Immunohistochemistry (IHC) : Human tonsil stained with anti-CD57 antibody using peroxidase-conjugate and DAB chromogen. Note intense cell membrane staining of natural killer cells.

mouse anti-CD57 monoclonal antibody (NK-1) 6104

$160.00 – $528.00

Antibody summary

Mouse monoclonal to CD57
Suitable for: Immunohistochemistry (formalin-fixed, paraffin-embedded tissues)
Reacts with: Human
Isotype:IgM
Control: Lymph node or tonsil
Visualization: Cell membrane
0.1, 0.5, 1.0 mL concentrated, 7 mL prediluted

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SKU: 6104parent Category: antibody Tags: anatomy, CD57, Neuropathology/Endocrine

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	mouse
isotype	IgM
clonality	monoclonal
concentration	concentrate, predilute
applications	IHC
reactivity	human
available size	0.1 mL, 0.5 mL, 1 mL concentrated, 7 mL prediluted

mouse anti-CD57 monoclonal antibody NK-1 6104

antibody

Tested applications IHC
Recommended dilutions Concentrated 1:100-200
Application Notes Positive control: Lymph node or tonsil
Immunogen Human peripheral blood mononuclear cells
Size and concentration 7 mL prediluted or 0.1, 0.5, 1.0 mL and concentrated
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Purity affinity purified
Clonality monoclonal
Isotype IgM
Compatible secondaries goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 5486 goat anti-mouse IgG, H&L chain specific, biotin conjugated, Conjugate polyclonal antibody 2685 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody 7854 goat anti-mouse IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1706 goat anti-mouse IgG, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1716 goat anti-mouse IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1721
Isotype control Mouse monoclonal IgM - Isotype Control

target relevance
Protein names Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 (EC 2.4.1.135) (Beta-1,3-glucuronyltransferase 1) (Glucuronosyltransferase P) (GlcAT-P) (UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase) (GlcUAT-P)
Gene names B3GAT1,B3GAT1 GLCATP
Protein family Glycosyltransferase 43 family
Mass 38256Da
Function FUNCTION: Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group. {ECO:0000250\|UniProtKB:O35789}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=3-O-(beta-D-galactosyl-(1->3)-beta-D-galactosyl-(1->4)-beta-D-xylosyl)-L-seryl-[protein] + UDP-alpha-D-glucuronate = 3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-seryl-[protein] + UDP + H(+); Xref=Rhea:RHEA:24168, Rhea:RHEA-COMP:12571, Rhea:RHEA-COMP:12573, ChEBI:CHEBI:15378, ChEBI:CHEBI:58052, ChEBI:CHEBI:58223, ChEBI:CHEBI:132090, ChEBI:CHEBI:132093; EC=2.4.1.135; Evidence={ECO:0000250\|UniProtKB:O35789};
Pathway PATHWAY: Protein modification; protein glycosylation.
Subellular location SUBCELLULAR LOCATION: [Isoform 1]: Golgi apparatus membrane {ECO:0000250\|UniProtKB:O35789}; Single-pass type II membrane protein {ECO:0000250\|UniProtKB:O35789}. Secreted {ECO:0000250\|UniProtKB:O35789}.; SUBCELLULAR LOCATION: [Isoform 2]: Golgi apparatus membrane {ECO:0000250\|UniProtKB:O35789}; Single-pass type II membrane protein {ECO:0000250\|UniProtKB:O35789}. Endoplasmic reticulum membrane {ECO:0000250\|UniProtKB:O35789}. Secreted {ECO:0000250\|UniProtKB:O35789}.
Tissues TISSUE SPECIFICITY: Mainly expressed in the brain.
Structure SUBUNIT: Homodimer. Interacts with SAR1A. {ECO:0000250\|UniProtKB:O35789}.
Post-translational modification PTM: The soluble form derives from the membrane form by proteolytic processing. {ECO:0000250\|UniProtKB:O35789}.
Target Relevance information above includes information from UniProt accession: Q9P2W7
The UniProt Consortium

Data

Human tonsil stained with anti-CD57 antibody using peroxidase-conjugate and DAB chromogen. Note intense cell membrane staining of natural killer cells.

Publications

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We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.