| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | chicken |
| isotype | IgY |
| clonality | polyclonal |
| concentration | 1 mg/mL |
| applications | ELISA |
| available sizes | 1 mg, 100 µg, 25 µg, 25 µL |
chicken anti-BrdU polyclonal antibody 9012
$100.00 – $2,600.00
Antibody summary
- Chicken polyclonal to BrdU
- Suitable for: ELISA
- Reacts with: tagged fusion proteins
- Isotype: IgY
- 100 µg, 25 µg, 1mg
chicken anti-BrdU polyclonal antibody 9012
| antibody |
|---|
| Database link: BrdU tag |
| Tested applications ELISA |
| Recommended dilutions user optimized |
| Immunogen Bromodeoxyuridine (BrdU) coupled to KLH |
| Size and concentration 25, 100, 1000µg and 1 mg/mL |
| Form liquid |
| Storage Instructions 2-8°C |
| Storage buffer PBS, pH 7.2, 0.09% NaN3 |
| Purity affinity purified |
| Clonality polyclonal |
| Isotype IgY |
| Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723 |
| Isotype control Chicken polyclonal - Isotype Control |
| target relevance |
|---|
| 5-bromo-2'-deoxyuridine (BrdU) is a thymidine analog commonly utilized in cell proliferation studies. As a synthetic nucleotide, BrdU is incorporated into the DNA of actively dividing cells during the S phase of the cell cycle. This property allows BrdU to be utilized as a marker for actively proliferating cells. Researchers can administer BrdU to cell cultures or living organisms and subsequently detect the presence of BrdU-labeled cells using specific antibodies targeting BrdU. Immunohistochemistry or immunofluorescence techniques employing BrdU antibodies enable the visualization and quantification of proliferating cells, providing valuable information on cell proliferation rates, tissue growth, and turnover. BrdU-based assays have been widely used in various fields, including developmental biology, cancer research, and regenerative medicine, to investigate cellular kinetics and to understand the dynamics of tissue regeneration and repair. Click for more on: cell markers and BrdU |
| Biotechnology 5-bromo-2-deoxyuridine (BrdU) has proven to be a valuable marker for studying cell proliferation in various research fields, including cancer biology, developmental biology, and neuroscience. BrdU is a thymidine analog that incorporates into the DNA of actively dividing cells during the S phase of the cell cycle. As a result, it becomes integrated into newly synthesized DNA strands, making it a specific and reliable indicator of cell proliferation. By administering BrdU to experimental models, researchers can trace and identify proliferating cells over specific timeframes, providing valuable insights into cell turnover rates, tissue regeneration, and growth patterns. Utilizing BrdU in immunohistochemistry or flow cytometry allows scientists to visualize and quantify the proportion of dividing cells in tissues or cultures, aiding in the assessment of cell proliferation rates and the effects of various experimental treatments or genetic manipulations. BrdU???s versatility and sensitivity as a cell proliferation marker have significantly advanced our understanding of cellular dynamics and have contributed to significant discoveries in biological research. |
Data
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Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 6 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
| pmid | title | authors | citation |
|---|---|---|---|
| 37333084 | otpb functions in a Lef1-dependent transcriptional network required for expression of the stress response inhibitor crhbp in the zebrafish hypothalamus | Figueroa P, Cheng J, Wang G, Kartchner C, Brito de Andrade D, Watters H, Crispell E, Dorsky RI. | bioRxiv [Preprint]. 2023 Jun 9:2023.06.07.544119. doi: 10.1101/2023.06.07.544119. |
| 28837622 | Lef1-dependent hypothalamic neurogenesis inhibits anxiety | Xie Y, Kaufmann D, Moulton MJ, Panahi S, Gaynes JA, Watters HN, Zhou D, Xue HH, Fung CM, Levine EM, Letsou A, Brennan KC, Dorsky RI. | PLoS Biol. 2017 Aug 24;15(8):e2002257. doi: 10.1371/journal.pbio.2002257. eCollection 2017 Aug. |
| 26774784 | Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation | McPherson AD, Barrios JP, Luks-Morgan SJ, Manfredi JP, Bonkowsky JL, Douglass AD, Dorsky RI. | Curr Biol. 2016 Jan 25;26(2):263-269. doi: 10.1016/j.cub.2015.11.064. Epub 2016 Jan 7. |
| 26603385 | Hypothalamic radial glia function as self-renewing neural progenitors in the absence of Wnt/β-catenin signaling | Duncan RN, Xie Y, McPherson AD, Taibi AV, Bonkowsky JL, Douglass AD, Dorsky RI. | Development. 2016 Jan 1;143(1):45-53. doi: 10.1242/dev.126813. Epub 2015 Nov 24. |
| 25888075 | Wnt/ß-catenin signaling is required for radial glial neurogenesis following spinal cord injury | Briona LK, Poulain FE, Mosimann C, Dorsky RI. | Dev Biol. 2015 Jul 1;403(1):15-21. doi: 10.1016/j.ydbio.2015.03.025. Epub 2015 Apr 14. |
Protocols
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| ELISA |
Documents
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