan, D G Albertson	Development 120:3681-9

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.