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goat anti-GFAP polyclonal antibody 8205

$100.00$2,600.00

Antibody summary

  • Goat polyclonal to GFAP
  • Suitable for: WB, ICC/IF, IHC
  • Reacts with: human, mouse, rat
  • Isotype: IgG
  • 100 µg, 25 µg, 1 mg
SKU: 8205parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

goat

isotype

IgG

clonality

polyclonal

concentration

1 mg/mL

applications

ICC/IF, IHC, WB

available sizes

1 mg, 100 µg, 25 µg

Available product – goat anti-GFAP polyclonal antibody 8205

antibody
Database link:
human P14136
mouse P03995
rat P47819
Tested applications
WB,IHC,IHC,ICC/IF
Recommended dilutions
WB: 1:5000 IF/ICC 1:5000
Immunogen
Recombinant full length human GFAP isotype 1 expressed in and purified from E. coli.
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C for short term, for longer term at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS, 50% glycerol, 0.04% NaN3
Purity
affinity purified
Clonality
polyclonal
Isotype
IgG
Compatible secondaries
donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody 1689
donkey anti-goat IgG, H&L chain specific, biotin conjugated polyclonal antibody 1699
donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody 1704
donkey anti-goat IgG, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1709
donkey anti-goat IgG, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1705
Isotype control
Goat polyclonal - Isotype Control
target relevance
Glial Fibrillary Acidic Protein (GFAP) is a crucial cell marker widely employed in neuroscience and neurobiology research. It is an intermediate filament protein primarily found in astrocytes, a type of glial cell in the central nervous system. GFAP serves as a reliable marker for identifying and characterizing astrocytes in various experimental models and tissues. Immunohistochemistry and immunofluorescence techniques using antibodies targeting GFAP enable researchers to visualize and quantify astrocytic populations, gaining insights into their distribution, morphology, and function in the brain. Moreover, GFAP antibodies are instrumental in studying astrocyte reactivity, a phenomenon occurring in response to brain injury or neurodegenerative diseases. As astrocytes play critical roles in neural development, neurotransmitter regulation, and maintenance of the blood-brain barrier, GFAP has proven indispensable in advancing our understanding of glial biology and its contribution to neurological disorders.

Click for more on: cell markers and GFAP
Protein names
Glial fibrillary acidic protein (GFAP)
Gene names
GFAP,GFAP
Protein family
Intermediate filament family
Mass
49880Da
Function
GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
Subellular location
Cytoplasm Note=Associated with intermediate filaments.
Tissues
Expressed in cells lacking fibronectin.
Structure
Interacts with SYNM. ; [Isoform 2]: Interacts with PSEN1 (via N-terminus).
Post-translational modification
Phosphorylated by PKN1.
Target Relevance information above includes information from UniProt accession : P14136
The UniProt Consortium

Data

ICC/IF-image-goat-anti-GFAP-polyclonal-antibody-8205
Immunofluorescent analysis of mouse hippocampus section stained with goat pAb to GFAP, 8205, dilution 1:5,000 in green, and costained with mouse mAb to FOX3/NeuN, 2083, dilution 1:2,000, in red. The blue is Hoechst staining of nuclear DNA. Following transcardial perfusion of mouse with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45µM, and free-floating sections were stained with above antibodies. The GFAP antibody stains the network of astrocytic glial cells, while the FOX3/NeuN antibody specifically labels nuclei and proximal perikarya of neurons.
IHC-image-goat-anti-GFAP-polyclonal-antibody-8205
Chromogenic immunostaining of a formalin fixed paraffin embedded mouse cerebral cortex section with goat pAb to GFAP, 8205, dilution 1:10,000, detected with DAB (brown) using the Vector Elite 8718C-HRP detection and reagents with citra buffer retrieval. Hematoxylin (blue) was used as the counterstain. In normal brain tissue, the GFAP antibody specifically labels astrocytes. It can also detect chronically activated microglia in neurodegenerative conditions. Mouse select image for larger view.This antibody was raised against recombinant human GFAP expressed in and purified from E. coli. The human GFAP sequence was derived from NP_002046.1 which was inserted into the eukaryotic expression vector pET30a(+) which adds an N-terminal His tag and some other sequence which is underlined below.MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EFMERRRITS 60 AARRSYVSSG EMMVGGLAPG RRLGPGTRLS LARMPPPLPT RVDFSLAGAL NAGFKETRAS 120 ERAEMMELND RFASYIEKVR FLEQQNKALA AELNQLRAKE PTKLADVYQA ELRELRLRLD 180 QLTANSARLE VERDNLAQDL ATVRQKLQDE TNLRLEAENN LAAYRQEADE ATLARLDLER 240 KIESLEEEIR FLRKIHEEEV RELQEQLARQ QVHVELDVAK PDLTAALKEI RTQYEAMASS 300 NMHEAEEWYR SKFADLTDAA ARNAELLRQA KHEANDYRRQ LQSLTCDLES LRGTNESLER 360 QMREQEERHV REAASYQEAL ARLEEEGQSL KDEMARHLQE YQDLLNVKLA LDIEIATYRK 420 LLEGEENRIT IPVQTFSNLQ IRETSLDTKS VSEGHLKRNI VVKTVEMRDG EVIKESKQEH 480 KDVM 484Number of amino acids: 484Molecular weight: 55588.41Theoretical pI: 5.47Amino acid composition: Ala (A) 52 10.7% Arg (R) 49 10.1% Asn (N) 17 3.5% Asp (D) 28 5.8% Cys (C) 1 0.2% Gln (Q) 27 5.6% Glu (E) 63 13.0% Gly (G) 18 3.7% His (H) 15 3.1% Ile (I) 14 2.9% Leu (L) 58 12.0% Lys (K) 24 5.0% Met (M) 16 3.3% Phe (F) 9 1.9% Pro (P) 11 2.3% Ser (S) 28 5.8% Thr (T) 22 4.5% Trp (W) 1 0.2% Tyr (Y) 10 2.1% Val (V) 21 4.3%Total number of negatively charged residues (Asp + Glu): 91 Total number of positively charged residues (Arg + Lys): 73Ext. coefficient 20400 Abs 0.1% (=1 g/l) 0.367, assuming all pairs of Cys residues form cystinesExt. coefficient 20400 Abs 0.1% (=1 g/l) 0.367, assuming all Cys residues are reduced
WB-image-goat-anti-GFAP-polyclonal-antibody-8205
Western blot analysis of brain lysates from different species using goat pAb to GFAP, 8205, dilution 1:5,000 in green: [1] protein standard (red), [2] rat cortex, [3] rat cerebellum, [4] mouse cortex, [5] mouse cerebellum, [6] cow cortex, and [7] cow cerebellum. Strong band at about 50 kDa corresponds to GFAP protein. Smaller proteolytic fragments of GFAP are also detected on the blot.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 6 publications in our database for this antibody or clone. Here are the latest 5, for more click below.


pmidtitleauthorscitation
37442620Recovery from traumatic brain injury (TBI) is Nociceptin/Orphanin FQ peptide (NOP) receptor genotype-, sex-, and injury severity-dependentAl Yacoub ON, Awwad HO, Standifer KM.J Pharmacol Exp Ther. 2023 Jul 13:JPET-AR-2023-001664. doi: 10.1124/jpet.123.001664. Online ahead of print.
17617407CNS injury, glial scars, and inflammation: Inhibitory extracellular matrices and regeneration failureFitch MT, Silver J.Exp Neurol. 2008 Feb;209(2):294-301. doi: 10.1016/j.expneurol.2007.05.014. Epub 2007 May 31.
11138011Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander diseaseBrenner M, Johnson AB, Boespflug-Tanguy O, Rodriguez D, Goldman JE, Messing A.Nat Genet. 2001 Jan;27(1):117-20. doi: 10.1038/83679.
7193676Antibodies to neurofilament, glial filament, and fibroblast intermediate filament proteins bind to different cell types of the nervous systemYen SH, Fields KL.J Cell Biol. 1981 Jan;88(1):115-26. doi: 10.1083/jcb.88.1.115.
6799297An immunofluorescence microscopical study of the neurofilament triplet proteins, vimentin and glial fibrillary acidic protein within the adult rat brainShaw G, Osborn M, Weber K.Eur J Cell Biol. 1981 Dec;26(1):68-82.

Protocols

relevant to this product
Western blot
IHC
ICC

Documents

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