| Weight | 1 lbs |
|---|---|
| Dimensions | 9 × 5 × 2 in |
| target | Quinolones |
| species reactivity | Universal |
| applications | Coated with Antigen, Competitive ELISA |
| assay type | Indirect & quantitative |
| available size | 96 tests |
| sensitivity | 0.469ng/ml |
| range | 0.781-50ng/ml |
Quinolones ELISA Kit 60066
$570.00
Summary
- ELISA kit for research use (RUO)
- Reactivity: Quinolones
- Suitable for: ELISA
- Time:3 hours
- Sample types:Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Quinolones ELISA Kit
| kit | ||||||||||||||||||||||
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| Assay type competition (cELISA) | ||||||||||||||||||||||
| Research area biologic | ||||||||||||||||||||||
| Sample type Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples | ||||||||||||||||||||||
Components
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| Storage Store at 2-8°C. |
| target relevance |
|---|
| Quinolones Quinolones biologic drug Quinolones antiboiotic |
| Protein names Quinolone antibiotic |
| Alternative names Quinolones, Fluoroquinolones, Quinolone antimicrobial agents |
| Function Quinolone antibiotics inhibit bacterial DNA gyrase and topoisomerase IV, resulting in impaired DNA replication, transcription, and repair, ultimately leading to bacterial cell death. |
| Description Quinolones are a class of synthetic broad-spectrum antibacterial agents that inhibit bacterial DNA replication through targeting DNA gyrase and topoisomerase IV. Fluoroquinolones are a major subclass characterized by the presence of a fluorine atom that enhances antibacterial activity. |
| Chemical description Antibacterial agent 4-quinolone nucleus 0: First-generation quinolones; 1: Fluoroquinolones |
| Biological role Antibacterial agent DNA gyrase inhibitor Topoisomerase IV inhibitor |
| Research relevance Quinolone antibiotics are common targets in food safety monitoring, residue analysis, environmental testing, and antimicrobial resistance studies. Antibodies against quinolones often exhibit cross-reactivity with multiple members of the quinolone and fluoroquinolone classes. |
| Keywords Antibiotic, Quinolone, Fluoroquinolone, DNA replication inhibition |
| Structure Quinolones are synthetic antibacterial compounds based on a 4-quinolone core structure. Fluoroquinolones contain a fluorine substituent that enhances antimicrobial activity. |
| Function Quinolones inhibit bacterial DNA gyrase and topoisomerase IV, preventing DNA replication and cell division. |
| Members Important members include nalidixic acid, ciprofloxacin, enrofloxacin, norfloxacin, ofloxacin, levofloxacin, moxifloxacin, and danofloxacin. |
| Applications Quinolones are widely monitored in food products, environmental samples, and pharmaceutical preparations due to their extensive use in human and veterinary medicine. |
| Research relevance Antibodies against quinolones are commonly used in ELISA, lateral flow, and other residue detection assays for food safety and environmental monitoring. |
Data
Standard Curve
X axis: log |
Y axis: linear
| Standard | STD [ng/mL] | OD1 | OD2 | Average OD | Corrected OD |
|---|---|---|---|---|---|
| Blank #8 | 0 | 2.09 | 2.15 | 2.12 | 0 |
| Standard #7 | 0.78 | 1.41 | 1.46 | 1.44 | -0.69 |
| Standard #6 | 1.56 | 0.83 | 0.86 | 0.84 | -1.28 |
| Standard #5 | 3.13 | 0.54 | 0.56 | 0.55 | -1.57 |
| Standard #4 | 6.25 | 0.4 | 0.41 | 0.4 | -1.72 |
| Standard #3 | 12.5 | 0.24 | 0.25 | 0.24 | -1.88 |
| Standard #2 | 25 | 0.13 | 0.13 | 0.13 | -1.99 |
| Standard #1 | 50 | 0.09 | 0.09 | 0.09 | -2.03 |
Precision
| Sample | n | Mean | Standard Deviation | CV (%) |
|---|---|---|---|---|
| Intra Sample 1 | 20 | 1.61 | 0.08 | 5.22 |
| Intra Sample 2 | 20 | 5.77 | 0.28 | 4.89 |
| Intra Sample 3 | 20 | 23.64 | 1 | 4.23 |
| Inter Sample 1 | 20 | 1.46 | 0.08 | 5.35 |
| Inter Sample 2 | 20 | 6.16 | 0.26 | 4.2 |
| Inter Sample 3 | 20 | 25.17 | 1.32 | 5.23 |
Recovery
| Matrix | Recovery Range (%) | Average (%) |
|---|---|---|
| Serum(n=10) | 87-104 | 97 |
| EDTAPlasma(n=10) | 85-102 | 97 |
| Heparin Plasma(n=10) | 93-103 | 99 |
Stability
| 37°C 1 Month (%) | 2–8°C 6 Months (%) | 2–8°C 12 Months (%) |
|---|---|---|
| 80 | 95-100 | 85-98 |
Linearity
| Sample | 1:2 Dilution (%) | 1:4 Dilution (%) | 1:8 Dilution (%) |
|---|---|---|---|
| Serum(n=10) | 96-104% | 86-97% | 85-102% |
| EDTAPlasma(n=10) | 85-101% | 83-98% | 84-100% |
| HeparinPlasma(n=10) | 82-100% | 82-98% | 82-94% |
FAQ & Publications
Publications
| pmid | title | authors | citation |
|---|---|---|---|
| We haven't added any publications to our database yet. | |||
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.
Protocols
| Relevant to this product |
|---|
| View Protocol | View PDF |
Documents
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| Please enter your product and batch number here. | ||
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