| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| host | mouse |
| isotype | IgM |
| clonality | monoclonal |
| concentration | 1 mg/mL |
| applications | ICC/IF, WB |
| reactivity | Clostridium botulinum Toxin A |
| available sizes | 500 µl |
mouse anti-Clostridium botulinum Toxin A monoclonal antibody (B364M) 1424
$620.00
Antibody summary
- Mouse monoclonal to Clostridium botulinum Toxin A
- Suitable for: ICC/IF,ELISA,RIA
- Isotype: IgM
- 500 µl
mouse anti-Clostridium botulinum Toxin A monoclonal antibody (B364M) 1424
| antibody |
|---|
| Tested applications ICC/IF,ELISA |
| Recommended dilutions ELISA, Immunocytochemistry, Immunofluorescence End users should determine optimal dilutions for their applications. |
| Immunogen Synthetic peptide corresponding to aa 1280-1292 (Cys-Ser-Trp-Glu-Phe-Ile-Pro-Val- Asp-Asp-Gly-Trp-Gly-COOH) at the C-terminus of Clostridium botulinum Toxin A. |
| Size and concentration 500µL and lot specific |
| Form liquid |
| Storage Instructions This antibody is stable for at least one (1) year at -20° to -70°C. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles. |
| Storage buffer Hybridoma culture supernatant, cell free media. |
| Purity hybridoma culture |
| Clonality monoclonal |
| Isotype IgM |
| Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721 |
| Isotype control Mouse monocolonal IgM- Isotype Control |
| target relevance |
|---|
| Clostridium botulinum (strain Hall / ATCC 3502 / NCTC 13319 / Type A) BOTA Botulinum neurotoxin type A |
| Protein names Botulinum neurotoxin type A |
| Alternative names Bontoxilysin-A |
| Gene names botA |
| Protein family Belongs to the peptidase M27 family |
| Function Botulinum toxin causes flaccid paralysis by inhibiting neurotransmitter (acetylcholine) release from the presynaptic membranes of nerve terminals of the eukaryotic host skeletal and autonomic nervous system, with frequent heart or respiratory failure (PubMed:8103915). Precursor of botulinum neurotoxin A which has 2 coreceptors; complex polysialylated gangliosides found on neural tissue and specific membrane-anchored proteins of synaptic vesicles (By similarity). Receptor proteins are exposed on host presynaptic cell membrane during neurotransmitter release, when the toxin heavy chain (HC) binds to them (PubMed:19476346). Upon synaptic vesicle recycling the toxin is taken up via the endocytic pathway (By similarity). When the pH of the toxin-containing endosome drops a structural rearrangement occurs so that the N-terminus of the HC forms pores that allows the light chain (LC) to translocate into the cytosol (By similarity). Once in the cytosol the disulfide bond linking the 2 subunits is reduced and LC cleaves its target protein on synaptic vesicles, preventing their fusion with the cytoplasmic membrane and thus neurotransmitter release (By similarity) |
| Catalytic activity Limited hydrolysis of proteins of the neuroexocytosis apparatus, synaptobrevins, SNAP25 or syntaxin. No detected action on small molecule substrates. |
| Subcellular location Secreted, Host synapse, host presynaptic cell membrane, Host cytoplasmic vesicle, host secretory vesicle, host synaptic vesicle membrane |
| Structure Heterodimer; disulfide-linked heterodimer of a light chain (LC) and a heavy chain (HC) (PubMed:17173035, PubMed:9783750). Interacts with host synaptic vesicle glycoproteins SV2A, SV2B and SV2C which serve as coreceptors (By similarity). Glycosylation of 'Asn-559' in SV2C probably contributes a 12-fold increase in affinity to this interaction (By similarity). Depolarization of target tissue with high levels of K(+) leads to greater levels of receptor exposure (PubMed:19476346) |
| Post-translational modification In a bacterial culture the precursor chain is initally cleaved on the amino side of Gly-445 and is processed more slowly between Lys-448 and Ala-449 to give the final mature heavy chain sequence |
| Keywords 3D-structure, Direct protein sequencing, Disulfide bond, Host cell membrane, Host cytoplasm, Host cytoplasmic vesicle, Host membrane, Host synapse, Hydrolase, Membrane, Metal-binding, Metalloprotease, Neurotoxin, Pharmaceutical, Protease, Reference proteome, Secreted, Toxin, Transmembrane, Transmembrane helix, Virulence, Zinc |
| Sequence MPFVNKQFNYKDPVNGVDIAYIKIPNAGQMQPVKAFKIHNKIWVIPERDTFTNPEEGDLN PPPEAKQVPVSYYDSTYLSTDNEKDNYLKGVTKLFERIYSTDLGRMLLTSIVRGIPFWGG STIDTELKVIDTNCINVIQPDGSYRSEELNLVIIGPSADIIQFECKSFGHEVLNLTRNGY GSTQYIRFSPDFTFGFEESLEVDTNPLLGAGKFATDPAVTLAHELIHAGHRLYGIAINPN RVFKVNTNAYYEMSGLEVSFEELRTFGGHDAKFIDSLQENEFRLYYYNKFKDIASTLNKA KSIVGTTASLQYMKNVFKEKYLLSEDTSGKFSVDKLKFDKLYKMLTEIYTEDNFVKFFKV LNRKTYLNFDKAVFKINIVPKVNYTIYDGFNLRNTNLAANFNGQNTEINNMNFTKLKNFT GLFEFYKLLCVRGIITSKTKSLDKGYNKALNDLCIKVNNWDLFFSPSEDNFTNDLNKGEE ITSDTNIEAAEENISLDLIQQYYLTFNFDNEPENISIENLSSDIIGQLELMPNIERFPNG KKYELDKYTMFHYLRAQEFEHGKSRIALTNSVNEALLNPSRVYTFFSSDYVKKVNKATEA AMFLGWVEQLVYDFTDETSEVSTTDKIADITIIIPYIGPALNIGNMLYKDDFVGALIFSG AVILLEFIPEIAIPVLGTFALVSYIANKVLTVQTIDNALSKRNEKWDEVYKYIVTNWLAK VNTQIDLIRKKMKEALENQAEATKAIINYQYNQYTEEEKNNINFNIDDLSSKLNESINKA MININKFLNQCSVSYLMNSMIPYGVKRLEDFDASLKDALLKYIYDNRGTLIGQVDRLKDK VNNTLSTDIPFQLSKYVDNQRLLSTFTEYIKNIINTSILNLRYESNHLIDLSRYASKINI GSKVNFDPIDKNQIQLFNLESSKIEVILKNAIVYNSMYENFSTSFWIRIPKYFNSISLNN EYTIINCMENNSGWKVSLNYGEIIWTLQDTQEIKQRVVFKYSQMINISDYINRWIFVTIT NNRLNNSKIYINGRLIDQKPISNLGNIHASNNIMFKLDGCRDTHRYIWIKYFNLFDKELN EKEIKDLYDNQSNSGILKDFWGDYLQYDKPYYMLNLYDPNKYVDVNNVGIRGYMYLKGPR GSVMTTNIYLNSSLYRGTKFIIKKYASGNKDNIVRNNDRVYINVVVKNKEYRLATNASQA GVEKILSALEIPDVGNLSQVVVMKSKNDQGITNKCKMNLQDNNGNDIGFIGFHQFNNIAK LVASNWYNRQIERSSRTLGCSWEFIPVDDGWGERPL |
| UniProt accession: P0DPI1 |
Data
| No results found |
FAQ & Publications
Frequently Asked Questions
What applications has the mouse anti-Clostridium botulinum Toxin A monoclonal antibody (B364M) been validated for?
This antibody has been tested and is suitable for use in ICC/IF (Immunocytochemistry/Immunofluorescence), ELISA, and RIA applications. Users are advised to determine optimal dilutions for their specific experimental needs.
How should the mouse anti-Clostridium botulinum Toxin A monoclonal antibody be stored to maintain stability?
The antibody is stable for at least one year when stored at temperatures between -20°C and -70°C. It is recommended to store the product in appropriate aliquots to avoid multiple freeze-thaw cycles.
What is the immunogen used to generate the mouse anti-Clostridium botulinum Toxin A monoclonal antibody (B364M)?
The immunogen is a synthetic peptide corresponding to amino acids 1280-1292 at the C-terminus of Clostridium botulinum Toxin A, specifically the sequence Cys-Ser-Trp-Glu-Phe-Ile-Pro-Val-Asp-Asp-Gly-Trp-Gly-COOH.
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| relevant to this product |
|---|
| Western blot IHC ICC |
Documents
| Batch Number | QC File | SDS |
|---|---|---|
| To view batch-specific Safety Datasheets and Quality Certificates associated with your account, please Log In. | ||
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