Immunohistochemistry (IHC) : Human colon carcinoma stained with anti-keratin 20 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of carcinoma glands.

mouse anti-Cytokeratin 20 monoclonal antibody (ZM42) 6142

$160.00 – $528.00

Antibody summary

Mouse monoclonal to Cytokeratin 20
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgG2b
Control: Colon carcinoma
Visualization: Cytoplasmic
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

SKU: 6142parent Category: antibody Tags: anatomy, Cytokeratin 20, GI Pathology

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgG2b
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-Cytokeratin 20 monoclonal antibody ZM42 6142

antibody
Database link: human P35900
Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Applicaiton Notes Positive control: Colon carcinoma
Immunogen Recombinant human KRT20 protein fragment (around aa 196-323)
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgG2b
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgG2b - Isotype Control

target relevance
Protein names Keratin, type I cytoskeletal 20 (Cytokeratin-20) (CK-20) (Keratin-20) (K20) (Protein IT)
Protein family Intermediate filament family
Mass 48487Da
Function Plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, plays a role in the secretion of mucin in the small intestine (By similarity).
Subellular location Cytoplasm .
Tissues Expressed predominantly in the intestinal epithelium. Expressed in luminal cells of colonic mucosa. Also expressed in the Merkel cells of keratinized oral mucosa; specifically at the tips of some rete ridges of the gingival mucosa, in the basal layer of the palatal mucosa and in the taste buds of lingual mucosa.
Structure Heterotetramer of two type I and two type II keratins. Associates with KRT8.
Post-translational modification PTM: Hyperphosphorylation at Ser-13 occurs during the early stages of apoptosis but becomes less prominent during the later stages. Phosphorylation at Ser-13 also increases in response to stress brought on by cell injury (By similarity).; PTM: Proteolytically cleaved by caspases during apoptosis. Cleavage occurs at Asp-228.
Domain DOMAIN 70..381; /note="IF rod"; /evidence="ECO:0000255\|PROSITE-ProRule:PRU01188"
Target Relevance information above includes information from UniProt accession: P35900
The UniProt Consortium

Data

Human colon carcinoma stained with anti-keratin 20 antibody using peroxidase-conjugate and DAB chromogen. Note the cytoplasmic staining of carcinoma glands.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.