goat anti-Ki67 polyclonal antibody 1343

$100.00 – $2,600.00

Antibody summary

Goat polyclonal to Ki67
Suitable for: WB, ICC/IF
Reacts with: human
Isotype: IgG
100 µg, 25 µg, 1 mg

SKU: 1343parent Categories: antibody, cell marker Tag: Ki-67

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	goat
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	human
available sizes	1 mg, 100 µg, 25 µg

goat anti-Ki67 polyclonal antibody 1343

antibody
Database link: human P46013
Tested applications WB,ICC/IF
Recommended dilutions WB: 1:10000 IF: 1:1000-5000
Immunogen Internal region, amino acids 1111-1490 of human recombinant Ki-67 protein
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control Goat polyclonal - Isotype Control

target relevance
ki-67 is a widely used cell marker in research, particularly in the field of cell proliferation and cancer biology. It is a nuclear protein that is present during active phases of the cell cycle (G1, S, G2, and M phases) but absent in resting cells (G0 phase). As a marker of cellular proliferation, ki-67 is utilized to assess the growth fraction of a cell population and to quantify the proportion of actively dividing cells within a tissue or sample. Immunohistochemistry and immunofluorescence techniques employing antibodies targeting ki-67 are commonly employed to visualize and quantify its expression in tissues and cell cultures. Click for more on: cell markers and ki-67
Protein names Proliferation marker protein Ki-67 (Antigen identified by monoclonal antibody Ki-67) (Antigen KI-67) (Antigen Ki67)
Gene names MKI67,MKI67
Mass 358694Da
Function Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226). Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226). Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226). Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551). Does not contribute to the internal structure of mitotic chromosomes (By similarity). May play a role in chromatin organization (PubMed:24867636). It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).
Subellular location Chromosome Nucleus Nucleus, nucleolus Note=Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the mitotic chromosome surface (PubMed:27362226). Associates with satellite DNA in G1 phase (PubMed:9510506). Binds tightly to chromatin in interphase, chromatin-binding decreases in mitosis when it associates with the surface of the condensed chromosomes (PubMed:15896774, PubMed:22002106). Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix (PubMed:22002106).
Structure Interacts with KIF15 (PubMed:10878014). Interacts (via the FHA domain) with NIFK (PubMed:11342549, PubMed:14659764, PubMed:16244663). Interacts with PPP1CC (PubMed:24867636). Component of a complex at least composed of ZNF335, HCFC1, CCAR2, EMSY, MKI67, RBBP5, ASH2L and WDR5; the complex is formed as a result of interactions between components of a nuclear receptor-mediated transcription complex and a histone methylation complex (PubMed:19131338). Interacts with ZNF335 (PubMed:19131338, PubMed:23178126).
Post-translational modification Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604). Hyperphosphorylated form does not bind DNA.
Biotechnology Widely used as a marker to assess cell proliferation, as it is detected in the nucleus of proliferating cells only (PubMed:6339421, PubMed:21960707). In cancer research field for example, MKI67 is the most widely used marker for comparing proliferation between tumor samples (PubMed:21960707, PubMed:26680267).
Target Relevance information above includes information from UniProt accession: P46013
The UniProt Consortium

Data

Immunofluorescent analysis of HeLa cell culture stained with goat pAb to vimentin, 1343, dilution 1:500 in green, and costained with mouse mAb to actin, 7047, dilution 1:500 in red. Blue is Hoechst staining of nuclear DNA. 1343 antibody produces strong staining of intermediate filament network of the cytoskeleton, while actin antibody stains the submembranous actin-rich cytoskeleton, stress fibers and bundles of actin associated with cell adhesion sites.

Western blot analysis of different cell lysates using goat pAb to vimentin, 1343, dilution 1:5,000 in green. [1] protein standard, [2] HeLa, [3] HEK293, [4] NIH-3T3 and [5] C6 cell lysates. The 1343 antibody binds to the vimentin protein showing a single band at ~50kDa.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.