Migration Assay: Cells expressing recombinant CCR5 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MIP-1α. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

Biotinylated Macrophage inflammatory proteins 1-a (MIP1a) 8017

$134.00 – $3,368.00

Summary

Expression: E.coli
Amino Acid Range: 24-92

SKU: 8017parent Categories: chemokine, proteins Tag: CCL3 (MIP-1a)

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	P10147
express system	E.coli
product tag	biotin at C-terminal
purity	> 97% by SDS PAGE
molecular weight	Predicted Molecular Mass: 10,237.4973 Da Extinction Coefficient: 18,020 M-1 cm-1 Actual Molecular Mass: 10,237.4973 Da by ESI Mass Spec
available size	100 µg, 20 µg, 5 µg, 50 µg
endotoxin	<0.01 EU per 1μg of the protein by the LAL method

Biotinylated Macrophage inflammatory proteins 1-a (MIP1a) 8017

protein
Database link: human P10147
Size and concentration 2, 10, 50, 100µg and lyophilized
Form Lyophilized
Storage Instructions Avoid repeated freeze-thaw cycles: • 12 months from date of receipt, -20 to -70 °C as supplied. • 1 month, 2 to 8 °C under sterile conditions after reconstitution. • 3 months, -20 to -70 °C under sterile conditions after reconstitution
Storage buffer â€‹Reconstitution: Spin sample prior to reconstitution. Recommended concentration of 100µg/mL in sterile water. Shipping: Room Temp
Purity > 97% by SDS PAGE and HPLC

target relevance
Monocyte protein 3 (MCP-3/CCL7) is secreted by monocytes and certain tumor cell lines. It is a chemoattractant for monocytes and other leukocytes. MCP-3 binds and signals through several chemokine receptors including CCR1, CCR2, CCR3, and appears to be an antagonist for CCR5.Biotinylated CCL7 is made using the enzymatic method, which has several advantages over chemical biotinylation methods. The attachment of biotin at a specific lysine residue leads to a modified chemokine with functionalities comparable to those of the unmodified CCL7 in migration assay. Combining with avidin analogues conjugated to various fluorescent labels, biotinylated CCL7 is useful in studies on receptor identification, distribution, chemokine binding, and other cellular assays. They serve as great tools in visualization and quantification, and replace the needs for radioactively labeled chemokines.
Protein names C-C motif chemokine 3 (G0/G1 switch regulatory protein 19-1) (Macrophage inflammatory protein 1-alpha) (MIP-1-alpha) (PAT 464.1) (SIS-beta) (Small-inducible cytokine A3) (Tonsillar lymphocyte LD78 alpha protein) [Cleaved into: MIP-1-alpha(4-69) (LD78-alpha(4-69))]
Gene names CCL3,CCL3 G0S19-1 MIP1A SCYA3
Protein family Intercrine beta (chemokine CC) family
Mass 10085Da
Function Monokine with inflammatory and chemokinetic properties. Binds to CCR1, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-alpha induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV).
Subellular location Secreted.
Structure Self-associates. Also heterodimer of MIP-1-alpha(4-69) and MIP-1-beta(3-69) (PubMed:12070155). Interacts with CCR1 (PubMed:15905581).
Post-translational modification N-terminal processed form LD78-alpha(4-69) is produced by proteolytic cleavage after secretion from HTLV1-transformed T-cells.
Target Relevance information above includes information from UniProt accession: P10147
The UniProt Consortium

Data

Migration Assay: Cells expressing recombinant CCR5 were assayed for migration through a transwell filter at various concentrations of WT or Biotinylated MIP-1α. Responses are expressed as the % of total input cells (Blue: wild type; Red: biotinylated).

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.