chicken anti-HA tag polyclonal antibody 9232

$100.00 – $2,600.00

Antibody summary

Chicken polyclonal to HA tag
Suitable for: WB, ICC/IF, ELISA
Reacts with: tagged fusion proteins
Isotype: IgY
100 µg, 25 µg, 1mg

available sizes

Clear

SKU: 9232parent Categories: antibody, epitope tag Tag: HA tag

Weight	1 lbs
Dimensions	9 × 5 × 2 in
host	chicken
isotype	IgY
clonality	polyclonal
concentration	1 mg/mL
applications	ELISA, ICC/IF, WB
reactivity	tagged fusion proteins
available sizes	1 mg, 100 µg, 25 µg

chicken anti-HA tag polyclonal antibody 9232

antibody
Database link: Sequence -YPYDVPDYA Derived from HA 118-127. Example strain H3N8 Q03909
Tested applications WB,ICC/IF,ELISA
Recommended dilutions user optimized
Application Notes This antibody reacts with YPYDVPDYA as determined by IEP and ELISA techniques. It is suitable for blotting, ELISA and IHC applications. Optimal working dilutions should be determined experimentally by the investigator.
Immunogen YPYDVPDYA (influenza hemagglutinin-HAepitope)
Size and concentration 25, 100, 1000µg and 1 mg/mL
Form liquid
Storage Instructions 2-8°C
Storage buffer PBS, pH 7.2, 0.09% NaN₃
Purity affinity purified
Clonality polyclonal
Isotype IgY
Compatible secondaries goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036 goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317 goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708 goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718 goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control Chicken polyclonal - Isotype Control

target relevance

HA tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify HA tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, HA tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and HA tag

Biotechnology
The HA tag, derived from the human influenza hemagglutinin protein, is a widely employed tool in recombinant protein expression and has become an invaluable asset in molecular biology research. When incorporated at the N- or C-terminus of a target protein, the HA tag serves as a short, immunogenic peptide sequence, enabling efficient detection, purification, and characterization of the expressed protein. Its small size minimizes disruption to the target protein???s structure and function, making it suitable for a diverse range of applications. The HA tag is specifically recognized by commercially available high-affinity antibodies, facilitating the precise and sensitive detection of the tagged protein in techniques like Western blotting and immunofluorescence. Additionally, the HA tag is compatible with affinity chromatography, enabling straightforward and effective purification of the tagged protein from complex cellular extracts. This versatility has made the HA tag a valuable tool for studying protein interactions, localization, and post-translational modifications, providing researchers with valuable insights into cellular processes and molecular mechanisms across various fields of biology and biotechnology.

Data

Immunofluorescent analysis of 4% paraformaldehyde (PFA)-fixed permeabilized, with 0.1% Triton X-100 in CHO cells transfected with GFP-HA constructs labeling with HA tag antibody at 5 µg/mL, followed by Goat Anti-chicken IgG (H&L), Alexa 594 conjugated antibody at 2.5 µg/mL (red). Nucleus was counterstained with DAPI (blue).

Publications

pmid	title	authors	citation
34321103	Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instability.	Sheldon Decombe, Fran�ois Loll, Laura Caccianini, K�vin Affannoukou�, Ignacio Izeddin, Julien Mozziconacci, Christophe Escud�, Judith Lopes	Epigenetics Chromatin 14:35
34228711	Synaptotagmin-7-mediated activation of spontaneous NMDAR currents is disrupted in bipolar disorder susceptibility variants.	Qiu-Wen Wang, Ying-Han Wang, Bing Wang, Yun Chen, Si-Yao Lu, Jun Yao	PLoS Biol 19:e3001323
33008920	N-Terminal Targeting of Regulator of G Protein Signaling Protein 2 for F-Box Only Protein 44-Mediated Proteasomal Degradation.	Harrison J McNabb, Stephanie Gonzalez, Christine S Muli, Benita Sj�gren	Mol Pharmacol 98:677-685
31991108	Treatment of a Mouse Model of ALS by In�Vivo Base Editing.	Colin K W Lim, Michael Gapinske, Alexandra K Brooks, Wendy S Woods, Jackson E Powell, M Alejandra Zeballos C, Jackson Winter, Pablo Perez-Pinera, Thomas Gaj	Mol Ther 28:1177-1189
31792216	A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansion.	Peter G Boone, Lauren K Rochelle, Joshua D Ginzel, Veronica Lubkov, Wendy L Roberts, P J Nicholls, Cheryl Bock, Mei Lang Flowers, Richard J von Furstenberg, Barry R Stripp, Pankaj Agarwal, Alexander D Borowsky, Robert D Cardiff, Larry S Barak, Marc G Caron, H Kim Lyerly, Joshua C Snyder	Nat Commun 10:5490

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.