Immunohistochemistry (IHC) : Human ovarian carcinoma stained with anti-CA125 antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.

mouse anti-CA 125/ MUC16 monoclonal antibody (ZM53) 6043

$160.00 – $528.00

Antibody summary

Mouse monoclonal to CA 125/ MUC16
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1
Control: Ovarian carcinoma
Visualization: Extracellular membrane-associated
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6043parent Category: antibody Tags: anatomy, CA 125/ MUC16, Gynecological Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-CA 125/ MUC16 monoclonal antibody ZM53 6043

antibody
Database link: human Q8WXI7
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Ovarian carcinoma
Immunogen Full length native CA125 protein purified from human ovarian carcinoma
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Mucin-16 (MUC-16) (Ovarian cancer-related tumor marker CA125) (CA-125) (Ovarian carcinoma antigen CA125)
Gene names MUC16,MUC16 CA125
Mass 1519175Da
Function Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Subellular location Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. Note=May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.
Tissues Expressed in corneal and conjunctival epithelia (at protein level). Overexpressed in ovarian carcinomas and ovarian low malignant potential (LMP) tumors as compared to the expression in normal ovarian tissue and ovarian adenomas.
Structure Binds to MSLN. Binding to MSLN mediates heterotypic cell adhesion. This may contribute to the metastasis of ovarian cancer to the peritoneum by initiating cell attachment to the mesothelial epithelium via binding to MSLN.
Post-translational modification Heavily O-glycosylated; expresses both type 1 and type 2 core glycans.; Heavily N-glycosylated; expresses primarily high mannose and complex bisecting type N-linked glycans.; May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space.; May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
Domain TOPO_DOM 1
Target Relevance information above includes information from UniProt accession: Q8WXI7
The UniProt Consortium

Data

Human ovarian carcinoma stained with anti-CA125 antibody using peroxidase-conjugate and DAB chromogen. Note cell membrane staining of tumor cells.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.