Additional information
Mikrogen recomLine Chlamydia tests are serological, qualitative in vitro tests that allow the determination
of the immune response of all three human pathogenic Chlamydia species in a single assay. They consist of
different species-specific, recombinantly produced antigens on nitrocellulose strips that can be used to
determine IgG, IgA, and IgM antibodies against immunodominant C. trachomatis, C. pneumoniae,
and C. psittaci antigens.
The recomLine Chlamydia can be used as a confirmatory test but also as a screening test. Advantages- Immune status of a possible C. trachomatis, C. pneumoniae, and C. psittaci infection can be confirmed in one preparation and on one strip.
- Helpful for in vitro fertilization diagnostics due to the species-specific antigens MOMP, TARP, CPAF, and HSP60 of C. trachomatis.
- Highly specific detection of C. pneumoniae due to the use of species-specific antigens, e.g., YwbM.
- Only assay for specific serological C. psittaci diagnostics.
- Can be used as both screening and confirmatory assay.
- Separate detection of IgG, IgA, and IgM antibodies.
- Simple and clear interpretation due to easy-to-read banding.
- Partial and full automation, software-based evaluation (recomScan), and integration with laboratory information system possible.
- Highest sensitivity and specificity through use of recombinant, species-specific antigens:
Bands| Antigen | Species | Signification |
|---|
| MOMP | C. tr., C. pn., C. ps. | "major outer membrane protein"; Immunodominant outer membrane antigen | | OMP2 | C. tr., C. pn., C. ps. | "major outer membrane protein 2"; cysteine-rich outer membrane protein; general marker for infection with Chlamydia | | TARP | C. tr., C. pn., C. ps. | "translocated actin-recruiting protein"; Binds actin | | CPAF | C. tr., C. pn., C. ps. | "chlamydial protease-like activity factor"; Virulence factor | | HSP60 | C. tr. | "heat shock protein 60"; In |
| target relevance |
|---|
Organism
Chlamydia abortus | Structure and strains
Chlamydia abortus is a species in Chlamydiota that causes abortion and fetal death in mammals, including humans. Chlamydia abortus was renamed in 1999 as Chlamydophila psittaci along with all Chlamydiota except Chlamydia trachomatis. This was based on a lack of evident glycogen production and on resistance to the antibiotic sulfadiazine. In 1999 C. psittaci and C. abortus were recognized as distinct species based on differences of pathogenicity and DNA DNA hybridization. In 2015, this new name was reverted to Chlamydia | Disease
Chlamydiae are gram-negative, obligatory intracellular bacteria. Characteristically, the chlamydial cell wall lacks a peptidoglycan layer. Only the species Chlamydia trachomatis, Chlamydia pneumoniae (Chlamydophila pneumoniae) and Chlamydia psittaci (also referred to as Chlamydophila psittaci) are relevant to human disease.
Infections with Chlamydia can affect the mucous membranes in the eye, airways and genitals and may lead to serious damage. Chlamydia trachomatis infection is the most common sexually transmitted bacterial disease in Europe and may result in ectopic pregnancy, premature birth, sterility and arthritis. Untreated chlamydioses are the primary cause of blindness in developing countries. Around 5 to 15% of all cases of pneumonia contracted outside of hospitals are due to infection with Chlamydia pneumoniae transmitted through the air by droplets from person to person. Older people and those with weakened immune systems are particularly susceptible. Chlamydia psittaci is the causative agent of ornithosis (Psittacosis) which occurs worldwide. The natural reservoirs are birds. Transmission occurs by inhalation of aerosols or dust containing the organism. Following an incubation time of around six to 20 days ornithosis manifests as atypical bronchopneumonia with flu-like symptoms, but other organs may also be afflicted. Diagnosis | Detection and diagnosis
Approximately two weeks after the first symptoms of a primary infection an increase of the IgA and IgM antibody activity occurs, which peaks after five weeks and usually declines by the 10th week. The IgG antibody activity reaches a maximum around week twelve and remains detectable for several years. In cases of reinfection IgA and IgG antibodies reappear rapidly. |
|
Reviews
There are no reviews yet.