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FLAG/dyddddk antibodies
The DYDDDDK tag, also known as the FLAG tag, is a short peptide sequence that has become a popular and powerful tool in recombinant protein expression. When fused to the N- or C-terminus of a target protein, the DYDDDDK tag allows for easy detection, purification, and immunoprecipitation of the expressed protein. Its small size and minimal impact on protein folding and function make it an attractive choice for various research applications. The DYDDDDK tag is recognized by commercially available high-affinity antibodies, facilitating the sensitive and specific detection of the tagged protein using techniques like Western blotting or immunofluorescence. Furthermore, the FLAG tag is amenable to purification via affinity chromatography, where anti-FLAG antibodies are immobilized on a resin, enabling efficient and rapid purification of the tagged protein from complex biological samples.
ICC/IF with antibody [4849] : Immunofluorescence cell staining- rabbit anti-DYDDDDK tag polyclonal antibody 4849
Immunofluorescent analysis of paraformaldehyde (PFA) fixed, permeabilized with 0.1% triton X-100 CHO cells transfected with GFP-3XDYKDDDK construct (CHO-GFP-2xFLAG) labeling DYKDDDDK tag with antibody #4849 at 5µg/mL, followed by Donkey Anti-rabbit IgG, Alexa 594 conjugated with 2.5µg/mL (red). Nucleus was counterstained with DAPI (blue).
WB with antibody [5299] : 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2).
ICC/IF with antibody [8963] : Monoclonal Mouse anti-FLAG-Tag Antibody recognizes N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins. Validated for use in Western Blot and ELISA assays.
