ICC/IF image goat anti GFAP polyclonal antibody 8205

goat anti-GFAP polyclonal antibody 8205

$100.00 – $2,600.00

Antibody summary

Goat polyclonal to GFAP
Suitable for: WB, ICC/IF, IHC
Reacts with: human, mouse, rat
Isotype: IgG
100 µg, 25 µg, 1 mg

available sizes

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SKU: 8205parent Categories: antibody, cell marker Tag: GFAP

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	goat
isotype	IgG
clonality	polyclonal
concentration	1 mg/mL
applications	ICC/IF, IHC, WB
reactivity	human, mouse, rat
available sizes	1 mg, 100 µg, 25 µg

goat anti-GFAP polyclonal antibody 8205

antibody
Database link: human P14136 mouse P03995 rat P47819
Tested applications WB,IHC,IHC,ICC/IF
Recommended dilutions WB: 1:5000 IF/ICC 1:5000
Immunogen Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer PBS, 50% glycerol, 0.04% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgG
Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control Goat polyclonal - Isotype Control

target relevance
Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders. Click for more on: cell markers and GFAP
Protein names Glial fibrillary acidic protein (GFAP)
Gene names GFAP,GFAP
Protein family Intermediate filament family
Mass 49880Da
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subellular location Cytoplasm Note=Associated with intermediate filaments.
Tissues Expressed in cells lacking fibronectin.
Structure Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus).
Post-translational modification Phosphorylated by PKN1.
Involvement in disease Alexander disease (ALXDRD) [MIM:203450]: A rare disorder of the central nervous system. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death within the first decade. Infants with Alexander disease develop a leukodystrophy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course. Histologically, Alexander disease is characterized by Rosenthal fibers, homogeneous eosinophilic inclusions in astrocytes. Note=The disease is caused by variants affecting the gene represented in this entry.
Target Relevance information above includes information from UniProt accession: P14136
The UniProt Consortium

Data

Immunofluorescent analysis of mouse hippocampus section stained with goat pAb to GFAP, 8205, dilution 1:5,000 in green, and costained with mouse mAb to FOX3/NeuN, 2083, dilution 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic glial cells, while the FOX3/NeuN antibody specifically labels nuclei and proximal perikarya of neurons.

Chromogenic immunostaining of a formalin fixed paraffin embedded mouse cerebral cortex section with goat pAb to GFAP, 8205, dilution 1:10,000, detected with DAB (brown) using the Vector Elite 8718C-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In normal brain tissue, the GFAP antibody specifically labels astrocytes. It can also detect chronically activated microglia in neurodegenerative conditions. This antibody was raised against recombinant human GFAP expressed in and purified from E. coli. The human GFAP sequence was derived from NP_002046.1 which was inserted into the eukaryotic expression vector pET30a(+) which adds an N-terminal His tag and some other sequence which is underlined below.

Western blot analysis of brain lysates from different species using goat pAb to GFAP, 8205, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cortex, [3] rat cerebellum, [4] mouse cortex, [5] mouse cerebellum, [6] cow cortex, and [7] cow cerebellum. Strong band at about 50 kDa corresponds to GFAP protein. Smaller proteolytic fragments of GFAP are also detected on the blot.

Publications

pmid	title	authors	citation
37442620	Recovery from Traumatic Brain Injury Is Nociceptin/Orphanin FQ Peptide Receptor Genotype-, Sex-, and Injury Severity-Dependent.	Omar N Al Yacoub, Hibah O Awwad, Kelly M Standifer	J Pharmacol Exp Ther 389:136-149
17617407	CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failure.	Michael T Fitch, Jerry Silver	Exp Neurol 209:294-301
11138011	Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.	M Brenner, A B Johnson, O Boespflug-Tanguy, D Rodriguez, J E Goldman, A Messing	Nat Genet 27:117-20
7193676	Antibodies to neurofilament, glial filament, and fibroblast intermediate filament proteins bind to different cell types of the nervous system.	S H Yen, K L Fields	J Cell Biol 88:115-26
6799297	An immunofluorescence microscopical study of the neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brain.	G Shaw, M Osborn, K Weber	Eur J Cell Biol 26:68-82

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.