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Human Nivolumab ELISA Kit 60032

$570.00

Summary

  • ELISA kit for research use (RUO)
  • Reactivity: Nivolumab
  • Suitable for: ELISA
  • Time:3 hours
  • Sample types:Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
SKU: 60032 Category: Tags: , ,
Weight 1 lbs
Dimensions 9 × 5 × 2 in
target

Nivolumab

species reactivity

Human

applications

Indirect ELISA

assay type

Indirect & quantitative

available size

96 tests

sensitivity

0.938ng/ml

range

1.563-100ng/ml

Human Nivolumab ELISA Kit

kit
Assay type
ELISA
Research area
biologic
Sample type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Components
Break apart microtiter test strips each coated single wells 8 x 12 (96 Total)
Lyophilized Standard2 x vial
HRP Antibody(Concentrated, 100X)120 uL
Washing solution concentrate (25X)30 mL
Sample Dilution buffer20 mL
Antibody Dilution buffer10 mL
Stopping solution10 mL
TMB Substrate (ready-to-use)10 mL
Plate seals3
Storage
Store at 2-8°C.
target relevance
Nivolumab
Nivolumab biologic drug binds Homo sapiens PDCD1 Programmed cell death protein 1

Data

Standard Curve

X axis: log | Y axis: linear
Standard STD [ng/mL] OD1 OD2 Average OD Corrected OD
Blank #8 0 0.04 0.05 0.04 0
Standard #7 1.56 0.25 0.26 0.25 0.21
Standard #6 3.13 0.4 0.42 0.41 0.36
Standard #5 6.25 0.71 0.74 0.73 0.68
Standard #4 12.5 1.11 1.17 1.14 1.1
Standard #3 25 1.54 1.62 1.58 1.53
Standard #2 50 1.95 2.05 2 1.96
Standard #1 100 2.31 2.43 2.37 2.32

Precision

Sample n Mean Standard Deviation CV (%)
Intra Sample 1 20 3.01 0.13 4.32
Intra Sample 2 20 11.13 0.6 5.36
Intra Sample 3 20 48.42 2.54 5.24
Inter Sample 1 20 3.14 0.17 5.26
Inter Sample 2 20 11.55 0.59 5.14
Inter Sample 3 20 47.11 2.12 4.51

Recovery

Matrix Recovery Range (%) Average (%)
Serum(n=5) 87-104 92
EDTA Plasma(n=5) 92-101 96
HeparinPlasma(n=5) 87-105 95

Stability

37°C 1 Month (%) 2–8°C 6 Months (%) 2–8°C 12 Months (%)
80 95-100 85-98

Linearity

Sample 1:2 Dilution (%) 1:4 Dilution (%) 1:8 Dilution (%)
Serum(n=5) 85-104% 84-99% 82-96%
EDTAPlasma(n=5) 91-101% 85-91% 90-98%
Heparin Plasma(n=5) 85-105% 88-100% 82-100%

FAQ & Publications

Publications
pmid title authors citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

Relevant to this product
View Protocol  |  View PDF

Documents

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