ICC/IF image goat anti MAP2 polyclonal antibody 8718

goat anti-MAP2 polyclonal antibody 8718

$100.00 – $2,600.00

Antibody summary

Goat polyclonal to MAP2
Suitable for: WB, ICC/IF
Reacts with: human, mouse, rat
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

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SKU: 8718parent Categories: antibody, cell marker Tag: MAP2

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	goat
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

goat anti-MAP2 polyclonal antibody 8718

antibody
Database link: human P11137 mouse P20357 rat P15146
Tested applications WB,ICC/IF
Recommended dilutions WB: 1:2000 IF/ICC and IHC: 1:1000-1:2000
Immunogen Recombinant human projection domain sequences, amino acids 377-1505, EnCor products Prot-r-MAP2-P1, Prot-r-MAP2-P2 and Prot-r-MAP2-P3.
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control Goat polyclonal - Isotype Control

target relevance
As a key component of the cytoskeleton in neurons, MAP2 plays a critical role in regulating microtubule dynamics and stabilizing neuronal processes such as dendrites and axons. Its abundant presence in these structures makes it an essential factor in establishing and maintaining the intricate neuronal morphology required for proper cell function and connectivity in the nervous system. Researchers have focused on studying MAP2 to gain deeper insights into neuronal development, synaptic plasticity, and neurodegenerative diseases, as its expression and phosphorylation are closely associated with synaptic maturation and neuronal plasticity. As a cell marker, MAP2 serves as an invaluable marker for identifying neuronal differentiation and assessing neuronal damage or degeneration. This antibody recognizes MAP2 in neuronal cells and can be used as a cell marker antibody when used with a suitable secondary antibody. Click for more on: cell markers and MAP2
Protein names Microtubule-associated protein 2 (MAP-2)
Gene names MAP2,MAP2
Mass 199526Da
Function The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Subellular location Cytoplasm, cytoskeleton Cell projection, dendrite
Structure Interacts with KNDC1 (via KIND2); the interaction enhances MAP2 phosphorylation and localizes KNDC1 to dendrites. Interacts with DPYSL5 (PubMed:33894126).
Post-translational modification Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. The interaction with KNDC1 enhances MAP2 threonine phosphorylation (By similarity).
Target Relevance information above includes information from UniProt accession: P11137
The UniProt Consortium

Data

Immunofluorescent analysis of rat brain stem section stained with goat pAb to MAP2 dilution 1:2,000 in red, and costained with mouse mAb to MBP, dilution 1:5,000, in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The antibody labels MAP2 protein in the perikarya and dendrites of the most neurons, notably motorneurons in the brain stem, and the MBP antibody stains myelin sheath around axons.

Chromogenic immunostaining of a formalin fixed paraffin embedded rat hippocampus section with goat pAb to MAP2 dilution 1:2,000, detected with DAB (brown) using the Vector Elite 8718C-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. The antibody labels the MAP2 protein in the somata and dendrites of most neurons.

Western blot analysis of whole brain lysates using goat pAb to MAP2 dilution 1:5,000 in green: [1] protein standard (red), [2] rat, and [3] mouse brain lysate. A band at about 280kDa corresponds to the MAP2A and MAP2B proteins.

Publications

pmid	title	authors	citation
35978188	Endocytosis in the axon initial segment maintains neuronal polarity.	Kelsie Eichel, Takeshi Uenaka, Vivek Belapurkar, Rui Lu, Shouqiang Cheng, Joseph S Pak, Caitlin A Taylor, Thomas C S�dhof, Robert Malenka, Marius Wernig, Engin �zkan, David Perrais, Kang Shen	Nature 609:128-135
22623668	An ankyrinG-binding motif is necessary and sufficient for targeting Nav1.6 sodium channels to axon initial segments and nodes of Ranvier.	Andreas Gasser, Tammy Szu-Yu Ho, Xiaoyang Cheng, Kae-Jiun Chang, Stephen G Waxman, Matthew N Rasband, Sulayman D Dib-Hajj	J Neurosci 32:7232-43
18988710	Gliotypic neural stem cells transiently adopt tumorigenic properties during normal differentiation.	Noah M Walton, Gregory E Snyder, Donghyun Park, Firas Kobeissy, Bjorn Scheffler, Dennis A Steindler	Stem Cells 27:280-9
16832065	Temporally restricted substrate interactions direct fate and specification of neural precursors derived from embryonic stem cells.	A Katrin Goetz, Bjorn Scheffler, Huan-Xin Chen, Shanshan Wang, Oleg Suslov, Hui Xiang, Oliver Br�stle, Steve N Roper, Dennis A Steindler	Proc Natl Acad Sci U S A 103:11063-8
16817858	Differential modulation of sodium channel Na(v)1.6 by two members of the fibroblast growth factor homologous factor 2 subfamily.	Anthony M Rush, Ellen K Wittmack, Lynda Tyrrell, Joel A Black, Sulayman D Dib-Hajj, Stephen G Waxman	Eur J Neurosci 23:2551-62

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.