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chicken anti-HA tag polyclonal antibody 9232

$100.00$2,600.00

Antibody summary

  • Chicken polyclonal to HA tag
  • Suitable for: WB, ICC/IF, ELISA
  • Reacts with: tagged fusion proteins
  • Isotype: IgY
  • 100 µg, 25 µg, 1mg
SKU: 9232parent Categories: , Tags: , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
host

chicken

isotype

IgY

clonality

polyclonal

concentration

1 mg/mL

applications

ELISA, ICC/IF, WB

available sizes

1 mg, 100 µg, 25 µg

chicken anti-HA tag polyclonal antibody 9232

antibody
Database link:
Sequence -YPYDVPDYA
Derived from HA 118-127. Example strain H3N8 Q03909
Tested applications
WB,ICC/IF,ELISA
Recommended dilutions
user optimized
Immunogen
YPYDVPDYA (influenza hemagglutinin-HAepitope)
Size and concentration
25, 100, 1000µg and 1 mg/mL
Form
liquid
Storage Instructions
2-8°C
Storage buffer
PBS, pH 7.2, 0.09% NaN3
Purity
affinity purified
Clonality
polyclonal
Isotype
IgY
Compatible secondaries
goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody 1688
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody 8036
goat anti-chicken IgY, H&L chain specific, FITC conjugated, Conjugated polyclonal antibody 4317
goat anti-chicken IgY, H&L chain specific, peroxidase conjugated polyclonal antibody, crossabsorbed 1708
goat anti-chicken IgY, H&L chain specific, biotin conjugated polyclonal antibody, crossabsorbed 1718
goat anti-chicken IgY, H&L chain specific, FITC conjugated polyclonal antibody, crossabsorbed 1723
Isotype control
Chicken polyclonal - Isotype Control
target relevance
HA tag is a small protein tag that minimally impacts protein structure. This antibody can be used to confirm expression and quantify HA tagged recombinant proteins in Western blotting and for their purification/copurification. When imaging in situ, HA tagged proteins can be identified by this antibody when used in conjunction with a suitable secondary antibody.

Click for more on: epitope tags and HA tag
Biotechnology
The HA tag, derived from the human influenza hemagglutinin protein, is a widely employed tool in recombinant protein expression and has become an invaluable asset in molecular biology research. When incorporated at the N- or C-terminus of a target protein, the HA tag serves as a short, immunogenic peptide sequence, enabling efficient detection, purification, and characterization of the expressed protein. Its small size minimizes disruption to the target protein???s structure and function, making it suitable for a diverse range of applications. The HA tag is specifically recognized by commercially available high-affinity antibodies, facilitating the precise and sensitive detection of the tagged protein in techniques like Western blotting and immunofluorescence. Additionally, the HA tag is compatible with affinity chromatography, enabling straightforward and effective purification of the tagged protein from complex cellular extracts. This versatility has made the HA tag a valuable tool for studying protein interactions, localization, and post-translational modifications, providing researchers with valuable insights into cellular processes and molecular mechanisms across various fields of biology and biotechnology.

Data

ICC/IF-image-chicken-anti-HA tag-polyclonal-antibody-9232
ICC/IF-image-chicken-anti-HA tag-polyclonal-antibody-9232

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

There are 17 publications in our database for this antibody or clone. Here are the latest 5, for more click below.


pmidtitleauthorscitation
34321103Epigenetic rewriting at centromeric DNA repeats leads to increased chromatin accessibility and chromosomal instabilityDecombe S, Loll F, Caccianini L, Affannoukoué K, Izeddin I, Mozziconacci J, Escudé C, Lopes J.Epigenetics Chromatin. 2021 Jul 28;14(1):35. doi: 10.1186/s13072-021-00410-x.
34228711Synaptotagmin-7-mediated activation of spontaneous NMDAR currents is disrupted in bipolar disorder susceptibility variantsWang QW, Wang YH, Wang B, Chen Y, Lu SY, Yao J.PLoS Biol. 2021 Jul 6;19(7):e3001323. doi: 10.1371/journal.pbio.3001323. eCollection 2021 Jul.
33008920N-Terminal Targeting of Regulator of G Protein Signaling Protein 2 for F-Box Only Protein 44-Mediated Proteasomal DegradationMcNabb HJ, Gonzalez S, Muli CS, Sjögren B.Mol Pharmacol. 2020 Dec;98(6):677-685. doi: 10.1124/molpharm.120.000061. Epub 2020 Oct 2.
31991108Treatment of a Mouse Model of ALS by In Vivo Base EditingLim CKW, Gapinske M, Brooks AK, Woods WS, Powell JE, Zeballos C MA, Winter J, Perez-Pinera P, Gaj T.Mol Ther. 2020 Apr 8;28(4):1177-1189. doi: 10.1016/j.ymthe.2020.01.005. Epub 2020 Jan 14.
31792216A cancer rainbow mouse for visualizing the functional genomics of oncogenic clonal expansionBoone PG, Rochelle LK, Ginzel JD, Lubkov V, Roberts WL, Nicholls PJ, Bock C, Flowers ML, von Furstenberg RJ, Stripp BR, Agarwal P, Borowsky AD, Cardiff RD, Barak LS, Caron MG, Lyerly HK, Snyder JC.Nat Commun. 2019 Dec 2;10(1):5490. doi: 10.1038/s41467-019-13330-y.

Protocols

relevant to this product
Western blot
ICC
ELISA

Documents

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