| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | chicken |
| isotype | IgY |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | human, mouse, rat |
| available sizes | 1 mg, 100 µg, 25 µg |
chicken anti-Vimentin polyclonal antibody 6886
$100.00 – $2,600.00
Antibody summary
- Chicken polyclonal to Vimentin
- Suitable for: WB, ICC/IF
- Reacts with: human, mouse, rat
- Isotype: IgY
- 100 µg, 25 µg, 1 mg
chicken anti-Vimentin polyclonal antibody 6886
| target relevance |
|---|
| Vimentin, a key protein constituent of the intermediate filament cytoskeleton, plays a pivotal role in maintaining cellular integrity and structure. In research, vimentin has become a valuable biomarker due to its tissue-specific expression and involvement in various cellular processes. Immunohistochemical analysis of vimentin has been extensively utilized to identify and characterize mesenchymal cells, such as fibroblasts, endothelial cells, and immune cells, aiding in the study of tissue development, wound healing, and cancer progression. Additionally, vimentin has been linked to epithelial-mesenchymal transition (EMT), a critical process implicated in embryonic development and metastasis. Furthermore, efforts to develop vimentin-targeting therapies for cancer treatment have shown promise, as vimentin's involvement in EMT and tumor progression make it a potential therapeutic target for limiting cancer metastasis. Click for more on: cell markers and Vimentin |
| Protein names Vimentin |
| Gene names VIM,VIM |
| Protein family Intermediate filament family |
| Mass 53652Da |
| Function Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. ; Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2. |
| Subellular location Cytoplasm Cytoplasm, cytoskeleton Nucleus matrix Cell membrane |
| Tissues Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines. |
| Structure Homomer assembled from elementary dimers (PubMed:20176112). Identified in complexes that contain VIM, EZR, AHNAK, BFSP1, BFSP2, ANK2, PLEC, PRX and spectrin (By similarity). Interacts with BCAS3 (PubMed:17505058). Interacts with LGSN (By similarity). Interacts with SYNM (By similarity). Interacts (via rod region) with PLEC (via CH 1 domain) (By similarity). Interacts with PLEC isoform 1C (PubMed:24940650). Interacts with STK33 (PubMed:18811945). Interacts with LARP6 (PubMed:21746880). Interacts with RAB8B (By similarity). Interacts with TOR1A; the interaction associates TOR1A with the cytoskeleton (PubMed:16361107, PubMed:18827015). Interacts with TOR1AIP1 (PubMed:16361107). Interacts with DIAPH1 (PubMed:23325789). Interacts with EPPK1; interaction is dependent of higher-order structure of intermediate filament (PubMed:16923132). Interacts with the non-receptor tyrosine kinase SRMS; the interaction leads to phosphorylation of VIM (PubMed:29496907). Interacts with NOD2 (PubMed:27812135). Interacts (via head region) with CORO1C (By similarity). Interacts with HDGF (isoform 2) (PubMed:26845719). Interacts with PRKCE (via phorbol-ester/DAG-type 2 domain) (PubMed:18408015). Interacts with BFSP2 (By similarity). Interacts with PPL (By similarity). Interacts (via rod domain) with PKP1 (PubMed:10852826). Interacts with PKP2 (PubMed:10852826). ; (Microbial infection) Interacts with HCV core protein. |
| Post-translational modification Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin (By similarity). One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm (PubMed:21465480). Phosphorylated by STK33 (PubMed:18811945). Phosphorylated on tyrosine residues by SRMS (PubMed:29496907). ; O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. ; S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex. |
| Involvement in disease Cataract 30, multiple types (CTRCT30) [MIM:116300]: An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. Note=The disease is caused by variants affecting the gene represented in this entry. |
| Target Relevance information above includes information from UniProt accession: P08670 |
| The UniProt Consortium |
Data
 |
| Immunofluorescent analysis of HeLa cell culture stained with chicken pAb to vimentin, 6886, dilution 1:10,000 in green, and costained with EnCor mouse mAb to actin, 7047, dilution 1:500 in red. The blue is DAPI staining of nuclear DNA. The vimentin antibody stains the intermediate filament network while the actin antibody labels the submembranous cytoskeleton, stress fibers, and bundles of actin associated with cell adhesion sites. |
 |
| Neuromics hN2 are human neuronal lineage cells which originate from ArunA Biomedical, and were derived from the human embryonic cell line WA09. We obtained the hN2 Human Neurons Discovery kit from Neuromics, which arrived frozen and on dry ice. We fixed and stained them with our chicken polyclonal antibody to vimentin 6886, in red. Islands of hN2 cells form after 4 days in culture forming beautiful flower like structures. Vimentin is a well established marker of early differentiating neuronal lineage cells, though it is found in many other kinds of cells. Merged from several images taken with a 10X objective lens, this image is also available as a high resolution poster. Blue staining is nuclear DNA. |
 |
| Western blot analysis of tissue and cell lysates using chicken pAb to Vimentin, 6886, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. 6886 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, 5291, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes while the ~70kDa bands are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells. |
Publications
Frequently Asked Questions1. Q: What is the concentration of the Chicken Anti-Vimentin Polyclonal Antibody #6886?
A: The concentration of the antibody is 1 mg/ml, ensuring high quality for your research needs.
2. Q: How should I store the Chicken Anti-Vimentin Polyclonal Antibody #6886?
A: This antibody should be stored at -20°C upon arrival, and can be stored at -20°C for up to one year without losing its activity.
3. Q: Is the Chicken Anti-Vimentin Polyclonal Antibody #6886 suitable for use in immunohistochemistry (IHC)?
A: Yes, this antibody is validated for use in immunohistochemistry (IHC), providing reliable and consistent results in tissue samples.
4. Q: Can I use the Chicken Anti-Vimentin Polyclonal Antibody #6886 in Western blotting applications?
A: Yes, this antibody has been tested and validated for Western blotting, allowing for the detection of Vimentin protein in various samples.
5. Q: Is the Chicken Anti-Vimentin Polyclonal Antibody #6886 compatible with flow cytometry?
A: While this antibody has not been specifically validated for flow cytometry, it may be suitable for use in flow cytometry applications with proper optimization and validation.
A: The concentration of the antibody is 1 mg/ml, ensuring high quality for your research needs.
2. Q: How should I store the Chicken Anti-Vimentin Polyclonal Antibody #6886?
A: This antibody should be stored at -20°C upon arrival, and can be stored at -20°C for up to one year without losing its activity.
3. Q: Is the Chicken Anti-Vimentin Polyclonal Antibody #6886 suitable for use in immunohistochemistry (IHC)?
A: Yes, this antibody is validated for use in immunohistochemistry (IHC), providing reliable and consistent results in tissue samples.
4. Q: Can I use the Chicken Anti-Vimentin Polyclonal Antibody #6886 in Western blotting applications?
A: Yes, this antibody has been tested and validated for Western blotting, allowing for the detection of Vimentin protein in various samples.
5. Q: Is the Chicken Anti-Vimentin Polyclonal Antibody #6886 compatible with flow cytometry?
A: While this antibody has not been specifically validated for flow cytometry, it may be suitable for use in flow cytometry applications with proper optimization and validation.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| 36529126 | Expression of Wilms' Tumor 1 Antigen, Vimentin, and Corticotropin-Releasing Factor in the Human Kidney with Focal Segmental Glomerulosclerosis and Effect of Oxidative Stress on These Markers in HEK 293 Cells | Csurgyók R, Sütő G, Wittmann I, Vas T. | Kidney Blood Press Res. 2023;48(1):56-65. doi: 10.1159/000528727. Epub 2022 Dec 16. |
| 36200046 | Expression of vimentin alters cell mechanics, cell-cell adhesion, and gene expression profiles suggesting the induction of a hybrid EMT in human mammary epithelial cells | Sivagurunathan S, Vahabikashi A, Yang H, Zhang J, Vazquez K, Rajasundaram D, Politanska Y, Abdala-Valencia H, Notbohm J, Guo M, Adam SA, Goldman RD. | Front Cell Dev Biol. 2022 Sep 19;10:929495. doi: 10.3389/fcell.2022.929495. eCollection 2022. |
| 36078136 | FLIM for Evaluation of Difference in Metabolic Status between Native and Differentiated from iPSCs Dermal Papilla Cells | Kashirina A, Gavrina A, Mozherov A, Kozlov D, Kuznetsova D, Vorotelyak E, Zagaynova E, Kalabusheva E, Kashina A. | Cells. 2022 Sep 1;11(17):2730. doi: 10.3390/cells11172730. |
| 35742824 | CX3CL1 Action on Microglia Protects from Diet-Induced Obesity by Restoring POMC Neuronal Excitability and Melanocortin System Activity Impaired by High-Fat Diet Feeding | Banerjee J, Dorfman MD, Fasnacht R, Douglass JD, Wyse-Jackson AC, Barria A, Thaler JP. | Int J Mol Sci. 2022 Jun 7;23(12):6380. doi: 10.3390/ijms23126380. |
| 35606540 | Hypothalamic remodeling of thyroid hormone signaling during hibernation in the arctic ground squirrel | Chmura HE, Duncan C, Saer B, Moore JT, Barnes BM, Loren Buck C, Christian HC, Loudon ASI, Williams CT. | Commun Biol. 2022 May 23;5(1):492. doi: 10.1038/s42003-022-03431-8. |
Protocols
| relevant to this product |
|---|
| Western blot ICC |
Documents
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