chicken anti-BrdU polyclonal antibody 9012

$100.00 – $2,600.00

Antibody summary

Chicken polyclonal to BrdU
Suitable for: ELISA
Reacts with: tagged fusion proteins
Isotype: IgY
100 µg, 25 µg, 1mg

available sizes

Clear

SKU: 9012parent Categories: antibody, cell marker Tag: BrdU

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	chicken
isotype	IgY
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

chicken anti-BrdU polyclonal antibody 9012

antibody
Database link: BrdU tag
Tested applications ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with BrdU as determined by blotting and ELISA techniques. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen Bromodeoxyuridine (BrdU) coupled to KLH
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgY
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control Chicken polyclonal - Isotype Control

target relevance

5-bromo-2'-deoxyuridine (BrdU) is a thymidine analog commonly utilized in cell proliferation studies. As a synthetic nucleotide, BrdU is incorporated into the DNA of actively dividing cells during the S phase of the cell cycle. This property allows BrdU to be utilized as a marker for actively proliferating cells. Researchers can administer BrdU to cell cultures or living organisms and subsequently detect the presence of BrdU-labeled cells using specific antibodies targeting BrdU. Immunohistochemistry or immunofluorescence techniques employing BrdU antibodies enable the visualization and quantification of proliferating cells, providing valuable information on cell proliferation rates, tissue growth, and turnover. BrdU-based assays have been widely used in various fields, including developmental biology, cancer research, and regenerative medicine, to investigate cellular kinetics and to understand the dynamics of tissue regeneration and repair.

Click for more on: cell markers and BrdU

Biotechnology
5-bromo-2-deoxyuridine (BrdU) has proven to be a valuable marker for studying cell proliferation in various research fields, including cancer biology, developmental biology, and neuroscience. BrdU is a thymidine analog that incorporates into the DNA of actively dividing cells during the S phase of the cell cycle. As a result, it becomes integrated into newly synthesized DNA strands, making it a specific and reliable indicator of cell proliferation. By administering BrdU to experimental models, researchers can trace and identify proliferating cells over specific timeframes, providing valuable insights into cell turnover rates, tissue regeneration, and growth patterns. Utilizing BrdU in immunohistochemistry or flow cytometry allows scientists to visualize and quantify the proportion of dividing cells in tissues or cultures, aiding in the assessment of cell proliferation rates and the effects of various experimental treatments or genetic manipulations. BrdU???s versatility and sensitivity as a cell proliferation marker have significantly advanced our understanding of cellular dynamics and have contributed to significant discoveries in biological research.

Data

ELISA results with anti-BrdU antibody 9012

Publications

pmid	title	authors	citation
37333084	otpb functions in a Lef1-dependent transcriptional network required for expression of the stress response inhibitor crhbp in the zebrafish hypothalamus.	Priscilla Figueroa, Jennifer Cheng, Guangning Wang, Cade Kartchner, Debora Brito de Andrade, Harrison Watters, Ethan Crispell, Richard I Dorsky	bioRxiv N/A:N/A
28837622	Lef1-dependent hypothalamic neurogenesis inhibits anxiety.	Yuanyuan Xie, Dan Kaufmann, Matthew J Moulton, Samin Panahi, John A Gaynes, Harrison N Watters, Dingxi Zhou, Hai-Hui Xue, Camille M Fung, Edward M Levine, Anthea Letsou, K C Brennan, Richard I Dorsky	PLoS Biol 15:e2002257
26774784	Motor Behavior Mediated by Continuously Generated Dopaminergic Neurons in the Zebrafish Hypothalamus Recovers after Cell Ablation.	Adam D McPherson, Joshua P Barrios, Sasha J Luks-Morgan, John P Manfredi, Joshua L Bonkowsky, Adam D Douglass, Richard I Dorsky	Curr Biol 26:263-269
26603385	Hypothalamic radial glia function as self-renewing neural progenitors in the absence of Wnt/?-catenin signaling.	Robert N Duncan, Yuanyuan Xie, Adam D McPherson, Andrew V Taibi, Joshua L Bonkowsky, Adam D Douglass, Richard I Dorsky	Development 143:45-53
25888075	Wnt/�-catenin signaling is required for radial glial neurogenesis following spinal cord injury.	Lisa K Briona, Fabienne E Poulain, Christian Mosimann, Richard I Dorsky	Dev Biol 403:15-21

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.