Immunohistochemistry (IHC) : Formalin-fixed, paraffin-embedded human colon carcinoma stained with anti-CEACAM5 polyclonal antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and luminal surface staining of tumor cells

rabbit anti-CEACAM5 monoclonal antibody (ZR370) 6122

$160.00 – $528.00

Antibody summary

Rabbit monoclonal to CEACAM5
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG
Control: Colon carcinoma
Visualization: Cytoplasmic and luminal membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6122parent Category: antibody Tags: anatomy, CEACAM5, GI Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

rabbit anti-CEACAM5 monoclonal antibody ZR370 6122

antibody
Database link: human P06731
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Colon carcinoma
Immunogen Recombinant full-length human CEA protein
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG
Compatible secondaries goat anti-rabbit IgG, H&L chain specific, peroxidase conjugated, conjugated polyclonal antibody 9512 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody 2079 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody 7863 goat anti-rabbit IgG, H&L chain specific, Cross Absorbed polyclonal antibody 2371 goat anti-rabbit IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1715 goat anti-rabbit IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1720
Isotype control Rabbit polyclonal - Isotype Control

target relevance
Protein names Carcinoembryonic antigen-related cell adhesion molecule 5 (Carcinoembryonic antigen) (CEA) (Meconium antigen 100) (CD antigen CD66e)
Gene names CEACAM5,CEACAM5 CEA
Protein family Immunoglobulin superfamily, CEA family
Mass 9606Da
Function Cell surface glycoprotein that plays a role in cell adhesion, intracellular signaling and tumor progression (PubMed:10864933, PubMed:10910050, PubMed:2803308). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM6 (PubMed:2803308). Plays a role as an oncogene by promoting tumor progression; induces resistance to anoikis of colorectal carcinoma cells (PubMed:10910050).; (Microbial infection) Receptor for E.coli Dr adhesins. Binding of E.coli Dr adhesins leads to dissociation of the homodimer.
Subellular location Cell membrane ; Lipid-anchor, GPI-anchor. Apical cell membrane. Cell surface. Note=Localized to the apical glycocalyx surface.
Tissues Expressed in columnar epithelial and goblet cells of the colon (at protein level) (PubMed:10436421). Found in adenocarcinomas of endodermally derived digestive system epithelium and fetal colon.
Structure Homodimer.
Post-translational modification Complex immunoreactive glycoprotein with a MW of 180 kDa comprising 60% carbohydrate.
Target Relevance information above includes information from UniProt accession: P06731
The UniProt Consortium

Data

Formalin-fixed, paraffin-embedded human colon carcinoma stained with anti-CEACAM5 polyclonal antibody using peroxidase-conjugate and DAB chromogen. Note cytoplasmic and luminal surface staining of tumor cells

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.