Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
host | goat |
isotype | IgG |
clonality | polyclonal |
concentration | 1 mg/mL |
applications | ICC/IF, IHC, WB |
available sizes | 1 mg, 100 µg, 25 µg |
goat anti-GFAP polyclonal antibody 8205
$100.00 – $2,600.00
Antibody summary
- Goat polyclonal to GFAP
- Suitable for: WB, ICC/IF, IHC
- Reacts with: human, mouse, rat
- Isotype: IgG
- 100 µg, 25 µg, 1 mg
goat anti-GFAP polyclonal antibody 8205
antibody |
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Database link: human P14136 mouse P03995 rat P47819 |
Tested applications WB,IHC,IHC,ICC/IF |
Recommended dilutions WB: 1:5000 IF/ICC 1:5000 |
Immunogen Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli. |
Size and concentration 25, 100, 1000µg and 1 mg/mL |
Form liquid |
Storage Instructions 2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles. |
Storage buffer PBS, 50% glycerol, 0.04% NaN3 |
Purity affinity purified |
Clonality polyclonal |
Isotype IgG |
Compatible secondaries donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689 donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704 donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709 donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705 |
Isotype control Goat polyclonal - Isotype Control |
target relevance |
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Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders. Click for more on: cell markers and GFAP |
Protein names Glial fibrillary acidic protein (GFAP) |
Gene names GFAP,GFAP |
Protein family Intermediate filament family |
Mass 49880Da |
Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. |
Subellular location Cytoplasm Note=Associated with intermediate filaments. |
Tissues Expressed in cells lacking fibronectin. |
Structure Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus). |
Post-translational modification Phosphorylated by PKN1. |
Target Relevance information above includes information from UniProt accession : P14136 |
The UniProt Consortium |
Data
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.There are 6 publications in our database for this antibody or clone. Here are the latest 5, for more click below.
pmid | title | authors | citation |
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37442620 | Recovery from traumatic brain injury (TBI) is Nociceptin/Orphanin FQ peptide (NOP) receptor genotype-, sex-, and injury severity-dependent | Al Yacoub ON, Awwad HO, Standifer KM. | J Pharmacol Exp Ther. 2023 Jul 13:JPET-AR-2023-001664. doi: 10.1124/jpet.123.001664. Online ahead of print. |
17617407 | CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failure | Fitch MT, Silver J. | Exp Neurol. 2008 Feb;209(2):294-301. doi: 10.1016/j.expneurol.2007.05.014. Epub 2007 May 31. |
11138011 | Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease | Brenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A. | Nat Genet. 2001 Jan;27(1):117-20. doi: 10.1038/83679. |
7193676 | Antibodies to neurofilament, glial filament, and fibroblast intermediate filament proteins bind to different cell types of the nervous system | Yen SH, Fields KL. | J Cell Biol. 1981 Jan;88(1):115-26. doi: 10.1083/jcb.88.1.115. |
6799297 | An immunofluorescence microscopical study of the neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brain | Shaw G, Osborn M, Weber K. | Eur J Cell Biol. 1981 Dec;26(1):68-82. |
Protocols
relevant to this product |
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Western blot IHC ICC |
Documents
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