Skip to content

Humanized CD3ε monoclonal antibody ELISA Kit 60036

$570.00

Summary

  • ELISA kit for research use (RUO)
  • Reactivity: Humanized CD3ε mAb
  • Suitable for: ELISA
  • Time:3 hours
  • Sample types:Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
SKU: 60036 Category: Tags: , ,
Weight 1 lbs
Dimensions 9 × 5 × 2 in
target

Humanized CD3ε mAb

species reactivity

Human

applications

Indirect ELISA Sandwich

assay type

Indirect & quantitative

available size

96 tests

sensitivity

2.813ng/ml

range

4.688-300ng/ml

Humanized CD3ε monoclonal antibody ELISA Kit

kit
Assay type
ELISA
Research area
biologic
Sample type
Serum, Plasma, Cell Culture Supernatant, cell or tissue lysate, Other liquid samples
Components
Break apart microtiter test strips each coated single wells 8 x 12 (96 Total)
Lyophilized Standard2 x vial
HRP Antibody(Concentrated, 100X)120 uL
Washing solution concentrate (25X)30 mL
Sample Dilution buffer20 mL
Antibody Dilution buffer10 mL
Stopping solution10 mL
TMB Substrate (ready-to-use)10 mL
Plate seals3
Storage
Store at 2-8°C.
target relevance
anti-Humanized CD3ε antibody
an autoantibody against anti-Humanized CD3ε antibody generated in a administered subject

Data

Standard Curve

X axis: log | Y axis: linear
Standard STD [ng/mL] OD1 OD2 Average OD Corrected OD
Blank #8 0 0.08 0.09 0.09 0
Standard #7 4.69 0.11 0.12 0.12 0.03
Standard #6 9.38 0.18 0.18 0.18 0.1
Standard #5 18.75 0.23 0.24 0.24 0.15
Standard #4 37.5 0.42 0.43 0.43 0.34
Standard #3 75 0.8 0.82 0.81 0.73
Standard #2 150 1.41 1.45 1.43 1.34
Standard #1 300 2.13 2.19 2.16 2.08

Precision

Sample n Mean Standard Deviation CV (%)
Intra Sample 1 20 9.38 0.49 5.22
Intra Sample 2 20 35.21 1.51 4.3
Intra Sample 3 20 150.6 6.9 4.58
Inter Sample 1 20 9.21 0.42 4.59
Inter Sample 2 20 38.79 1.75 4.51
Inter Sample 3 20 149.5 6.2 4.15

Recovery

Matrix Recovery Range (%) Average (%)
Serum(n=5) 85-98 90
EDTA Plasma(n=5) 85-105 93
HeparinPlasma(n=5) 86-103 96

Stability

37°C 1 Month (%) 2–8°C 6 Months (%) 2–8°C 12 Months (%)
80 95-100 85-98

Linearity

Sample 1:2 Dilution (%) 1:4 Dilution (%) 1:8 Dilution (%)
Serum(n=5) 87-105% 89-101% 87-97%
EDTAPlasma(n=5) 88-104% 86-96% 87-100%
Heparin Plasma(n=5) 87-100% 86-101% 85-97%

FAQ & Publications

Publications
pmid title authors citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from the PubMed database provided by the United States National Library of Medicine at the National Institutes of Health.

Protocols

Relevant to this product
View Protocol  |  View PDF

Documents

#
Please enter your product and batch number here.

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.