Immunohistochemistry (IHC) : High grade cervical intraepithelial neoplasia (CINIII) stained with anti-p16 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear and cytoplasmic staining of dysplastic cells, whereas the normal squamoujs mucosa is negative.

mouse anti-p16INK4a monoclonal antibody (G175-405) 6303

$160.00 – $528.00

Antibody summary

Mouse monoclonal to p16INK4a
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG1 /λ
Control: Cervical squamous cell carcinoma
Visualization: Cytoplasmic and nuclear
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6303parent Category: antibody Tags: anatomy, Gynecological Pathology, p16INK4a

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG1 /λ
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-p16INK4a monoclonal antibody G175-405 6303

antibody
Database link: human P42771
Tested applications IHC
Recommended dilutions As directed
Immunogen A synthetic peptide from the C-terminus of human p16INK4a
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG1
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG1 - Isotype Control

target relevance
Protein names Cyclin-dependent kinase inhibitor 2A (Cyclin-dependent kinase 4 inhibitor A) (CDK4I) (Multiple tumor suppressor 1) (MTS-1) (p16-INK4a) (p16-INK4) (p16INK4A)
Protein family CDKN2 cyclin-dependent kinase inhibitor family
Mass 16533Da
Function Acts as a negative regulator of the proliferation of normal cells by interacting strongly with CDK4 and CDK6. This inhibits their ability to interact with cyclins D and to phosphorylate the retinoblastoma protein.
Subellular location Cytoplasm . Nucleus .
Tissues Widely expressed but not detected in brain or skeletal muscle. Isoform 3 is pancreas-specific.
Structure Heterodimer with CDK4 or CDK6. Predominant p16 complexes contained CDK6. Interacts with CDK4 (both 'T-172'-phosphorylated and non-phosphorylated forms); the interaction inhibits cyclin D-CDK4 kinase activity. Interacts with ISCO2.
Post-translational modification PTM: Phosphorylation seems to increase interaction with CDK4.
Involvement in disease Note=The association between cutaneous and uveal melanomas in some families suggests that mutations in CDKN2A may account for a proportion of uveal melanomas. However, CDKN2A mutations are rarely found in uveal melanoma patients.; Melanoma, cutaneous malignant 2 (CMM2) [MIM:155601]: A malignant neoplasm of melanocytes, arising de novo or from a pre-existing benign nevus, which occurs most often in the skin but may also involve other sites. Note=Disease susceptibility is associated with variants affecting the gene represented in this entry.; Familial atypical multiple mole melanoma-pancreatic carcinoma syndrome (FAMMMPC) [MIM:606719]: An inherited cancer predisposition syndrome characterized by an increased risk of developing malignant melanoma and/or pancreatic cancer. Mutation carriers within families may develop either or both types of cancer. Note=The disease is caused by variants affecting the gene represented in this entry.; Melanoma-astrocytoma syndrome (MASTS) [MIM:155755]: Characterized by a dual predisposition to melanoma and neural system tumors, commonly astrocytoma. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P42771
The UniProt Consortium

Data

High grade cervical intraepithelial neoplasia (CINIII) stained with anti-p16 antibody using peroxidase-conjugate and DAB chromogen. Note the nuclear and cytoplasmic staining of dysplastic cells, whereas the normal squamoujs mucosa is negative.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.