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SARS-COV-2 Spike RBD Protein 4661

$300.00$1,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Arg319-Asn532
SKU: 4661parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

QHD43416

express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.

molecular weight

The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 36-40 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

SARS-COV-2 Spike RBD Protein 4661

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
No information
target relevance

Data

SPR with SARS-COV-2 Spike RBD Protein
Human ACE2, hFc Tag captured on Protein A chip, can bind SARS-COV-2 Spike RBD, His Tag with an affinity constant of 13.8nM as determined in a SPR assay (Biacore T200).
ELISA with SARS-COV-2 Spike RBD Protein
Immobilized SARS-COV-2 Spike RBD, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Human ACE2, hFc Tag with the EC50 of 7.5ng/ml determined by ELISA (QC Test).
HPLC of SARS-COV-2 Spike RBD Protein
The purity of SARS-COV-2 Spike RBD is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of SARS-COV-2 Spike RBD Protein
SARS-COV-2 Spike RBD on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

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