ICC/IF image goat anti GFP polyclonal antibody 1379

Western_blot image goat anti GFP polyclonal antibody 1379

goat anti-GFP polyclonal antibody 1379

$100.00 – $2,600.00

Antibody summary

Goat polyclonal to GFP
Suitable for: WB, ICC/IF, IHC
Reacts with: tagged fusion proteins
Isotype: IgG
100 µg, 25 µg, 1 mg

SKU: 1379parent Categories: antibody, epitope tag Tag: GFP

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	goat
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

goat anti-GFP polyclonal antibody 1379

antibody
Database link: P42212
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:1000-5000 IF/IHC: 1:5000
Immunogen Recombinant AcGFP expressed in and purified from E. coli
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control Goat polyclonal - Isotype Control

target relevance
Protein expression of GFP and GFP tagged proteins can be checked and quantified using this antibody in Western blotting. When imaging in situ, GFP fluorescence can be amplified by this antibody when used in conjunction with a suitable fluorescent label or secondary antibody. Click for more on: epitope tags and GFP
Protein names Green fluorescent protein
Gene names GFP,GFP
Protein family GFP family
Mass 26886Da
Function Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
Tissues Photocytes.
Structure Monomer.
Post-translational modification Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnology Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Green fluorescent protein can be mutated to emit at different wavelengths such as blue for BFP (when Tyr-66 is replaced by His), cyan for CFP (when Tyr-66 is replaced by Trp), and yellow for YFP (when Thr-203 is replaced by Tyr). Further generation of mutants led to more stable proteins (at 37 degrees Celsius for example) with brighter fluorescence and longer fluorescence lifetimes. Fluorescent proteins and their mutated allelic forms have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions (PubMed:17685514, PubMed:17685554, PubMed:8578587, PubMed:8707053, PubMed:9145105, PubMed:9154981, PubMed:9759496, PubMed:9782051). Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy (PubMed:17685514).
Target Relevance information above includes information from UniProt accession: P42212
The UniProt Consortium

Immunofluorescent analysis of transfected HEK293 cells with a GFP-construct in green stained with goat pAb to GFP, 1379, dilution 1:5,000 in red. The blue is Hoechst staining of nuclear DNA. The 1379 antibody reveals GFP protein expressed only in transfected cells, as a result transfected cells are appeared express both red and green signals and so appear an orange-golden color. Untransfected cells show neither signal.

Western blot analysis of HEK293 cell lysates using goat pAb to GFP, 1379, dilution 1:1,000, in green: [1] protein standard, [2] non-transfected control cells, [3] cells transfected with a GFP construct and [4] cells transfected with an mCherry construct. Strong band at ~27kDa corresponds to GFP protein detected only in cells transfected with GFP construct. This antibody does not recognize the mCherry protein.

Chromogenic immunostaining of formalin fixed paraffin embedded GFP transduced mouse brain with goat pAb to GFP, 1379, dilution 1:5,000, detected with DAB (brown) using the Vector Elite 8718C-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. Antibody 1379 specifically detected GFP positive cells in the cerebellum as expected for this model.

Above, mice transgenic for green fluorescent protein (GFP) under the Thy1 promoter express GFP in cerebral pyramidal cells. The image is of a 400µM maximum intensity optical section taken from an entire Thy1-GFP mouse brain cleared in the Lifecanvas lab. Lifecanvas scientists then stained the brain with EnCor goat polyclonal antibody to GFP 1379 coupled to a red dye. As expected, the green and red signals superimpose in the perikarya of the pyramidal cells which appear yellowish. The antibody also stains the apical dendrites which have a lower level of GFP expression, effectively amplifying the GFP signal. Mouse select on image for larger view.

Publications

pmid	title	authors	citation
13911999	Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea.	O SHIMOMURA, F H JOHNSON, Y SAIGA	J Cell Comp Physiol 59:223-39
12693991	A colourless green fluorescent protein homologue from the non-fluorescent hydromedusa Aequorea coerulescens and its fluorescent mutants.	Nadya G Gurskaya, Arkady F Fradkov, Natalia I Pounkova, Dmitry B Staroverov, Maria E Bulina, Yurii G Yanushevich, Yulii A Labas, Sergey Lukyanov, Konstantin A Lukyanov	Biochem J 373:403-8
11988576	Partitioning of lipid-modified monomeric GFPs into membrane microdomains of live cells.	David A Zacharias, Jonathan D Violin, Alexandra C Newton, Roger Y Tsien	Science 296:913-6
9759496	The green fluorescent protein.	R Y Tsien	Annu Rev Biochem 67:509-44
8703075	Crystal structure of the Aequorea victoria green fluorescent protein.	M Ormö, A B Cubitt, K Kallio, L A Gross, R Y Tsien, S J Remington	Science 273:1392-5

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.