ICC/IF image chicken anti GFAP polyclonal antibody 6048

chicken anti-GFAP polyclonal antibody 6048

Name: chicken anti-GFAP polyclonal antibody 6048
SKU: 6048parent
Availability: InStock
Rating: 4.69 (122 reviews)

Rated 4.69 out of 5 based on 122 customer ratings

$100.00 – $2,600.00

Antibody summary

Chicken polyclonal to GFAP
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgY
100 µg, 25 µg, 1 mg

available sizes

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SKU: 6048parent Categories: antibody, cell marker Tag: GFAP

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	chicken
isotype	IgY
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

chicken anti-GFAP polyclonal antibody 6048

antibody
Database link: human P14136 mouse P03995 rat P47819
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:5000 IF/ICC and IHC: 1:1000-1:5000
Immunogen Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgY
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control Chicken polyclonal - Isotype Control

target relevance
Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders. Click for more on: cell markers and GFAP
Protein names Glial fibrillary acidic protein (GFAP)
Gene names GFAP,GFAP
Protein family Intermediate filament family
Mass 49880Da
Function FUNCTION: GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subellular location SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269\|PubMed:12058025}. Note=Associated with intermediate filaments. {ECO:0000269\|PubMed:12058025}.
Tissues TISSUE SPECIFICITY: Expressed in cells lacking fibronectin. {ECO:0000269\|PubMed:1847665}.
Structure SUBUNIT: Interacts with SYNM. {ECO:0000250\|UniProtKB:P03995}.; SUBUNIT: [Isoform 2]: Interacts with PSEN1 (via N-terminus). {ECO:0000269\|PubMed:12058025}.
Post-translational modification PTM: Phosphorylated by PKN1. {ECO:0000269\|PubMed:12686604, ECO:0000269\|PubMed:9099667, ECO:0000269\|PubMed:9175763}.
Involvement in disease DISEASE: Alexander disease (ALXDRD) [MIM:203450]: A rare disorder of the central nervous system. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death within the first decade. Infants with Alexander disease develop a leukodystrophy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Histologically, Alexander disease is characterized by Rosenthal fibers, homogeneous eosinophilic inclusions in astrocytes. {ECO:0000269\|PubMed:11138011, ECO:0000269\|PubMed:11567214, ECO:0000269\|PubMed:11595337, ECO:0000269\|PubMed:12034785, ECO:0000269\|PubMed:12034796, ECO:0000269\|PubMed:12581808, ECO:0000269\|PubMed:12944715, ECO:0000269\|PubMed:12975300, ECO:0000269\|PubMed:15030911, ECO:0000269\|PubMed:15732097, ECO:0000269\|PubMed:17043438, ECO:0000269\|PubMed:17805552, ECO:0000269\|PubMed:17894839, ECO:0000269\|PubMed:17934883, ECO:0000269\|PubMed:17960815, ECO:0000269\|PubMed:18004641, ECO:0000269\|PubMed:18079314, ECO:0000269\|PubMed:19412928, ECO:0000269\|PubMed:20359319, ECO:0000269\|PubMed:21917775, ECO:0000269\|PubMed:23364391, ECO:0000269\|PubMed:23743246, ECO:0000269\|PubMed:24742911}. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P14136
The UniProt Consortium

Data

Immunofluorescent analysis of a section of mouse hippocampus stained with chicken pAb to GFAP, 6048, dilution 1:5,000 in green and costained with rabbit pAb to FOX3/NeuN, 2615, dilution 1:5,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion with 4% paraformaldehyde, mouse brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with the above antibodies. The GFAP antibody stains a network of astroglial cells while the Fox3/NeuN antibody stains the nuclei and proximal perikarya of neurons.

Immunofluorescent analysis of cortical neuron-glial cell culture from E20 rat costained with chicken pAb to Glial Fibrillary Acidic Protein-GFAP, 6048, dilution 1:2,000 in red, and mouse mAb to MAP-? (tau) 40680, dilution 1:2,000 in green. The blue is DAPI staining of nuclear DNA. The GFAP labels astroglial cells, while the MAP-? antibody stains neuronal cell perikarya, dendrites and axons.

Above is an image made by Dr. Fatimeh Shaerzadeh, working in the lab of Professor Habibeh Khoshbouei in the McKnight Brain Institute. A section of mouse frontal cerebral cortex was stained with EnCor chicken polyclonal antibody to GFAP 6048 in green and DNA in blue. The fibrous component of the processes of astroctyes are clearly revealed. Mouse select on image for larger view.

Chromogenic Immunostaining of a formalin fixed paraffin embedded human cerebellum section with chicken pAb to GFAP, 6048, dilution 1:20,000, detected in DAB (brown) following the 8718C method. Hematoxylin (blue) was used as the counterstain. The GFAP antibody detects the core of processes of astrocytes and Bergman glia within the granular and molecular layers.

Western blot analysis of whole brain lysates using chicken pAb to GFAP, 6048, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] mouse brain. The strong band at about 50 kDa corresponds to the GFAP protein. Smaller proteolytic fragments and alternate transcripts of GFAP may also be detected on such blots.

Publications

pmid	title	authors	citation
36828035	WWOX P47T partial loss-of-function mutation induces epilepsy, progressive neuroinflammation, and cerebellar degeneration in mice phenocopying human SCAR12.	Tabish Hussain, Kevin Sanchez, Jennifer Crayton, Dhurjhoti Saha, Collene Jeter, Yue Lu, Martin Abba, Ryan Seo, Jeffrey L Noebels, Laura Fonken, C Marcelo Aldaz	Prog Neurobiol 223:102425
36640336	Neuronal and astrocytic contributions to Huntington's disease dissected with zinc finger protein transcriptional repressors.	Mohitkumar R Gangwani, Joselyn S Soto, Yasaman Jami-Alahmadi, Srushti Tiwari, Riki Kawaguchi, James A Wohlschlegel, Baljit S Khakh	Cell Rep 42:111953
36633130	ASK1 inhibitor NQDI?1 decreases oxidative stress and neuroapoptosis via the ASK1/p38 and JNK signaling pathway in early brain injury after subarachnoid hemorrhage in rats.	Jiajia Duan, Wen Yuan, Juan Jiang, Jikai Wang, Xiaoxin Yan, Fei Liu, Aihua Liu	Mol Med Rep 27:N/A
36614107	Cerebral Folate Metabolism in Post-Mortem Alzheimer's Disease Tissues: A Small Cohort Study.	Naila Naz, Syeda F Naqvi, Nadine Hohn, Kiara Whelan, Phoebe Littler, Federico Roncaroli, Andrew C Robinson, Jaleel A Miyan	Int J Mol Sci 24:N/A
36593462	Intracellular deposits of amyloid-beta influence the ability of human iPSC-derived astrocytes to support neuronal function.	Evangelos Konstantinidis, Benjamin Portal, Tobias Mothes, Chiara Beretta, Maria Lindskog, Anna Erlandsson	J Neuroinflammation 20:3

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.