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mouse anti-Clostridium botulinum Toxin A monoclonal antibody (B364M) 1424

$620.00

Antibody summary

  • Mouse monoclonal to Clostridium botulinum Toxin A
  • Suitable for: ICC/IF,ELISA,RIA
  • Isotype: IgM
  • 500 µl
SKU: 1424parent Category: Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

mouse

isotype

IgM

clonality

monoclonal

concentration

1 mg/mL

applications

ICC/IF, WB

reactivity

Clostridium botulinum Toxin A

available sizes

500 µl

Available product – mouse anti-Clostridium botulinum Toxin A monoclonal antibody (B364M) 1424

antibody
Tested applications
ICC/IF,ELISA
Recommended dilutions
ELISA, Immunocytochemistry, Immunofluorescence

End users should determine optimal dilutions for their applications.
Immunogen
Synthetic peptide corresponding to aa 1280-1292 (Cys-Ser-Trp-Glu-Phe-Ile-Pro-Val- Asp-Asp-Gly-Trp-Gly-COOH) at the C-terminus of Clostridium botulinum Toxin A.
Size and concentration
500µL and lot specific
Form
liquid
Storage Instructions
This antibody is stable for at least one (1) year at -20° to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
Storage buffer
Hybridoma culture supernatant, cell free media.
Purity
hybridoma culture
Clonality
monoclonal
Isotype
IgM
Compatible secondaries
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486
goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854
goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706
goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716
goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control
Mouse monocolonal IgM- Isotype Control
target relevance
Protein names
Botulinum neurotoxin type A (BoNT/A) (Bontoxilysin-A) (BOTOX) [Cleaved into: Botulinum neurotoxin A light chain (LC) (EC 3.4.24.69); Botulinum neurotoxin A heavy chain (HC)]
Gene names
botA,botA bna CBO0806 CLC_0862
Protein family
Peptidase M27 family
Mass
149426Da
Function
FUNCTION: [Botulinum neurotoxin type A]: Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure (PubMed:8103915). Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles (By similarity). Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them (PubMed:19476346). Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway (By similarity). When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol (By similarity). Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release (By similarity). {ECO:0000250|UniProtKB:P0DPI0, ECO:0000269|PubMed:19476346, ECO:0000269|PubMed:8103915}.; FUNCTION: [Botulinum neurotoxin A light chain]: Has proteolytic activity (PubMed:8103915, PubMed:8294407). In vitro the whole toxin is reduced to release LC (PubMed:8103915, PubMed:8294407). After translocation into the eukaryotic host cytosol, LC hydrolyzes the 197-Gln-|-Arg-198 bond in SNAP25, blocking neurotransmitter release (PubMed:8103915, PubMed:8294407). {ECO:0000269|PubMed:8103915, ECO:0000269|PubMed:8294407, ECO:0000305|PubMed:19476346}.; FUNCTION: [Botulinum neurotoxin A heavy chain]: Responsible for host epithelial cell transcytosis, host nerve cell targeting and translocation of light chain (LC) into host cytosol. Composed of 3 subdomains; the translocation domain (TD), and N-terminus and C-terminus of the receptor-binding domain (RBD) (PubMed:9783750, PubMed:17173035). The RBD is responsible for the adherence of the toxin to the cell surface. It simultaneously recognizes 2 coreceptors; polysialated gangliosides and synaptic vesicle glycoproteins SV2A, SV2B and SV2C in close proximity on host synaptic vesicles (By similarity). The RBD specifically recognizes the N-linked glycan on 'Asn-559' of SV2A, SV2B and SV2C (By similarity). Isolated HC binds to host synaptosomes, significantly decreases uptake and toxicity of whole BoNT/A (PubMed:19476346). Binds ganglioside GD1a in vitro (PubMed:21849494). The N-terminus of the TD wraps an extended belt around the perimeter of the LC, protecting Zn(2+) in the active site; it may also prevent premature LC dissociation from the translocation channel and to protect toxin prior to translocation (PubMed:9783750). The TD inserts into synaptic vesicle membrane to allow translocation into the host cytosol (By similarity). {ECO:0000250|UniProtKB:P0DPI0, ECO:0000269|PubMed:17173035, ECO:0000269|PubMed:19476346, ECO:0000269|PubMed:21849494, ECO:0000269|PubMed:9783750}.
Catalytic activity
CATALYTIC ACTIVITY: Reaction=Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates.; EC=3.4.24.69; Evidence={ECO:0000269|PubMed:8103915, ECO:0000269|PubMed:8294407};
Subellular location
SUBCELLULAR LOCATION: [Botulinum neurotoxin A light chain]: Secreted {ECO:0000305|PubMed:9783750}. Host cytoplasm, host cytosol {ECO:0000305|PubMed:8103915, ECO:0000305|PubMed:8294407}.; SUBCELLULAR LOCATION: [Botulinum neurotoxin A heavy chain]: Secreted {ECO:0000305|PubMed:9783750}. Host synapse, host presynaptic cell membrane {ECO:0000269|PubMed:19476346}. Host cytoplasmic vesicle, host secretory vesicle, host synaptic vesicle membrane {ECO:0000305|PubMed:19476346}; Multi-pass membrane protein {ECO:0000305}.
Structure
SUBUNIT: Heterodimer; disulfide-linked heterodimer of a light chain (LC) and a heavy chain (HC) (PubMed:9783750, PubMed:17173035). Interacts with host synaptic vesicle glycoproteins SV2A, SV2B and SV2C which serve as coreceptors (By similarity). Glycosylation of 'Asn-559' in SV2C probably contributes a 12-fold increase in affinity to this interaction (By similarity). Depolarization of target tissue with high levels of K(+) leads to greater levels of receptor exposure (PubMed:19476346). {ECO:0000250|UniProtKB:P0DPI0, ECO:0000269|PubMed:17173035, ECO:0000269|PubMed:19476346, ECO:0000269|PubMed:9783750}.
Post-translational modification
PTM: In a bacterial culture the precursor chain is initally cleaved on the amino side of Gly-445 and is processed more slowly between Lys-448 and Ala-449 to give the final mature heavy chain sequence. {ECO:0000250|UniProtKB:P0DPI0}.
Target Relevance information above includes information from UniProt accession : P0DPI1
The UniProt Consortium

Data

No results found

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

pmidtitleauthorscitation

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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