| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | chicken |
| isotype | IgY |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, IHC, WB |
| available sizes | 1 mg, 100 µg, 25 µg |
chicken anti-GFAP polyclonal antibody 6048
$100.00 – $2,600.00
Antibody summary
- Chicken polyclonal to GFAP
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgY
- 100 µg, 25 µg, 1 mg
chicken anti-GFAP polyclonal antibody 6048
| target relevance |
|---|
| Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders. Click for more on: cell markers and GFAP |
| Protein names Glial fibrillary acidic protein (GFAP) |
| Gene names GFAP,GFAP |
| Protein family Intermediate filament family |
| Mass 49880Da |
| Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
| Subellular location Cytoplasm Note=Associated with intermediate filaments. |
| Tissues Expressed in cells lacking fibronectin. |
| Structure Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus). |
| Post-translational modification Phosphorylated by PKN1. |
| Target Relevance information above includes information from UniProt accession : P14136 |
| The UniProt Consortium |
Data
 |
| Immunofluorescent analysis of a section of mouse hippocampus stained with chicken pAb to GFAP, 6048, dilution 1:5,000 in green and costained with rabbit pAb to FOX3/NeuN, 2615, dilution 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons. |
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| Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat costained with chicken pAb to Glial Fibrillary Acidic Protein-GFAP, 6048, dilution 1:2,000 in red, and mouse mAb to MAP-τ (tau) 40680, dilution 1:2,000 in green. The blue is DAPI staining of nuclear DNA. The GFAP labels astroglial cells, while the MAP-τ antibody stains neuronal cell perikarya, dendrites and axons. Mouse select image for larger view. |
 |
| Above is an image made by Dr. Fatimeh Shaerzadeh, working in the lab of Professor Habibeh Khoshbouei in the McKnight Brain Institute. A section of mouse frontal cerebral cortex was stained with EnCor chicken polyclonal antibody to GFAP 6048 in green and DNA in blue. The fibrous component of the processes of astroctyes are clearly revealed. Mouse select on image for larger view. |
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| Chromogenic Immunostaining of a formalin fixed paraffin embedded human cerebellum section with chicken pAb to GFAP, 6048, dilution 1:20,000, detected in DAB (brown) following the 8718C method. Hematoxylin (blue) was used as the counterstain. The GFAP antibody detects the core of processes of astrocytes and Bergman glia within the granular and molecular layers. Mouse select image for larger view. |
 |
| Western blot analysis of whole brain lysates using chicken pAb to GFAP, 6048, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain. The strong band at about 50 kDa corresponds to the GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 546 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 36828035 | WWOX P47T partial loss-of-function mutation induces epilepsy, progressive neuroinflammation, and cerebellar degeneration in mice phenocopying human SCAR12 | Hussain T, Sanchez K, Crayton J, Saha D, Jeter C, Lu Y, Abba M, Seo R, Noebels JL, Fonken L, Aldaz CM. | Prog Neurobiol. 2023 Apr;223:102425. doi: 10.1016/j.pneurobio.2023.102425. Epub 2023 Feb 23. |
| 36640336 | Neuronal and astrocytic contributions to Huntington's disease dissected with zinc finger protein transcriptional repressors | Gangwani MR, Soto JS, Jami-Alahmadi Y, Tiwari S, Kawaguchi R, Wohlschlegel JA, Khakh BS. | Cell Rep. 2023 Jan 31;42(1):111953. doi: 10.1016/j.celrep.2022.111953. Epub 2023 Jan 7. |
| 36633130 | ASK1 inhibitor NQDI‑1 decreases oxidative stress and neuroapoptosis via the ASK1/p38 and JNK signaling pathway in early brain injury after subarachnoid hemorrhage in rats | Duan J, Yuan W, Jiang J, Wang J, Yan X, Liu F, Liu A. | Mol Med Rep. 2023 Feb;27(2):47. doi: 10.3892/mmr.2023.12934. Epub 2023 Jan 12. |
| 36614107 | Cerebral Folate Metabolism in Post-Mortem Alzheimer's Disease Tissues: A Small Cohort Study | Naz N, Naqvi SF, Hohn N, Whelan K, Littler P, Roncaroli F, Robinson AC, Miyan JA. | Int J Mol Sci. 2022 Dec 30;24(1):660. doi: 10.3390/ijms24010660. |
| 36593462 | Intracellular deposits of amyloid-beta influence the ability of human iPSC-derived astrocytes to support neuronal function | Konstantinidis E, Portal B, Mothes T, Beretta C, Lindskog M, Erlandsson A. | J Neuroinflammation. 2023 Jan 3;20(1):3. doi: 10.1186/s12974-022-02687-5. |
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
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| No results found | |||
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